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Micropropagation of Rhodiola Kirilowii plants using encapsulated axillary buds and callus

100%

Zych M., Furmanowa M., Krajewska-Patan A., Lowicka A., Dreger M., Mendlewska S.

Acta Biologica Cracoviensia. Series Botanica

2005

tom 47

nr 2

This study assessed regrowth of micropropagated Rhodiola Kirilowii buds after encapsulation of axillary buds and differentiating callus in calcium alginate hydrogel and low-temperature preservation. This method of micropropagation was applied to obtain enough plant material for studies on chemical compounds of Rhodiola Kirilowii, a plant difficult to obtain from its natural environment. Axillary buds and differentiating callus were encapsulated in calcium alginate and stored at 4°C for 1 to 15 weeks and then transferred to hormone-free MS medium. The best results were obtained after six weeks of preservation: 100% of the encapsulated explants survived and developed into shoots and plantlets after subculture on basal MS medium. Longer storage of encapsulated axillary shoot buds decreased their regrowth, but the duration of storage of encapsulated differentiating callus had no significant influence on its survival.

Preliminary report on phytochemistry of medicinal plant Rhodiola Kirilowii [Regel.] Maxim

80%

Pietrosiuk A., Zych M., Kozlowski J., Furmanowa M.

Herba Polonica

2002

tom 48

nr 3

Extracts of Rhodiola Kirilowii plant roots (several years old plant) were studied by HPLC methods. The aim of these studies was searching for active substances: salidroside, tyrosol, rosavin, cinnamyl alcohol and triandrine. In studied extracts mentioned above substances were not found. On chromatograms only one peak (Rt 8,9 min., maximal absorbance at X =220 nm) of notidentified substance was observed. Further investigations are in progress.

Ograniczanie wyników

1 Acta Biologica Cracoviensia. Series Botanica

1 Herba Polonica

2 Furmanowa M.

2 Zych M.

1 Dreger M.

1 Kozlowski J.

1 Krajewska-Patan A.

1 Lowicka A.

1 Mendlewska S.

1 Pietrosiuk A.

1 2005

1 2002

Wyniki wyszukiwania

Micropropagation of Rhodiola Kirilowii plants using encapsulated axillary buds and callus

Preliminary report on phytochemistry of medicinal plant Rhodiola Kirilowii [Regel.] Maxim