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The ultrastructure and cytochemistry of Mehlis' gland and egg-shell formation in Schistosoma haematobium

100%

Swiderski Z., Moczon T., Eklu-Natey D. T.

Wiadomości Parazytologiczne

1998

tom 44

nr 3

Schistosoma haematobium: cytochemistry of the Mehlis' gland and of the ootype wall

100%

Moczon T., Swiderski Z.

Acta Parasitologica

2002

tom 47

nr 4

Secretory granules produced by the Mehlis' gland and by the wall of the distal ootype in Schistosoma haematobium females, were examined at the ultrastructural level by means of several cytochemical methods. Strong staining, due to a relatively high percentage of carbohydrates in the granules, was observed in polymethacrylate sections treated with a phosphotungstic acidhydrochloric acid mixture at pH ≈ 0.5. Staining with ferric chloride-mixed-diamine mixtures (the LID and the HID reaction) showed no detectable level of oligosaccharide carboxyl and sulphate groups. A periodic acid-thiocarbohydrazide-silver proteinate reaction which was performed on epoxy sections, revealed a relatively high level of glycopyranosidic 1,2-diol groups in the granules. These results as well as the sensitivity of the secretory granules to the proteolytic action of papain indicated that the secretions produced by the Mehlis' gland and by the ootype wall were neutral glycoproteins.

Studies on vesical schistosomiasis among rural Ezza farmers in the Southwestern border of Ebonyi State, Nigeria

67%

Anosike J. C., Oguwuike U. T., Nwoke B. E. B., Asor J. E., Ikpeama C. A., Nwosu D. C., Ogbusu F. I.

Annals of Agricultural and Environmental Medicine

2006

tom 13

nr 1

Studies on vesical schistosomiasis and its snail vectors were carried out between October 2001–May 2002 among rural Ezza farmers inhabiting the southwestern border of Ebonyi State, Nigeria. The people are predominantly farmers. Of the 2,104 urine specimens examined in 10 communities, 466 (22.1%) comprising 305 (23.7%) men and 161 (19.7%) women were infected with visible haematuria as the predominant presenting symptom. Ezza people associate bloody urine with sexually transmitted diseases. There were no significant differences in the prevalence rates amongst various villages and sexes (p>0.05). There was a gradual increase in the disease prevalence as the subjects’ age increases. About 78.3% of the infected persons are aged 0–20 years. Statistical analysis revealed that the prevalence, intensity and visible haematuria were significantly more (p<0.05) in subjects under the age of 20 than subjects above 20. Among the infected population, 183 (39.3%) and 283 (60.7%) were excreting 50 eggs/10 ml urine and above 50 eggs/10 ml urine respectively. Lack of visible haematuria is a more valid indicator of the absence of vesical schistosomiasis. Of the various snails collected during malacological survey, mainly B. globosus were infected. Possible control measures are discussed.

Ograniczanie wyników

1 Acta Parasitologica

1 Annals of Agricultural and Environmental Medicine

1 Wiadomości Parazytologiczne

2 Moczon T.

2 Swiderski Z.

1 Anosike J. C.

1 Asor J. E.

1 Eklu-Natey D. T.

1 Ikpeama C. A.

1 Nwoke B. E. B.

1 Nwosu D. C.

1 Ogbusu F. I.

1 Oguwuike U. T.

1 2006

1 2002

1 1998

Wyniki wyszukiwania

The ultrastructure and cytochemistry of Mehlis' gland and egg-shell formation in Schistosoma haematobium

Schistosoma haematobium: cytochemistry of the Mehlis' gland and of the ootype wall

Studies on vesical schistosomiasis among rural Ezza farmers in the Southwestern border of Ebonyi State, Nigeria