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Chemical characterization of effective and ineffective strains of Rhizobium leguminosarum bv.viciae

100%

Izmailov S. F., Zhiznevskaya G. Y., Kosenko L. V., Troitskaya G. N., Kudryavtseva N. N., Borodenko L. I., Dubrovo P. N., Russa R., Pietras H., Lorkiewicz Z.

Acta Biochimica Polonica

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1999

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tom 46

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nr 4

Chemical composition of lipopolysaccharide (LPS) isolated from an effective (97) and ineffective (87) strains of R. l. viciae has been determined. LPS preparations from the two strains contained: glucose, galactose, mannose, fucose, arabinose, heptose, glucosamine, galactosamine, quinovosamine, and 3-N-methyl-3,6-dideoxyhexose, as well as glucuronic, galacturonic and 3-deoxyoctulosonic acid. The following fatty acids were identified: 3-OH 14:0, 3-OH 15:0, 3-OH 16:0, 3-OH 18:0 and 27-OH 28:0. The ratio of 3-OH 14:0 to other major fatty acids in LPS 87 was higher that in LPS 97. SDS/PAGE profiles of LPS indicated that, in lipopolysaccharides, relative content of S form LPS I to that of lower molecular mass (LPS II) was much higher in the effective strain 97 than in 87. All types of polysaccharides exo-, capsular-, lipo, (EPS, CPS, LPS, respectively) examined possessed the ability to bind faba bean lectin. The degree of affinity of the host lectin to LPS 87 was half that to LPS 97. Fatty acids (FA) composition from bacteroids and peribacteroid membrane (PBM) was determined. Palmitic, stearic and hexadecenoic acids were common components found in both strains. There was a high content of unsaturated fatty acids in bacteroids as well as in PBM lipids. The unsaturation index in the PBM formed by strain 87 was lower than in the case of strain 97. Higher ratio of 16:0 to 18:1 fatty acids was characteristic for PMB of the ineffective strain.

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Plonowanie i elementy struktury plonu bobiku w zaleznosci od szczepow Rhizobium leguminosarum var. viciae

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Dziamba S., Koput R., Lorkiewicz Z., Kowalczuk E.

Bibliotheca Fragmenta Agronomica

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2000

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tom 08

87-90

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A field experiment on competition of Rhizobium leguminosarum bv.viciae

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Lorkiewicz Z., Kowalczuk E.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1996

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tom 44

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nr 3-4

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Oddzialywanie zapraw chemicznych na przezywalnosc bakterii brodawkowych na nasionach bobiku oraz na proces symbiozy

63%

Martyniuk M., Oron J., Wozniakowska A., Martyniuk S.

Pamiętnik Puławski

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2000

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tom 121

41-47

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Survival of Rhizobia in two soils as influenced by storage conditions

63%

Martyniuk S., Oron J.

Polish Journal of Microbiology

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2008

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tom 57

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nr 3

Two soils were kept moist at 4°C, -20°C or air-dried at 20-22°C and after one week, one month, two months and six months of storage at these conditions changes in soil populations of Rhizobium leguminosarum bv. trifolii (Rlt) and Rhizobium leguminosarum bv. \iciae (Rlv) were examined. In one air-dried soil (from Grabów) markedly lower numbers of both Rlt and Rlv., as compared to the refrigerated or frozen samples, were found already after 1 week of storage. In the case of the second soil (from Osiny) air-drying significantly reduced numbers of the rhizobia after 2 and 6 months of storage. The soil from Osiny contained higher amounts of C org, total N and clay than the Grabów soil. Both soils stored moist in a refrigerator (4°C) or frozen (-20°C) retained similar populations of the examined rhizobia throughout the entire storage period, indicating that soil freezing is not detrimental for the examined rhizobia.

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Reakcja szczepów Rhizobium leguminosarum bv. viciae oraz Bradyrhizobium sp.[lupini] na ph w hodowlach na pożywkach sztucznych

62%

Kucharski J., Kuczynska-Krogulec L.

Acta Academiae Agriculturae ac Technicae Olstenensis. Agricultura

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1996

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tom 63

59-66

Z brodawek bobiku, peluszki i łubinu uprawianych na glebie o zróżnicowanym pH (od 4.5 do 7.0) wyizolowano bakterie symbiotyczne i w doświadczeniu laboratoryjnym badano wpływ pH 4.0; 5.0; 6.0 i 7.0 na namnażanie się Bradyrhizobium sp. (lupini) i Rhizobium leguminosarum bv. viciae oraz na aktywność dehydrogenaz. Prześledzono także wpływ wapnowania gleby na liczebność bakterii oligotroficznych, makrotroficznych, amonifikatorów, zbiałczających azot i Azotobacter oraz promieniowców i grzybów. W wyniku badań stwierdzono, że dla szczepów Rhizobium leguminosarum bv.viciae (współżyjących z bobikiem i peluszką) optymalnym było pH 7.0, a dla Bradyrhizobium sp. (lupini) - 6.0, przy czym ten ostatni gatunek dobrze znosił zakwaszenie podłoża nawet do pH 4.0. Największą aktywnością dehydrogenaz cechowały się komórki Rhizobium i Bradyrhizobium hodowane na pożywkach o pH 7.0. Wapnowanie stymulowało namnażanie się bakterii oligotroficznych, makrotroficznych, zbiałczających azot i amonifikatorów w glebie spod wszystkich roślin oraz zbiałczających azot i promieniowców w glebie spod peluszki i łubinu. Zabieg ten działał inhibicyjnie na grzyby glebowe oraz na zdolność namnażania się in vitro bakterii Rhizobium współżyjących z bobikiem.

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Localization of expansin-like protein in apoplast of pea [Pisum sativum L.] root nodules during interaction with Rhizobium leguminosarum bv. viciae 248

51%

Sujkowska M., Borucki W., Golinowski W.

Acta Societatis Botanicorum Poloniae

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2007

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tom 76

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nr 1

During nodule development on pea roots, apoplast undergoes changes in activity of plant cell wall proteins such as expansins (EXPs). Because the accumulation of EXP protein has been correlated with the growth of various plant organs, we investigated using Western Blot and immunolocalization studies with antibody against PsEXP1, whether this protein was accumulated in the expanding cells of nodule. Immunoblot results indicated the presence of a 30-kDa band specific for pea root nodules. The EXP proteins content rose during growth of pea root nodules. Expansin(s) protein was localized in nodule apoplast as well as in the infection thread walls. The enhanced amount of expansin-like proteins in meristematic part of nodule, root and shoot was shown. The localization of this protein in the meristematic cell walls can be related to the loosening of plant cell wall before cell enlargement. Both, plant cell enlargement and infection thread growth require activity of expansin(s). Possible involvement of EXPs in the process of pea root nodule development is also discussed.

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Three-year studies on competition among Rhizobium leguminosarum bv.viciae for nodulation in Polish and Ukrainian soil

51%

Kowalczuk E., Lis N., Lorkiewicz Z.

Acta Microbiologica Polonica

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1997

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