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1

Comparative scanning studies on cockerell (Gallus domesticus) spermatozoa with the use of SEM and AFM method

100%

Adamski R., Kassner J.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2008

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tom 50

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nr 1

2

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Hair types in Polish selected taxa of Potentilla subsect. Collinae [Rosaceae]

86%

Kolodziejek J.

Acta Societatis Botanicorum Poloniae

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2008

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tom 77

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nr 3

217-224

Leaf hair types in Polish selected taxa from Potentilla subsect. Collinae Juz., i.e. P. collina Wibel s.str., P. silesiaca Uechtr. P. thyrsiflora Hillsen ex Zimmeter, P. wimanniana Gilnther and Schummel, P. leucopolitana P.J. Milller and P. leucopolitana P.J. Milller x P. incana P. Graebner were studied. A total of five unicellular hair types could be distinguished, based on the structure of the hair: straight, involuted, crispate, curved and stellate. The occurrence of involuted, curved and stellate hairs was observed for the first time for the majority of investigated taxa. There is a variation in density, position, as well as in the number of arms of stellate hairs. Although they are difficult to see without a stereo microscope, these differences seem to be systematically important. The study of hair types on surfaces of leaves supports the opinion that P. collina s.lato is a hybrid between P. argentea L. s.la-to, P. tabernaemontani Ascherson and P. incana P. Gaertner. Hairs form consistent characters which are highly suited for systematic purposes. The key to Polish taxa based on leaf hair types is given as well.

3

Comparative morphology and anatomy of the leaves of Ginkgo biloba L. cultivars

86%

Klimko M., Korszun S., Bykowska J.

Acta Scientiarum Polonorum. Hortorum Cultus

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2015

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tom 14

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nr 4

The article presents the results of research on the morphology and anatomy of the leaves of 21 cultivars (including 10 Polish cultivars) and two clones of Ginkgo biloba. Leaves from long shoots were collected at the Department of Dendrology and Nursery, Poznań University of Life Sciences, Poland. A light microscope and scanning electron microscope were used for observations. Eight morphological traits were analysed in the leaves, including the lamina and petiole. The research revealed that there were significant differences between the leaves of individual cultivars and that they differed in the length, width of the lamina and the length of petioles to a much greater extent than publications had described it so far. There were significant differences between the adaxial and abaxial epidermis of all the taxa, i.e. in the cuticle ornamentation, in the protrusive secondary sculpture (absence of papillae), the position and presence of stomata (occasionally on the adaxial leaf surface), the absence of the peristomatal ring and the thickness of the epidermis. Anatomical investigations revealed that the leaves of Ginkgo cultivars and clones under study were bifacial and the multi-layered mesophyll was diversified into spongy and palisade parenchyma. The research findings may be used for the identification of Ginkgo biloba cultivars, and the epicuticular traits may be useful for the identification and classification of fragments of fossil leaves. The article includes descriptions and illustrations of several new quantitative and qualitative characters of petiole and lamina which have not been published previously.

4

The SEM study on brush cells on surface of tongue in cat's embryos

86%

Jackowiak H.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2008

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tom 50

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nr 1

5

Procedures for obtaining optimal SEM images of coccolithophore debris in coccolith limestones

86%

Ciurej A.

Acta Palaeontologica Polonica

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2010

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tom 55

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nr 1

Coccolith debris in fossil zooplankton faecal pellets and the mode of its preservation are the unique source of data on the mode of feeding and digestion by ancient zooplankters. The animals are virtually absent in the fossil record in another form than their coprolites. However, minute structural details of coccospheres and their debris in the coccolith limestone are much less legible than in modern sediments. This paper presents how clear SEM images of details of coccolith plates in complete and dismembered coccospheres from fossil zooplankton faecal pellets can be obtained from thin sections of coccolith limestone. The images allow us to study the structural and compositional details of coccolith plates as well as their arrangements within the coccospheres and fossil faecal pellets.

6

Micromorphology of pollen grains of fruit trees of the genus Prunus

86%

Chwil M.

Acta Scientiarum Polonorum. Hortorum Cultus

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2015

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tom 14

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nr 4

The micromorphological characteristics of the pollen grain sculpture is one of the most important diagnostic traits in plants. Pollen grains in various species of the subfamily Prunoideae are characterized by variations in size, shape and exine sculpture. In the present work, for the first time, the micromorphology of pollen grains of fourteen cultivars from five fruit tree species of the genus Prunus was compared. Morphometric observations and analysis of pollen grains were performed using light and electron scanning microscopy. In terms of size, the pollen grains studied were classified as medium sized and large. Their shape was determined to be prolate, subprolate, or prolate spheroidal. The striae in the exine of P. armeniaca run parallel and can be branched or curved. The sculpture of P. persica grains shows elongated extending paralel regular and irregular striae, dichotomously branched. Regularly arranged, or at places branched, striae are found in the exine of P. avium pollen grains. The ornamentation of P. cerasus grains is composed of elongated striae, sometimes slightly curved or with a tendency to intertwine. The exine of P. domestica grains is characterized by forked, arched striae. The stria thickness differs significantly between cultivars within the species, while the groove width differs only between some species and cultivars. The obtained results on the exine sculpture of pollen grains can be used in the taxonomy of species of the genus Prunus.

7

A new approach to air pollution determination using annual rings: dendro-chemical elemental analysis of annual rings by SEM-EDS

86%

Nuhoglu Y.

Polish Journal of Environmental Studies

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2006

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tom 15

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nr 1

This paper presents a new approach to air pollution determined through the use of elemental analysis of the annual tree rings (ATRs) of Turkish red pine (Pinus brutia Ten.). For this aim, every annual ring width was measured on the cross-section wheels of Turkish red pine supplied from ten research points around the Kemerköy (Gökova) thermal power plant (KTPP). The fly ash collected from the surfaces of the needles and the annual growth ring of Turkish red pine in the polluted points were investigated by energy dispersive spectrometer (EDS). The elemental analysis of the annual rings were performed considering the fly ash elemental composition because the main pollutant source was the fly ash emitted from KTPP chimney smoke. With EDS analyses, some elements such as Al, Si, Mg, K, Fe, Ca, S, Zn, Ti, and Nb were determined in the fly ash. These elements accumulated in ATRs from the KTPP fly ash. They not only accumulated in annual rings formed after KTPP began operation but also accumulated in ATRs formed before KTPP started. It was confirmed that these elements accumulated in the annual rings affected by KTPP much more than the control ATRs. This paper proved that SEM-EDS, a new approach/analytical method to air pollution determination through ATRs, has been successfully applied to dendro-chemical elemental analysis of annual tree rings.

8

Micromorphology of stamens of some species of the genus Sansevieria petagna (Asparagaceae)

72%

Klimko M., Wysakowska I., Wilkin P., Wiland-Szymanska J.

Steciana

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2017

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tom 21

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nr 2

The paper presents in the first time the results of a micromorphological study on stamens of Sansevieria species. Flowers of 15 species obtained from herbarium specimens deposited at the Royal Botanic Gardens, Kew, the Botanical Garden Berlin-Dahlem and the Botanical Garden in Poznań were studied. Observations were conducted under a light and a scanning electron microscope. The study revealed significant differences between the outer and inner surfaces of anthers. All species have a well-defined endothecium of enlarged cells with a U-shaped or helical secondary wall thickening. The article includes descriptions and illustrations of several quantitative and qualitative features of anthers and filaments of some Sansevieria species. Our study indicates that stamen micromorphology may be taxonomically significant.

9

Sequences of epicuticular wax structures along stems in four selected tree species

72%

Tomaszewski D., Zielinski J.

Biodiversity: Research and Conservation

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2014

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tom 35

10

New records of Dactylobiotus parthenogeneticus Bertolani, 1982 provide insight into its genetic variability and geographic distribution

72%

Pogwizd J., Stec D.

Folia Biologica

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2020

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tom 68

|

nr 2

11

Morphology of the first instar larvae of two species of Taxigramma Perris, 1852 (Diptera: Sarcophagidae: Miltogramminae)

72%

Szpila K., Pape T.

Polish Journal of Entomology

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2013

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tom 82

|

nr 4

12

A comparative LM and SEM study of the structure of the mucosal glands of the gallbladder in two species of canids: the dog and the Chinese raccoon dog

72%

Jackowiak H., Godynicki S.

Folia Morphologica

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2006

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tom 65

|

nr 2

105-110

The studies were performed on the mucosa of the body of the gallbladder in the dog and Chinese raccoon dog, species belonging to the Canidae family. The mucosal glands in both species mostly have the form of alveolar or short tubular secretory units without excretory ducts and are situated in the middle part at the bottom of the crypts surrounded by folds of the mucosa. Sporadically we observed the mucous intraepithelial glands. The results of the light and scanning electron microscopic observations indicate interspecies differences in the density, type and size of secretory units and also their openings. In the raccoon dog the number of secretory units is 30 times greater than in the dog and the units are predominantly simple glands with small openings. In the dog mostly 2 or 3 secretory units with common wide openings were observed. The SEM images of the NaOH macerated mucosa of the gallbladders showed a connective tissue framework around the glands composed of flat lamina with an irregular pattern of fine collagen fibres and numerous fenestrations. The collagen network around the openings of the glands is more compact and provides mechanical support for the glands of the gallbladder.

13

Meriderma species (Myxomycetes) from the Polish Carpathians: a taxonomic revision using SEM-visualized spore ornamentation

72%

Janik P., Ronikier A.

Acta Societatis Botanicorum Poloniae

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2016

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tom 85

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nr 1

Meriderma represents a recently described genus of nivicolous myxomycetes with high morphological variability. Due to many complications in its taxonomy and species recognition in the past, the group was considered a morphologically variable complex. Recent clarifications and recognition of morphological boundaries into species and morphotypes has fostered a classification revision of specimens found in the Carpathians. Material used in this study was systematically collected in the Polish part of the Carpathians from 2004 to 2009. As a result of micro- and macroscopic observations of 54 collections, we recorded nine taxa of Meriderma. Seven of these (all but M. carestiae and M. cribrarioides) are the first records for Poland and for the Carpathians overall. Our observations based on analysis of spore ornamentation by SEM are in accordance with recently proposed classification and confirm segregation of taxa based on spore ornamentation pattern.

14

Pollen morphology of selected European species of the genus Allium L. (Alliaceae)

72%

Wronska-Pilarek D., Halbritter H., Krzyminska A., Bednorz L., Bocianowski J.

Acta Scientiarum Polonorum. Hortorum Cultus

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2016

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tom 15

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nr 4

The pollen morphology of eight species from three subgenera and five sections of the genus Allium L. was studied by LM and SEM (i.e.: A. angulosum, A. carinatum, A. senescens subsp. montanum, A. oleraceum, A. scorodoprasum, A. ursinum, A. victorialis and A. vineale). The material came from natural sites of these species located in Poland, Czech Republic, Austria and Italy. For measurements a sample consisted of 30 pollen grains. In total, 240 pollen grains were analysed. They were analysed in respect to six quantitative features (i.e.: length of long axis – LA, length of short axis – SA, thickness of exine along long axis – Ex, SA/LA and Ex/LA ratios and length of sulcus) and the following qualitative ones: pollen outline and shape, exine ornamentation. Taxonomic value of these pollen features is considerable, especially on the sections level. On the basis of these features, it is impossible to distinguish individual Allium species but only their groups. The examined features were characterized by moderate (LA, SA and SA/LA) or high variability (Ex, Ex/LA). Among studied species the lowest variability was found in A. victorialis and the highest in A. vineale and A. oleraceum.

15

SEM studies on sporangia and pollen morphology of Gingko biloba cultivars

72%

Klimko M., Bykowska J., Korszun S.

Steciana

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2016

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tom 20

|

nr 3

The present study was conducted to illustrate the micromorphology of mature sporangia and pollen grains of four Ginkgo biloba cultivars: ‘Horizontalis’, ‘Lasiocarpa’, ‘Pragensis’ and ‘Tubifolia’ and is a continuation of earlier investigations on this subject. The sporophylls bear typically two pendulous sporangia, whereas three were found in one cultivar. The sporangia varied in shape from oval, elliptical elongate to boat-shaped and dehisced along a longitudinal slit. The sporangial walls are multilayered. The sculpture of the sporangium is rugose covered with wax crystals (tubules) and the endothecium shows clearly visible fibrous thickening on the wall. The pollen grains are monosulcate, the non-apertural surface is irregularly ridged (striate) or rugulate, with perforations and the germinal aperture (furrow) is covered with verrucae. The current study did not differentiate significantly four cultivars based on sporangia and pollen morphology. The micromorphological traits may be useful for identification and classification of fragments of fossil sporangia and pollen grains.

16

Micromorphology of the epidermis and anatomical structure of the leaves of Scorzonera hispanica L.

72%

Chwil M., Krawiec M., Krawiec P., Chwil S.

Acta Societatis Botanicorum Poloniae

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2015

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tom 84

|

nr 3

In Poland Scorzonera hispanica L. is rare in the wild. This species is used as a vegetable and medicinal plant. Currently, attempts are being made to introduce this plant into cultivation in Poland. In this study, comparative analyses were conducted of the epidermis surface micromorphology and anatomical structure of the leaves of S. hispanica 'Maxima' and 'Meres'. The investigations were performed using fluorescence, light and scanning electron microscopy. The cuticle on the surface of epidermal cells is smooth or striated. In the epidermis, there are anomocytic stomata. The stomatal index in the epidermis of the studied cultivars is 9.3-11%. In the midrib of the leaf, there is an aerial cavity which occupies a substantial area. In this place, cracking and breaking of the leaf blade were observed. Over the aerial cavity under the adaxial epidermis, there is a single layer of collenchyma cells and 1-2 rows of parenchyma cells. Tangential collenchyma is also present between the abaxial epidermis and large vascular bundles located in the midrib and on both sides of the large vascular bundles in the lamina. This tissue strengthens the leaf margin. The mesophyll cells located in the abaxial epidermis of the midrib form protrusions surrounding the large vascular bundles. The leaves of S. hispanica represent the equifacial type.

17

Orobanche elatior and O. kochii (Orobanchaceae) in Poland: distribution, taxonomy, plant communities and seed micromorphology

58%

Piwowarczyk R., Krajewski L.

Acta Societatis Botanicorum Poloniae

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2015

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tom 84

|

nr 1

Species of the genus Orobanche (Orobanchaceae), parasitic on Centaurea in Central Europe, were previously considered to belong to the O. elatior group. At present, the taxon is differentiated into two species, O. elatior Sutton and O. kochii F.W. Schultz. The paper presents for the first time the distribution of O. elatior and O. kochii in Poland based on a critical revision of herbarium and the literature data, as well as the results of field studies conducted between 1999 and 2014. The majority of the species’ localities are in south Poland: Silesia-Cracow, Małopolska and the Lublin Uplands. The distribution of both species in Poland is mapped and chronologically organized, and is thus the most recent in Europe. The taxonomy, host preferences, and ecology are also discussed. Seeds of both species were also investigated using light and scanning electron microscopy, which resulted in the designation of diagnostic features. The new color form of O. kochii f. citrina is described and illustrated. An account of all revised herbarium specimens collected from Poland, deposited in Poland and neighboring countries, is presented.

18

Anatomical study on the developing pericarp of selected Rosa species (Rosaceae)

58%

Guzicka M., Zielinski J., Tomaszewski D., Gawlak M.

Dendrobiology

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2012

|

tom 68

Results of anatomical studies on the developing pericarp of selected wild roses are presented. Using SEM and CLSM, the changes in the pericarp structure of 5 species have been observed during its formation, from the flowering stage to fully ripe achenes. In the morphological development of the pericarp of Rosa species two main phases can be distinguished: the phase of intensive growth of the pericarp during which the fruit achieves its final shape and volume, and the subsequent phase of pericarp ripening when no significant morphological changes in the pericarp occur. Similarly, in the process of the anatomical development of the pericarp two phases are noticeable, however, during both stages, great internal changes proceed in the fruit. The first phase consists of intensive cell divisions and enlargement, gradual thickening of cell walls and formation of all pericarp layers. Due to these changes, the pericarp achieves its final anatomical structure. The second phase, involving the pericarp ripening, is manifested in the modification of cell walls, mainly by their quick thickening, but first of all by their lignification. The lignification of pericarp cell walls begins in the inner endocarp; it proceeds in the outer endocarp, later in mesocarp and finishes in the hypodermal cells of the exocarp. The epidermal cells remain alive the longest and their walls do not (or hardly) become lignified. The death of all cells finishes the pericarp ripening.

19

Histological and SEM studies on organogenesis in endosperm-derived callus of kiwifruit [Actinidia deliciosa cv. Hayward]

58%

Popielarska M., Slesak H., Goralski G.

Acta Biologica Cracoviensia. Series Botanica

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2006

|

tom 48

|

nr 2

Regeneration in endosperm-derived callus of Actinidla deliciosa cv. Hayward was documented by SEM combined with histology. Two kinds of callus, morphogenic and nonmorphogenic, were observed. Morphogenic callus consisted of compact cell clusters with epidermis-like tissue covered with a mucilaginous or continuous membranous layer, which partially disappeared, turned into fibrils, or became damaged. Regenerating shoots consisted of the apex and primordial leaves. Abnormal structures were also formed, frequently arrested in development. PAS reactions indicated that the mucilaginous layer and network present in intercellular spaces contains polysaccharides. Nonmorphogenic callus consisted of weakly attached cells without a covering membranous layer.

Ograniczanie wyników

Comparative scanning studies on cockerell (Gallus domesticus) spermatozoa with the use of SEM and AFM method

Hair types in Polish selected taxa of Potentilla subsect. Collinae [Rosaceae]

Comparative morphology and anatomy of the leaves of Ginkgo biloba L. cultivars

The SEM study on brush cells on surface of tongue in cat's embryos

Procedures for obtaining optimal SEM images of coccolithophore debris in coccolith limestones

Micromorphology of pollen grains of fruit trees of the genus Prunus

A new approach to air pollution determination using annual rings: dendro-chemical elemental analysis of annual rings by SEM-EDS

Micromorphology of stamens of some species of the genus Sansevieria petagna (Asparagaceae)

Sequences of epicuticular wax structures along stems in four selected tree species

New records of Dactylobiotus parthenogeneticus Bertolani, 1982 provide insight into its genetic variability and geographic distribution

Morphology of the first instar larvae of two species of Taxigramma Perris, 1852 (Diptera: Sarcophagidae: Miltogramminae)

A comparative LM and SEM study of the structure of the mucosal glands of the gallbladder in two species of canids: the dog and the Chinese raccoon dog

Meriderma species (Myxomycetes) from the Polish Carpathians: a taxonomic revision using SEM-visualized spore ornamentation

Pollen morphology of selected European species of the genus Allium L. (Alliaceae)

SEM studies on sporangia and pollen morphology of Gingko biloba cultivars

Micromorphology of the epidermis and anatomical structure of the leaves of Scorzonera hispanica L.

Orobanche elatior and O. kochii (Orobanchaceae) in Poland: distribution, taxonomy, plant communities and seed micromorphology

Anatomical study on the developing pericarp of selected Rosa species (Rosaceae)

Histological and SEM studies on organogenesis in endosperm-derived callus of kiwifruit [Actinidia deliciosa cv. Hayward]