The aim of this research is a genetic mapping of the STS markers derived from a model legume Medicago truncatula Gaertn. in lupin crop (Lupinus angustifolius L.). This study was undertaken within the framework of VI EU „Grain Legumes Integrated Project” (GLIP). The mapping population consists of 89 recombinant inbred lines of a cross between a domesticated line and a wild type (83A:476 x P27255). STS markers were generated by the PCR, using primer pairs designed on the basis of M. truncatula and P. sativum genomic sequences. Up till now 257 primer pairs designed and delivered by the Hungarian partner of the project have been tested. Most of the primers amplified a monomorphic product. In the case of 29 detected polimorphic products, segregation of alleles could be analyzed in the whole mapping population. 22 of these markers were located on the genetic linkage map based on the MFLP markers constructed by the Australian group. The position of the remaining 6 markers is still uncertain. Supplementing the published map with the STS markers increased its length to 1953 cM and the average marker distance into 4.1 cM.