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In a pot experiment conducted in a phytotron, the effectiveness of foliar nutrition of spinach (Spinacia oleracea L.) with different magnesium salts with and without the addition of 0.5% CO(NH2)2 was studied. Magnesium was applied 3 times in the form of solutions of MgSO4 x 7H2O, Mg(NO3)2 x 6H2O, MgCl2 x 6H2O, C4H6O4Mg x 4H2O, compared to water as the control treatment. The obtained results showed that foliar feeding of spinach with inorganic magnesium salts was an efficient method for supplementing the Mg level in plants during the growing period. But the application of a metalo-organic complex in the form of magnesium acetate (C4H6O4Mg x 4H2O) at a concentration of 1.7%, in spite of a similar effect on leaf Mg content, induced phytotoxic symptoms in the form of chlorotic and necrotic spots on the leaves. The application of the solutions of inorganic magnesium salts had a significant effect, resulting in more intensive leaf gas exchange (stomatal conductance, transpiration and photosynthesis) and an increase in leaf yield. Magnesium sulphate affected the abovementioned processes in the most effective way, while magnesium acetate had a negative impact. Foliar feeding of spinach with the magnesium salts resulted in an increased leaf content of protein, chlorophyll, carotenoids, nitrates and proline, but a decrease in vitamin C content. The addition of urea to the applied magnesium salt solutions increased the plant gas exchange rates and the leaf content of protein, chlorophyll, carotenoids, nitrates and proline, but it decreased the content of vitamin C, potassium and magnesium.
The technological process of producing canned products brought about a decrease in the level of analysed discriminants by 1-63%, nitrites in New Zealand spinach being excepted. The greatest losses were recorded in the content of total acids and vitamin C and in the case of New Zealand spinach also of chlorophylls. During one-year storage of the two products an increase in the content of nitrites of 44-66% and a fall in the level of nitrates of 8-11% were noted. In spinach the content of chlorophylls was reduced 51% and in New Zealand spinach that of total acids and vitamin C 12-37%. After one-year storage the preserves of New Zealand spinach compared with those of spinach contained 35% less dry weight, 4-6% less nitrates and nitrites, 20-50% less starch, dietary fibre, total nitrogen, ash and total acids, 55% less chlorophylls, and 69% vitamin C. Both products showed good organoleptic quality.
Spinach and New Zealand spinach were compared as raw material for the, processing industry. The structure and quality of yields, certain phytometric traits of plants, and selected physicochemical indices were taken into consideration. The two species were harvested for analyses at two dates i.e., in spring and early autumn. Irrespective of the date of harvest, the spinach was characterized by a richer content of the investigated chemical compounds. The harvest date caused distinct differences in the level of the investigated components in the spinach and fairly small ones in the New Zealand spinach. The raw material of the two species harvested in spring contained more sugars, starch, total acids, and vitamin C and less total nitrogen, beta-carotene, and chlorophylls than that from the autumn harvest.
The proton pumping activity of phase-partitioning purified plasma membrane fraction from spinach leaves was tested in vitro in the presence of exogenous indole-3-acetic acid. The sensitivity of the H+ pumping activity to the auxin was changed after flowering induction. We investigated the effect of whole spinach leaf treatments with substances affecting the phosphatidylinositol diphosphate transduction pathway on the in vitro sensitivity modification by photoperiodic induction. A role of calcium ions was supported by studies on leaves treated with a specific Ca²⁺ chelator (EGTA), a synthetic Ca²⁺ ionophore (A23187) or with calcium channel blokers (verapamil, lanthan chloride). An experiment using the transduction pathway inhibitor, lithium chloride, indicated that the intracellular concentration of Ca²⁺ was increased by inositol triphosphate.
We investigated the effect of selenium form and dose on the total glutathione and non-protein -SH group contents in the edible spinach (Spinacia oleracea L.) and ground tomato (Lycopersicon esculentum Mill.) plants. Our experiments were carried out in a hydroponic culture. Selenium was added to the culture medium in its selenite (Na2SeO3 x 5H2O) and selenate (Na2SeO4) forms. Regardless of the selenium form, we observed an increase in the non-protein thiol content. The non-protein -SH group content depended on the form and dose of selenium as well as on the organ and plant species. Regardless of the selenium form, a higher content of non-protein -SH groups were found in the spinach biomass than in the tomato biomass. Selenite contributed to a larger accumulation of non-protein -SH groups in the roots, whereas selenate contributed to their accumulation in the shoots
The alkalinity of ash, content of selected mineral compounds, nitrates III and V, and soluble and total oxalates were determined in spinach and New Zealand spinach grown in the spring or autumn period in the aspect of using these crops as a raw material for the food processing industry. In 100 g fresh weight of spinach the alkalinity of ash was 17.4-18.9 cm3 1 M HCL. As for the analysed factors, the following values were determined: potassium 628-663 mg, phosphorus 34-59 mg, calcium 109-340 mg, magnesium 68-78 mg, sodium 24-30 mg, iron 4, mg, nitrates 11.0-165.0 mg, nitrites 0.06-0.08 mg, soluble oxalates 347-563 mg, and total oxalates 370-938 mg. The mean alkalinity of ash in fresh weight of New Zealand spinach was 12% lower than in spinach. Also the content of potassium was 41% lower, of phosphorus 49%, of calcium 52%, of magnesium 38%, of iron 9%, of soluble oxalates 32%, of total oxalates 40%, and of nitrites 53%. The content of sodium was 222% and of nitrates 92% higher.
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