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Structural analyses of AC4 protein of Sri Lankan cassava mosaic virus

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Gupta S., Ganguli S., Basu P., Datta A.

International Letters of Natural Sciences

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2015

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tom 06

RNA silencing is one of the important phenomenon in plant defense mechanism, it actively protect host plants against viral infections. Existing viruses must have developed counter defense strategies to survive this arms race. Such counter defense strategy is the viral silencing suppressor (VSRs) which have been reported to directly interfere with the various steps leading to the interference of viral RNAs. Most identified VSRs are multifunctional, besides being RNA-silencing suppressors, they often perform essential roles by functioning as coat proteins, helper components for viral transmission, replicases and movement proteins, proteases or transcriptional regulators. One such identified VSR is AC4 of Sri Lankan cassava mosaic virus strain. Trivial knowledge about the structure –function relationship of this VSR leads to this work, where we focus on the structure generation by modelling to identify the mode of interactions with the various effector molecules of the silencing pathways. Structural analyses have been performed to screen interacting residues. Results indicate conserved structural features which signify propensity of functional interactions and further shows that this VSR can be a potent tool for the analysis of RNA silencing mechanisms and the relationships between different silencing pathways and VSRs.

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Optimization of transient Agrobacterium-mediated gene expression system in leaves of Nicotiana benthamiana

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Wydro M., Kozubek E., Lehmann P.

Acta Biochimica Polonica

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2006

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tom 53

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nr 2

Here we report on a simple and reproducible system of Agrobacterium-mediated transient gene expression assay that utilizes infiltration of young Nicotiana benthamiana leaves. Although some of the phenomena described in this paper have been already reported by other researchers, here we have further developed them. The highest level of transient gfp gene expression was detected in the youngest leaves of N. benthamiana infiltrated with A. tumefaciens strains AGL0 and EHA105 precultured in the presence of 450-600 µM acetosyringone. Although the maximum level of transient gfp gene expression was restricted presumably by RNA silencing, it was completely suppressed in the presence of the viral protein HC-Pro. The transient expression system described here can be used to identify new viral suppressors of RNA silencing, for detailed analysis of unidentified genes and for industrial production of proteins in plants as well.

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Beyond sequence similarity – the curious case of GW/WG protein domain

80%

Karlowski W. M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

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2011

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tom 92

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nr 4

Ograniczanie wyników

1 Acta Biochimica Polonica

1 BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

1 International Letters of Natural Sciences

1 Basu P.

1 Datta A.

1 Ganguli S.

1 Gupta S.

1 Karlowski W. M.

1 Kozubek E.

1 Lehmann P.

1 Wydro M.

1 2015

1 2011

1 2006

Structural analyses of AC4 protein of Sri Lankan cassava mosaic virus

Optimization of transient Agrobacterium-mediated gene expression system in leaves of Nicotiana benthamiana

Beyond sequence similarity – the curious case of GW/WG protein domain