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The effect of GA3 and the standard preservative on keeping qualities of cut LA hybrid lily ‘Richmond'

100%

Rabiza-Swider J., Skutnik E., Jedrzejuk A., Lukaszewska A., Lewandowska K.

Acta Scientiarum Polonorum. Hortorum Cultus

2015

tom 14

nr 4

The aim of the experiment was to evaluate effects of gibberellic acid and the standard preservative (composed of 8HQC and sucrose) on keeping qualities of cut lily flowering shoots (L. longiflorum × Asiatic hybrid, ‘Richmond’). These effects were tested in several experimental variants: the complete leafy shoot with the inflorescence (flowering shoot), inflorescence on a leafless shoot, decapitated leafy shoot, detached inflorescence and single leaves, in order to see how the components of a holding solution affect the particular plant organs on a lily flowering shoot. An experimental variant affected flower bud opening but less so the flower longevity. Keeping qualities such as vase life of lily flower and inflorescence, rate of bud opening and flower diameter were improved by the preservative only on a complete flowering shoot. Gibberellic acid as well the mixture of GA3 plus the standard preservative prolonged longevity of flowers in all the experimental variants. Gibberellic acid delayed leaf yellowing which was in turn hastened by the preservative except in leaves on decapitated shoots. Leaf senescence was the earliest in detached single leaves.

Effect of various preculture and osmotic dehydration conditions on cryopreservation efficiency and morphogenetic response of chrysanthemum shoot tips

88%

Kulus D., Serocka M., Mikula A.

Acta Scientiarum Polonorum. Hortorum Cultus

2018

tom 17

nr 1

The aim of this study was to evaluate the influence of sucrose concentration in the preculture medium, as well as the duration of osmotic dehydration on the efficiency of chrysanthemum ‘Richmond’, ‘Lady Orange’ and ‘Lady Salmon’ cryopreservation by encapsulation-dehydration technique. For all cultivars tested, the best regrowth of cryopreserved shot tips was recorded with 0.25 M sucrose concentration and 10 µM ABA during a two-week preculture, followed by a 4- or 7-day osmotic dehydration. The survival rate ranged from 56.8–58.0% (the Lady group) to 63.6% (‘Richmond’). However, the ability to grow was smaller and reached 18.2–50.7%. It was found that higher sucrose concentration during the preculture slowed the growth of chrysanthemum shoot tips and led to an increased formation of multiple shoots (by activating axillary buds) or deformed adventitious shoots (incapable of further growth). The frequency of tissue hyperhydricity also increased, while the rhizogenesis efficiency decreased when higher sucrose concentration in the preculture medium was applied. The influence of osmotic dehydration duration on the explants morphogenetic response was cultivar-dependent.

Ograniczanie wyników

2 Acta Scientiarum Polonorum. Hortorum Cultus

1 Jedrzejuk A.

1 Kulus D.

1 Lewandowska K.

1 Lukaszewska A.

1 Mikula A.

1 Rabiza-Swider J.

1 Serocka M.

1 Skutnik E.

1 2018

1 2015

Wyniki wyszukiwania

The effect of GA3 and the standard preservative on keeping qualities of cut LA hybrid lily ‘Richmond'

Effect of various preculture and osmotic dehydration conditions on cryopreservation efficiency and morphogenetic response of chrysanthemum shoot tips