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A study was done on the feeding behaviour, development indices, and biochemical response of 4th instar larvae of Spodoptera littoralis in which seven cotton genotypes were used: Giza86, Giza88, Giza92, 10229 × Giza 86, H10, Suvin, and Karshenseki. Castor bean leaves were used as the control. All the tested cotton genotypes decreased the feeding behavior of S. littoralis larvae in terms of consumption percentages, consumption rate (CR), growth rates (GR), efficiency of conversion of ingested and digested food (ECI and ECD), approximate digestibility (AD), and feeding deterrence (FDI) compared to the control. Additionally, all the tested cotton varieties prolonged the time taken for larval and pupal duration and reduced both the pupation percentages and the weight of the resulting pupae, as compared to the control. Giza86 recorded the lowest values of both larval growth index and fitness (7.31 and 1.05, respectively) while the genotype Suvin recorded the lowest standardised growth index (0.020) more than other genotypes and the control that gave 10.16, 1.53, and 0.032, respectively. There was a significant inhibition in the level of both total soluble protein and total lipids, and activities of amylase and the trehalase enzymes in all the tested varieties, compared to that found in the control.
The carried out investigations evaluated ovicidal activity of mahlab, Prunus mahaleb L. kernel oil against cotton leafworm, Spodoptera littoralis (Boisd.). The chemical constituents of the fixed oil of mahlab were analyzed using gas-liquid chromatography (GLC). Timnodonic (33.07%), oleic (28.71%) and linoleic (24.35%) were the basic fatty acids, while the major hydrocarbon and sterol were found to be heneicosane (62.57%) and β-sitosterol (10.57%). The LC50 values for the one-day-old egg masses were found to be more susceptible than 3-day-old ones. Moreover, the leaf dip technique occurred to be more efficient than spraying technique. The results also showed abnormalities in the external morphology of egg shell, chorion surface, shell imprints and aeropyles of S. littoralis eggs treated with mahlab and KZ oils as compared to a control by using scanning electron microscope. Generally, the tested oils significantly reduced the activities of transaminase enzymes (AST and ALT), acid and alkaline phosphatases and total soluble protein except mahlab oil on acid phosphatase as compared to a control. Additionally, the oils of both mahlab and KZ oil affected some biological aspects such as incubation period, larval duration, larval mortality and pupal weight comparing to a control.
Effectiveness of 8 bio-rational insecticides, Dipel 2x, BioFly, Agrin, BioGaurd, Spinosad, Neemix, Mectin and Match were tested at recommended dose, half of recommended dose and quarter of recommended dose against 1st, 3rd and 5th instar larvae and the egg masses (24, 48 and 72 h old) of the Egyptian cotton leafworm Spodoptera littoralis (Boisd.) under laboratory conditions. All the bio-rational insecticides provided higher mortality in the first instar larvae comparing to the third and fifth instar larvae, although Match, Mectin and Spinosad showed also excellent efficacy against third larval stage of S. littoralis at all tested concentrations. Also, Match showed 100% mortality of fifth instar larvae at all tested concentrations. The mortality rates of S. littoralis eggs of different ages (24, 48 and 72 h old) when dipped in recommended dose of each insecticide diluted in water were investigated. At recommended dose of all insecticides eggs of different ages were highly affected and the reductions of hatchability were 83.4, 85.0 and 71.7%, respectively in Spinosad compared to the control. In general, eggs 48 and 72 h old were less sensitive than younger eggs 24 h old. The latent effect of bio-rational insecticides on egg hatchability of S. littoralis was observed only in Match and Neemix with the average being 55.0% and 51.6% respectively. Our results suggest that Match, Mectin and Spinosad are potentially effective compounds for control of S. littoralis.
The male reproductive tract of Lepidoptera is an ideal model for the study of the physiological role of peripheral clocks in insects. The latter are significant in the generation and coordination of rhythmic phenomena which facilitate the initial stages of sperm capacitation. This process requires the maintenance of pH in the upper vas deferens (UVD) aided by, among others, H+-ATPase. Our aim was to determine the potential involvement of carbonic anhydrase (CA) in this process, an enzyme tasked with generating protons subsequently utilized by H+-ATPase to acidify the UVD milieu in S. littoralis, during the time when the lumen of this organ is filled with sperm. We attempted to answer the question whether CA activity can be controlled by the biological oscillator present in the male reproductive tract of the cotton leafworm. Using PAGE zymography, the presence of CA was demonstrated in the UVD wall, but not in the luminal fluid nor in the sperm. Using histochemistry, it was shown that CA is active in the UVD epithelium, and that this activity varies throughout the day and is most likely controlled by an endogenous biological clock. Conversely, the application of CA inhibitors, acetazolamide and sodium thiocyanate, in conjunction with an analysis of H+-ATPase activity in the acidification the UVD environment shows that CA most likely does not play a direct role in the regulation of the pH in this organ.
This study assessed the insect pest infestation of tomato during 2011 and 2012 in three localities namely Bula Kuliye, Kajeri and Soye of Bama Local Government Area, Borno State, Nigeria. Fifty plants were randomly checked per farmers’ field for insect pest recovery and count. Sampling was carried out in five to nine farmers’ fields per locality. With 38-52 %, 9-62 %, 13-39 % and 5-9 % species composition respectively across localities, Helicoverpa armigera Hubner (Lepidoptera: Noctuidae) followed by Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) were more predominant insect pests of tomato in these localities over Tetranychus sp. (Acari: Tetranichidae) and Spodoptera litoralis (Boisd.) (Lepidoptera: Noctuidae). Total proportion of infested tomato plants per farmers’ field ranged from 19-36 %, and was significantly higher in Bula Kuliye and Kajeri than in Soye. Total number of insect pests per plant/leave and bored fruits per farmers’ field fell between 2-5 and 21-35, and likewise, was higher in Bula Kuliye and Kajeri than in Soye. Although tomato plant infestation and fruit damage were found to be moderate or high in these localities, future studies on the bionomics or population dynamics of these insects remain essential for establishing their economic status.
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