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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Comparison of phenylethanoids content in Rhodiola kirilowii and Rhodiola rosea roots applying a newly developed UPLC-MS/MS method

100%
Gryszczynska A., Lowicki Z., Opala B., Krajewska-Patan A., Buchwald W., Czerny B., Mielcarek S., Mrozikiewicz P. M.
Herba Polonica
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2012
|
tom 58
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nr 4
A concentration of two phenylethanoids in the roots of two species: Rhodiola kirilowii and rosea were compared, aqueous and hydroalcoholic extracts from those plants were also analyzed. In order to determine the content of p-tyrosol and salidroside, the ultra performance liquid chromatography connected with a tandem mass spectrometry (UPLC-ESI MS/MS, Waters) was used. The obtained results shown that content of measured phenylethanoids depends on Rhodiola species. Roots of R. kirilowii contain more p-tyrosol, while R. rosea roots are reacher in salidroside. Our results indicate that the application of UPLC MS/MS method allows to determine the phenylethanoids content in tested samples with satisfactory precision.
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Determination of lotaustralin in Rhodiola species

86%
Gryszczynska A., Lowicki Z., Opala B., Krajewska-Patan A., Buchwald W., Czerny B., Mielcarek S., Mrozikiewicz P. M.
Herba Polonica
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2013
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tom 59
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nr 2
In our research, the concentration of lotaustralin in the roots of two species Rhodiola kirilowii and Rhodiola rosea were compared. Aqueous and hydroalcoholic extracts from those plants were analyzed too. To determine the content of this compound the ultra performance liquid chromatography – tandem mass spectrometry (UPLC-MS/MS, Waters) was used. The obtained results showed that the content of measured lotaustralin depends on the species of Rhodiola. R. rosea roots are the richer source of lotaustralin then R. kirilowii. The same situation was observed in the extracts. A hydroalcoholic extract from R. rosea contains up to 135.276 mg of lotaustralin in 100 g of dry powdered material. In the case of R. kirilowii extracts, an aqueous extract contained more lotaustralin (74.791 mg/100 g of dry powdered material) then a hydroalcoholic extract.
3
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Comparison of proanthocyanidins content in Rhodiola kirilowii and Rhodiola rosea roots - application of UPLC-MS/MS method

72%
Gryszczynska A., Krajewska-Patan A., Buchwald W., Czerny B., Mielcarek S., Rudzinska K., Mroziewicz P. M.
Herba Polonica
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2012
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tom 58
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nr 3
The purpose of presented study was the comparison of flavan-3-ol content in the roots of Rhodiola kirilowii and Rhodiola rosea with use of UPLC-MS/MS method. Two kinds of extract were prepared: aqueous extract and 50% v/v ethanol extract. The worked out UPLC MS/MS method allowed to determine the content of five flavan-3-ols: (+)-catechin, (-)-epicatechin, (-)-epigallocatechin, (-)-epicatechin gallate (ECG) and (-)-epigallocatechin gallate (EGCG). The obtained results shown that the content of measured catechins was higher in R. kirilowii roots than in R. rosea. Both Rhodiola roots contain EGCG as a main proanthocyanidin compound – the content in R. kirilowii roots is about 0.14%. Our results indicate that application of ultra performance liquid chromatograph connected to a tandem mass spectrometer (UPLC MS/MS method) allows to determine the proanthocyanidins content in tested samples with satisfactory precision and can be used in the Rhodiola sp. phytochemical investigations.
4

Zawartosc zwiazkow biologicznie czynnych w hodowlach kalusa i w hodowlach zawiesinowych Rhodiola kirilowii [Regel.] Maxim.

72%
Krajewska-Patan A., Furmanowa M., Dreger M., Lowicka A., Gorska-Paukszta M., Mscisz A., Mielcarek S., Przybylak J. K., Buchwald W., Mrozikiewicz P. M.
Herba Polonica
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2006
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tom 52
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nr 3
47-48
5
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Proanthocyanidins in Rhodiola kirilowii and Rhodiola rosea callus tissues and transformed roots - determination with UPLC-MS/MS method

72%
Gryszczynska A., Krajewska-Patan A., Dreger M., Buchwald W., Pietrosiuk A., Zych M., Karasiewicz M., Bogacz A., Kujawski R., Furmanowa M., Czerny B., Mielcarek S., Gazda P., Mrozikiewicz P. M.
Herba Polonica
|
2012
|
tom 58
|
nr 4
Several species of Rhodiola genus (Crassulaceae family) like Rhodiola kirilowii and Rhodiola rosea are used in official or traditional medicine. The aim of this study was to determine qualitative and quantitative content of proanthocyanidins using ultra performance liquid chromatograph connected to a tandem mass spectrometer (UPLC MS/MS method) in the callus tissues and in the transformed roots (infected by Agrobacterium rhizogenes LBA 9402 strain) of R. kirilowii and R. rosea. This validated assay allows to determine the content of five flavan-3-ols: (+)-catechin, (-)-epicatechin, (-)-epigallocatechin, (-)-epicatechin gallate (ECG), (-)-epigallocatechin gallate (EGCG). Our results concerning the material from in vitro cultivation of R. kirilowii and R. rosea indicate that R. rosea callus can be a better source of catechin when compared with other tested materials, especially when the content of (-)-gallate epigallocatechin is taken under consideration (3.429 mg/100 g of dry powdered material). The application of UPLC MS/MS method allowed to determine the content of proanthocyanidins in tested samples with satisfactory precision and can be used in the phytochemical investigations of Rhodiola sp. in vitro cultivated tissues.
6
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Determination of chlorogenic and gallic acids by UPLC-MS/MS

72%
Gryszczynska A., Opala B., Lowicki Z., Krajewska-Patan A., Buchwald W., Czerny B., Mielcarek S., Boron D., Bogacz A., Mrozikiewicz P. M.
Herba Polonica
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2013
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tom 59
|
nr 1
The aim of our study were qualitative and quantitative analyses of two polyphenolic acids: chlorogenic and gallic acids. These compounds were determined in two species of Rhodiola: R. kirilowii and R. rosea. After collecting plants, aqueous and hydroalcoholic extracts were prepared. In order to identify analysed polyphenolic compounds ultra performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS, Waters) was used. Gallic acid is commonly found in the roots of these plants. Aqueous extract in both species is a rich source of gallic acid. The UPLC-MS/MS studies allow to use this analytical method for determination of polyphenolic acids accordance with the requirements of ICH. Chromatographic method developed by our team is more precise then previously published.
7
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Novel method of detection of phenylpropanoids of Rhodiola roots species

72%
Gryszczynska A., Opala B., Krajewska-Patan A., Lowicki Z., Buchwald W., Mielcarek S., Bogacz A., Karasiewicz M., Boron D., Czerny B., Mrozikiewicz P. M.
Herba Polonica
|
2013
|
tom 59
|
nr 1
The aim of the study was the identification and quantitative analysis of phenylpropanoid compounds in the roots of Rhodiola species. Rosavin, rosarin and rosin were determined in the roots of R. kirilowii and R. rosea from the field cultivation, Institute of Natural Fibres and Medicinal Plants. For the quantitative analysis, the ultra performance liquid chromatography - tandem mass spectrometry (UPLC-ESI MS/MS, Waters) was used. The results showed differences in the quantitative and qualitative assessments of these two species. In the root of R. kirilowii the presence of phenylpropanoids was not confirmed. In R. rosea the most common phenylpropanoid was rosavin (0.022%). The UPLC-MS/MS studies allowed to use this analytical method for determination of phenylpropanoids in the accordance with the requirements of ICH.
8

Wybrane aspekty aktywnosci behawioralnej wyciagow z Rhodiola kirilowii [Regel.] Maxim.

72%
Mikolajczak P. L., Okulicz-Kozaryn I., Kaminska E., Szulc M., Bobkiewicz-Kozlowska T., Krajewska-Patan A., Lowicka A., Mscisz A., Formanowa M., Mrozikiewicz P. M.
Herba Polonica
|
2006
|
tom 52
|
nr 3
68-69
9

Ocena dzialania przeciwzapalnego wyciagow z Rhodiola kirilowii [Regel.] Maxim. i Rhodiola rosea L.

58%
Okulicz-Kozaryn I., Mikolajczak P. L., Siekierkowska A., Bobkiewicz-Kozlowska T., Krajewska-Patan A., Lowicka A., Mscisz A., Furmanowa M., Mrozikiewicz P. M.
Herba Polonica
|
2006
|
tom 52
|
nr 3
56-57
10

Badania wybranych aspektow aktywnosci biologicznej wyciagow z tkanek kalusowych Rhodiola sp.

58%
Krajewska-Patan A., Furmanowa M., Mikolajczak P. L., Kedzia B., Mscisz A., Lowicka A., Dreger M., Gorska-Paukszta M., Buchwald W., Mrozikiewicz P. M.
Herba Polonica
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2006
|
tom 52
|
nr 3
50-51
11

Callus tissues of Rhodiola kirilowii [Regel] Maxim. - dynamic of growth and active compounds production

58%
Krajewska-Patan A., Dreger M., Buchwald W., Gorska-Paukszta M., Mielcarek S., Baraniak M., Mscisz A., Furmanowa M., Mrozikiewicz P. M.
Herba Polonica
|
2009
|
tom 55
|
nr 3
222-230
Rhodiola Kirilowii (Regel) Maxim. (Crassulaceae) is a traditional medicinal plant used in North Asia and China, especially in the cardiopulmonary disorders in the hypoxic conditions induced by high altitude. The presented results are the part of the investigations carried out in the Branch of Medicinal Plants of the Institute of Natural Fibres and Medicinal Plants in cooperation with the Department of Biology and Pharmaceutical Botany, Medical University in Warsaw on R. Kirilowii plants and tissue cultures. The aim of recent study was to determine the growth dynamics and active compounds production during the cultivation of callus tissues of R. Kirilowii on solid/liquid media. Tissue cultures of R. Kirilowii shown the ability to produce all the active compounds determined in the roots of plants of Polish origin. It is worth emphasizing, that rosavins, according to known literature, were not detected in roots of plants growing in Asia. The best time for collection the tissues from solid medium was fifth or sixth week of the culture – the tissues were growing dynamically and the contents of the main active compounds was high. The material from suspension should be collected in 12–15 days after inoculation. The obtained results will be applied in future investigations on the use of R. Kirilowii extracts in the experimental hypoxia in rats.
12

Badania nad toksycznoscia oraz dzialaniem adaptogennym i przeciwdrobnoustrojowym wyciagow otrzymanych z podziemnych czesci wybranych gatunkow Rhodiola L.

58%
Kedzia B., Furmanowa M., Krajewska-Patan A., Holderna-Kedzia E., Mscisz A., Wojcik J., Buchwald W., Mrozikiewicz P. M.
Herba Polonica
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2006
|
tom 52
|
nr 4
117-132
Badania toksykologiczne wyciągu z podziemnych części Rhodlola rosea wskazują na jego niską toksyczność ostrą i brak szkodliwego oddziaływania przy długotrwałym podawaniu zwierzętom doświadczalnym. Wyciągi z Rhodiola rosea, R. Kirilowii i R. quadrißda cechują się wyraźnym działaniem adaptogennym oraz szerokim spektrum działania przeciwdrob- no ustroj owego. Można je więc wykorzystać do wytwarzania preparatów podwyższających odporność nieswoistą, a także sprawność fizyczną i umysłową organizmu. Poza tym można je stosować do leczenia schorzeń dermatologicznych o etiologii drobnoustrojowej.
13

Badania toksykologiczne, farmakologiczne i mikrobiologiczne wyciagow z klaczy Rhodiola rosea, R. kirilowii i R. quadrifida

58%
Kedzia B., Formanowa M., Krajewska-Patan A., Holderna-Kedzia E., Mscisz A., Wojcik J., Buchwald W., Mrozikiewicz P. M.
Herba Polonica
|
2006
|
tom 52
|
nr 3
105-106
14

The effect of Chinese medicinal herb Rhodiola kirilowii extracts on cellular immunity in mice and rats

58%
Wojcik R., Siwicki A. K., Skopinska-Rozewska E., Wasiutynski A., Sommer E., Furmanowa M.
Polish Journal of Veterinary Sciences
|
2009
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tom 12
|
nr 3
Rhodiola kirilowii (RK) roots and rhizomes are traditionally used in China as a tonic, adaptogen, antimicrobial and anti-inflammatory drug. The aim of this work was to study the in vivo and in vitro effects of aqueous and 50% hydro-alcoholic extracts of RK rhizomes on some parameters of cellular immunity in H-2d mice and rats. We show for the first time that in vitro both extracts stimulated granulocyte activity and increased lymphocyte response to mitogens, and in vivo they enhanced the ability of lymphocytes derived from parental strain mice fed R. kirilowii aqueous and hydro-alcoholic extracts, to induce local cutaneous graft-versus-host reaction (GVH) in F1 hybrids. Conclusion: Rhodiola kirilowii extracts are cellular immunity enhancers.
15

Tissue culture of Rhodiola kirilowii [Regel.] Maxim - contents of biologically active compounds at different stages of growth

58%
Krajewska-Patan A., Furmanowa M., Mscisz A., Dreger M., Lowicka A., Gorska-Paukszta M., Mielcarek S., Mrozikiewicz P. M.
Herba Polonica
|
2006
|
tom 52
|
nr 4
The callus tissue obtained from Rhodiola Kirilowii (Regel.) Maxim was tested for growth dynamics and contents of active compounds. Biologically active compounds in the callus tissue were identified as salidroside, tyrosol, gallic acid, chlorogenic acid, caffeic acid and tannins. The lotaustralin was not detected either in the callus, or roots of R. Kirilowii
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