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  1. 17 Acta Parasitologica

  2. 2 Acta Biochimica Polonica

  3. 1 Annals of Agricultural and Environmental Medicine

  4. 1 Polish Journal of Food and Nutrition Sciences

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  1. 3 Fried B.

  2. 3 Swiderski Z.

  3. 2 Abdallah M. S.

  4. 2 Ibrahim H. M.

  5. 2 Karim A. M.

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  1. 2 2021

  2. 1 2020

  3. 2 2019

  4. 3 2017

  5. 1 2015

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1

Origin, differentiation and ultrastructure of egg envelopes surrounding the miracidia of Schistosoma mansoni

100%
Swiderski Z.
Acta Parasitologica
|
1994
|
tom 39
|
nr 2
2

Analysis of Shistosoma mansoni genes using the expressed sequence Tag approach

100%
Shabaan A. M., Mohamed M. M., Abdallah M. S., Ibrahim H. M., Karim A. M.
Acta Biochimica Polonica
|
2003
|
tom 50
|
nr 1
Expressed sequence tags (ESTs) are partial cDNA sequences read from both ends of random expressed gene fragments used for discovering new genes. DNA libraries from four different developmental stages of Schistosoma mansoni used in this study generated 141 ESTs representing about 2.5% of S. mansoni sequences in dbEST. Se­quencing was done by the dideoxy chain termination method. The sequences were submitted to GenBank for homology searching in nonredundant databases using Ba­sic Local Alignment Search Tool for DNA (BLASTN) alignment and for protein (BLASTX) alignment at the National Center for Biotechnology Information (NCBI). Among submitted ESTs, 29 were derived from λgt11 sporocyst library, 70 from λZap adult worm library, 31 from λZap cercarial library, and 11 from λZap female B worm library. Homology search revealed that eight (5.6%) ESTs shared homology to previ­ously identified S. mansoni genes in dbEST, 15 (10.6%) are homologous to known genes in other organisms, 116 (81.7%) showed no significant sequence homology in the databases, and the remaining sequences (2.1%) showed low homologies to rRNA or mitochondrial DNA sequences. Thus, among the 141 ESTs studied, 116 sequences are derived from noval, uncharactarized S. mansoni genes. Those 116 ESTs are im­portant for identification of coding regions in the sequences, helping in mapping of schistosome genome, and identifying genes of immunological and pharmacological significance.
3

Homology and analogy in egg envelopes surrounding miracidia of Schistosoma mansoni and coracidia of Bothriocephalus clavibothrium

100%
Swiderski Z.
Acta Parasitologica
|
1994
|
tom 39
|
nr 3
4

Immunological parameters of the Pomacea lineata Spix, 1827 (Mollusca: Caenogastropoda) exposed to Schistosoma mansoni Sambon, 1907

75%
Silva B. B. T. N., Alves L. C., Brayner F. A., Portela Jr N. C., Mendes Tenorio F. C. A., Silva Lira A. C., de Albuquerque Y. M. L., Neto C. J. C. L., Wanderley-Teixeira V., Teixeira A. A. C.
Acta Parasitologica
|
2019
|
tom 64
|
nr 1
5

Reduced [14C]-methionine uptake and fecundity in Schistosoma mansoni females treated with recombinant tumor necrosis factor alpha in vitro

75%
Haseeb M. A., Agrawal R., Fried B.
Acta Parasitologica
|
2017
|
tom 62
|
nr 1
6

Evaluation of praziquantel effectivenss after decades of prolonged use in an endemic area in Egypt

75%
Ghazy R. M., Tahoun M. M., Abdo S. M., El-Badry A. A., Hamdy N. A.
Acta Parasitologica
|
2021
|
tom 66
|
nr 1
7

Agricultural medical survey - a joint project in Egypt

75%
Emara M. F.
Annals of Agricultural and Environmental Medicine
|
1994
|
tom 01
|
nr 2
8

Identifying phenotypes involved in susceptibility to Schistosoma mansoni infection in F1B6CBA mice

63%
Villar L. P., Vicente B., Blanco-Gomez A., Castellanos A., Perez-Losada J., Muro A.
Acta Parasitologica
|
2014
|
tom 59
|
nr 3
Schistosomiasis is a disease with a strong genetic component influenced by socioeconomic and ecological factors. Epidemiological studies have identified several genetic regions involved in the schistosomiasis susceptibility. However, it is not well known what physiological traits are predisposing to the disease. The study of experimental infections in inbred mouse strains with variable genetic susceptibility to the disease offers a good opportunity to tackle this question. F1B6CBA hybrid between the most divergent strains was infected in order to characterize the immunophenotypes that correlate with the susceptibility of schistosomiasis disease in mice. Complete blood counts and immunophenotype were determined at 0, 3, 6, and 9 weeks post infection. Nine weeks after cercariae exposure, animals were perfused and worm recovery was assessed. A large number of hepatic lesions, a reduction in the eosinophil and basophil count in the acute phase of infection and the decreased number of monocytes, neutrophils and B-lymphocytes are phenotypes associated with increased susceptibility to S. mansoni infection.
9

Malondialdehyde; Lipid peroxidation plasma biomarker correlated with hepatic fibrosis in human Schistosoma mansoni infection

63%
Aziz I. A., Yacoub M., Rashid L., Solieman A.
Acta Parasitologica
|
2015
|
tom 60
|
nr 4
10

Metabolic profiling of Echinostoma caproni and Schistosoma mansoni in their definitive and intermediate hosts

63%
O’Sullivan C., Fried B., Sherma J.
Acta Parasitologica
|
2013
|
tom 58
|
nr 1
This review examines metabolic profiling of Schistosoma mansoni and Echinostoma caproni in their definitive and intermediate hosts. The earlier coverage of the literature on metabolic profiling was reviewed by Wang et al. 2010, Advances in Parasitology, 73, 373–404 and covered mainly studies using proton nuclear magnetic resonance spectroscopy. The methods focused upon in our review are mainly chromatographic. In the studies reviewed, various metabolites were analyzed in hosts infected with either E. caproni or S. mansoni and compared to the uninfected controls.
11

Exploring possibilities for an alternative approach in experimental schistosomiasis mansoni: the peritoneal cavity of mice

63%
Mati V. L. T., Bicalho R. S., Melo A. L.
Acta Parasitologica
|
2017
|
tom 62
|
nr 1
12

The protective role of toll-like receptor agonist monophosphoryl lipid A against vaccinated murine schistosomiasis

63%
Aly I., Ibrahim E. H., Hamad R. S., Sayed H. E. L., Attiyah S. M. N., E-Komy W., Ghramh H. A., Alshehri A., Alsyaad K. M., Alshehri M., Kilany M., Morsy K., El-kott A. F., Taha R.
Acta Parasitologica
|
2020
|
tom 65
|
nr 3
13

Spatial and seasonal distribution of Holochilus sciureus with Schistosoma mansoni infection in an endemic area for schistosomiasis in Brazil

63%
Carmo-Silva C. F., Teles-Reis A., Silva-Soares R. F., Mendes Rodrigues J. G., Sampaio Lira M. G., Nogueira R. A., Vegas-Melo D., Cardoso D. T., Miranda G. S., Silva-Souza N.
Acta Parasitologica
|
2019
|
tom 64
|
nr 4
14

Anti-angiogenic and anti-lymphangiogenic role of praziquantel and artemether in experimental mansoniasis

63%
El-Aal N. F. A., Hamza R. S., Magdy M.
Acta Parasitologica
|
2017
|
tom 62
|
nr 4
15

Effects of Schistosoma mansoni infection on lutein and beta-carotene concentrations in Biomphalaria glabrata snails as determined by quantitative high performance reversed phase thin-layer chromatography

63%
Dieterich N., Fried B., Sherma J.
Acta Parasitologica
|
2013
|
tom 58
|
nr 3
High performance thin-layer chromatography was used to determine the concentration of β-carotene and lutein in the whole body and digestive gland-gonad complex (DGG) of uninfected Biomphalaria glabrata snails and those infected with Schistosoma mansoni for 6 and 8 weeks. Pigments were extracted from the snails using acetone and separated on EMD Millipore reversed phase C-18 plates with concentration zone using petroleum ether-acetonitrile-methanol (1:1:2) mobile phase. After development, two yellow pigment zones, lutein and β-carotene, were identified with respective R f values of 0.55 and 0.13 and then quantified by densitometry. Statistical analysis of the weight percentages of each pigment showed a significant decrease (P < 0.05) in the concentration of β-carotene in the DGGs of infected B. glabrata at 6 and 8 weeks post-infection compared to the uninfected snails. No significant differences were seen in the concentrations of β-carotene in the whole body of the uninfected versus infected snail samples. Changes in the lutein concentration of the infected DGG and whole snail bodies were insignificant compared to the uninfected controls. In conclusion, larval S. mansoni infection caused a significant decrease in the β-carotene concentration of the DGG at 6 and 8 weeks post infection.
16

Cloning and characterization of a Schistosoma mansoni 1H and 30S clones as two tegumental vaccine candidate antigens

63%
Shabaan A. M., Mohamed M. M., Abdallah M. S., Ibrahim H. M., Karim A. M.
Acta Biochimica Polonica
|
2003
|
tom 50
|
nr 1
Two Schistosoma mansoni cDNA clones 30S and 1H were identified by immuno- screening of sporocyst 2gt11 library and by random sequencing of clones from 2Zap libraries, respectively. Clone 30S was one of 30 clones identified by an antibody raised against tegument of 3-h schistosomules. The clone was found to encode an 81 amino-acid protein fragment. It was expressed in Escherichia coli as a fusion protein of calculated molecular mass of about 35 kDa with C-terminus of Schistosoma japonicum glutathione-S-transferase (Sj26; about 26 kDa). The recombinant fusion protein was specifically recognized by serum of rabbits immunized with irradiated cercariae. Clone 1H is one of 76 expressed sequence tags derived from an adult worm library. It encodes the complete sequence of a tegumental membrane protein, Sm13. The 104 amino-acid open reading frame encodes a protein with a calculated molecular mass of about 11.9 kDa. Clone 1H was expressed in E. coli as an insoluble fusion pro­tein with Sj26 of about 40 kDa. In Western blots, the fusion protein was recognized by serum from rabbits vaccinated with irradiated cercariae but not by preimmune rabbit sera. The cloning, characterization and expression of those proteins are therefore po­tentially usefull for vaccine development.
17

Cell therapy as a new approach on hepatic fibrosis of murine model of Schistosoma mansoni-infection

51%
Alsulami M., Abdel-Gaber R.
Acta Parasitologica
|
2021
|
tom 66
|
nr 1
18

Levels of oxidative stress markers: correlation with hepatic function and worm burden patients with schistosomiasis

51%
Cunha G. M. M., Silva V. M. A., Bessa K. D. G., Bitencourt M. A. O., Macedo U. B. O., Freire-Neto F. P., Martins R. R., Assis C. F., Lemos T. M. A. M., Almeida M. G., Freire A. C. G.
Acta Parasitologica
|
2012
|
tom 57
|
nr 2
Schistosomiasis is caused by Schistosoma mansoni and is a public health problem in Brazil. The typical granulomatous lesion is associated with the increase in the oxidative damage by generation of free radicals. The aim of this work was to correlate some oxidative stress markers with the worm burden on carriers of schistosomiasis (n = 30) in the acute phase in comparison to healthy subjects (n = 30). The pro-oxidant parameter used was the colorimetric quantification of reactive substances to thiobarbituric acid, while the antioxidant markers used were blood content of reduced glutathione and determination of the activity of catalase. The worm burden was assessed by Kato-Katz method. The results pointed out that initially there was no difference in the catalase activity. However, there was a positive correlation between the increase in parasitic load and intensity of lipid peroxidation, and decrease in the content of reduced glutathione. Additionally, only the aspartate aminotransferase levels presented to be high, while there was a decrease in bilirubin level. Therefore, a possible association between the establishment of the oxidative stress in tissue and the parasitic load of Schistosoma mansoni is suggested.
19

Assessment of Akodon cursor (Rodentia, Sigmodontinae) as permissive host to Schistosoma mansoni infection: morphology of adult worms

51%
Machado-Silva J. R., Heisler Neves R., Rodrigues-Silva R., Figueiredo de Oliveira R. M., Maldonado Junior A.
Acta Parasitologica
|
2011
|
tom 56
|
nr 2
The natural infection with parasitic helminths is common in wild rodent populations. Once such interactions are better understood in the laboratory, it will be more feasible to extend the findings to infected hosts in nature. The flukes recovered from laboratory-infected Akodon cursor at 63 days post-infection were stained with hydrochloric carmine and individually mounted on glass slide as whole-mounts. Light and laser scanning confocal microscopy studies of adult male and female Schistosoma mansoni are reported. The parasites were examined morphologically and biometrically, which was obtained in a digital system for image analysis. Parameters used were: tegument thickness, digestive, excretory and reproductive systems. The overall conclusion of this experiment is that the morphological features of adult worm were similar to laboratory mice. It has been confirmed that the grass mouse is a permissive host to S. mansoni infection.
20

Essential role if Citrus reticulata and mirazid in treatment of Schistosoma mansoni infected mice: biochemical and parasitological studies

51%
Aly H. F., Aly S. A.
Polish Journal of Food and Nutrition Sciences
|
2006
|
tom 15/56
|
nr 4
This study was designed to evaluate the changes in adenylate levels, adenylate energy charge (AEC) and glycolytic enzymes in control and infected mice and treatment with Citrus reticulata or the oleo-resin extract from Myrrh of Commiphora molmol tree (Mirazid), as a new antischistosomal drug. Alternations in the hepatic content of adenine nucleotides (ATP, ADP and AMP), Pi, phosphate potential, AEC, glucose, glycogen, AMP-deaminase, adenosine deaminase and total protein contents were investigated in a trial to point out the effect of infection with Schistosoma mansoni parasite as a hypoxia inducer in mice liver tissues. Moreover, the role of Citrus reticulata and Commiphora (Mirazid), natural plant extracts in improving the energy status in S. mansoni infected mice was studied, since Citrus reticulata was previously reported to possess anti-leukemia, antimicrobial and antibacterial activities, whereas Commiphora extract (Mirazid) – antifasciolicidal ones.
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