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To test whether the internal transcribed spacer 2 (ITS2) region is an effective marker for using in authenticating of the Schisandra chinensis at the species and population levels, separately. And the results showed that the wild populations had higher percentage of individuals that had substitution of C→A at site 86-bp than the cultivated populations. At sites 10-bp, 37-bp, 42-bp and 235-bp, these bases of the Schisandra sphenanthera samples differed from that of S. chinensis. Two species showed higher levels of inter-specific divergence than intra-specific divergence within ITS2 sequences. However, 24 populations did not demonstrate much difference as inter-specific and intra-specific divergences were concerned. Both S. chinensis and S. sphenanthera showed monophyly at species level, yet the samples of different populations shown polyphyly at population level. ITS2 performed well when using BLAST1 method. ITS2 obtained 100% identification success rates at the species level for S. chinensis, with no ambiguous identification at the genus level for ITS2 alone. The ITS2 region could be used to identify S. chinensis and S. sphenanthera in the “Chinese Pharmacopoeia”. And it could also correctly distinguish 100% of species and 100% of genera from the 193 sequences of S. chinensis. Hence, the ITS2 is a powerful and efficient tool for species identification of S. chinensis.
Herba Polonica
|
2002
|
tom 48
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nr 3
146-155
From pharmacological and clinical studies is concluded, that a basis activity of Schisandra chinensis is adaptogenic action, especially influence on immunostimulatory activity, decrease of CNS activity, increase of endurance on exercise, increase of ability of learn and memory and increase of antioxidant activity. The another important and good documented activities of this plant are hepatic tissue protection, anticancer and cardiac activity, influence on blood vascular system, as well as antimicroorganisms and antiparasitic activity. It is necessary make a note, that fruits and senem, and extracts from them distinguish oneself low toxicity and then a long time may be used in therapy.
In its country of origin Schisandra chinensis is dubbed the five-flavour fruit, as its peel is sweet, its flesh is sour, its seeds are acrid and when ground, with time grow salty. The fruit originated from northern China and Korea, but can be grown in Poland too. Its healing properties rank it within the top fifteen plants used for this purpose. It stimulates and tones the nervous system in states of mental and physical tiredness, increased sleepiness, and low pressure; it increases the ability to see in the dark, regulates the functioning of the heart and stomach, increases the potential to work under difficult conditions.
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