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Microbial biotransformation of two phosphonoacetic acid derivatives bearing two stereomeric centres

100%

Szyszkowiak J., Majewska P.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

2013

tom 94

nr 4

Three strains of microorganisms: Bacillus subtilis, Serratia liquefaciens and Escherichia coli were tested as whole-cell biocatalysts for the kinetic resolution of isomers of two new phosphonoacetic acid derivatives. Used compounds possess two chiral centres – one at the carbon adjacent to both functional groups and the other at the phosphorus. Biocatalytic hydrolysis of 2-butyryloxy-2-(butoxyetoxyphosphinyl)acetic acid and 2-butyryloxy-2-(isobutoxyetoxyphosphinyl) acetic acid with whole cells of Bacillus subtilis produced corresponding hydroxyphosphonates with diastereoselectivity ranging from 50 to 60%.

Effect of concentrated microwave field on bacteria reduction and physical properties of egg white

67%

Wesierska E., Trziszka T.

Medycyna Weterynaryjna

2007

tom 63

nr 04

421-424

The aim of the study was to evaluate the optimal conditions to produce a significant reduction in bacteria population using a concentrated microwave field. The research with CMF was conducted in two different diluting agents: physiological solution and egg white. Using liquid egg white as one of the most sensitive materials it was important to find optimal conditions of pasteurization without destructive changes in egg white proteins activity. Bacteria strains were isolated from the surface of egg shells and identified using an ATB System (Merck). They were compared with ATCC strains. Microwave impulses were generated in groups separated by well matched periods. One of the main findings of the investigation was that the destruction of microbes was not associated with an increase in temperature; the temperature of material subjected to microwave was not higher than 44°C.

Ograniczanie wyników

1 BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

1 Medycyna Weterynaryjna

1 Majewska P.

1 Szyszkowiak J.

1 Trziszka T.

1 Wesierska E.

1 2013

1 2007

Wyniki wyszukiwania

Microbial biotransformation of two phosphonoacetic acid derivatives bearing two stereomeric centres

Effect of concentrated microwave field on bacteria reduction and physical properties of egg white