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1

A novel RFLP - AluI polymorphism of the bovine calpastatin [CAST] gene and its association with selected traits of beef

100%
Juszczuk-Kubiak E., Rosochacki S. J., Wicinska K., Szreder T., Sakowski T.
Animal Science Papers and Reports
|
2004
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tom 22
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nr 2
Calpastatin (CAST) is a specific inhibitor of the ubiquitous calcium-dependent proteases – μ-calpain and m-calpain, found in mammalian tissues. The level of components included into the calpain-calpastatin system determine the rate of post mortem tenderization of meat.In the coding region of the bovine CAST gene (CAST) the new nucleotide sequence polymorphism was found being a substitution G→C at position 61 nt within the exon 1C (consensus sequence – GenBank AF117813). This sequence fragment of SNP position has already been deposited in the GenBank database under accession no. AY258325. Consensus of bovine CAST sequence with that of human (GenBank M86257 and M28230) revealed that G→C substitution was located at position 1460 nt of exon 12. Computer analysis of the mutation showed the Ser→Thr substitution at position 20 of amino acid sequence of CAST protein. The mutation creates a new AluI restriction site and,therefore, can be easily detected with PCR-RFLP.The CAST RFLP-AluI polymorphism was studied in 138 bulls of seven breeds, including the native Polish Red (PR, preserved), and Polish Black-and-White (BW) breed. The frequency of alleles C and G varied between the breeds considered, the mean reaching 0.69 and 0.31, respectively. No homozygous genotype GG was found in Red Angus, Charolaise and Hereford bulls.
2

Cryptosporidium identification in HIV-Infected Humans. Experience from Kinshasa, the Democratic Republic of Congo

72%
Wumba R. D., Zanga J., Mbanzulu K. M., Mandina M. N., Kahindo A. K., Aloni M. N., Ekila M. B.
Acta Parasitologica
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2015
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tom 60
|
nr 4
3

Possibilities in identification of genomic species of Burkholderia cepacia complex by PCR and RFLP

72%
Navratilova L., Chroma M., Hanulik V., Raclavsky V.
Polish Journal of Microbiology
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2013
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tom 62
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nr 4
The strains belonging to Burkholderia cepacia complex are important opportunistic pathogens in immunocompromised patients and cause serious diseases. It is possible to obtain isolates from soil, water, plants and human samples. Taxonomy of this group is difficult. Burkholderia cepacia complex consists of seventeen genomic species and the genetic scheme is based on recA gene. Commonly, first five genomovars occurre in humans, mostly genomovars II and III, subdivision IIIA. Within this study we tested identification of first five genomovars by PCR with following melting analysis and RFLP. The experiments were targeted on eubacterial 16S rDNA and specific gene recA, which allowed identification of all five genomovars. RecA gene appeared as more suitable than 16S rDNA, which enabled direct identification of only genomovars II and V; genomovars I, III and IV were similar within 16S rDNA sequence.
4
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Molecular characterization of ‘Candidatus Phytoplasma mali’ strains from Bulgaria and Poland

72%
Cieslinska M., Borisova A.
Acta Scientiarum Polonorum. Hortorum Cultus
|
2019
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tom 18
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nr 5
During 2015, samples from 22 apple trees showing proliferation symptoms were collected in southwest Bulgaria and Central and South Poland and tested for phytoplasma presence. ‘Candidatus Phytoplasma mali’ was identified in 18 samples based on results of restriction fragment length polymorphism (RFLP) analysis of the 16S rRNA gene amplified in nested PCR using primer pair P1/P7 followed by R16F2n/R16R2 and F1/B6 primer pairs. The nitroreductase and rhodonase-like genes and ribosomal protein genes rpl22 and rps3 were then analyzed using PCR-RFLP technique to study the genetic variability of the phytoplasma strains. Two restriction profiles, P-I or P-II, were obtained from fragments of 16S rDNA plus 16S-23S spacer region digested with HpaII enzyme. Restriction fragment length polymorphism analysis of nitroreductase and rhodonase-like genes using digestion with HincII endonuclease revealed that all ‘Ca. P. mali’ strains belonged to the subtype AP-15. Analysis of rpl22 and rps3 ribosomal protein genes digested with AluI enzyme resulted in classification of detected phytoplasma strains to rpX-A subgroup.
5

Use and comparison of molecular and classical methods for the identification of moulds isolated from the soil

72%
Piegza M., Rzasa J., Siepka E., Witkowska D.
Acta Scientiarum Polonorum. Biotechnologia
|
2010
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tom 09
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nr 4
Present work contains the attempt to compare methods of microorganisms identification, for example, the similarity between species – molecular and classical. The both methods were used because classical approaches based on the use of morphological criteria are, as in several other fungi, difficult to apply in Trichoderma, due to plasticity of characters to interpret. Consequently, almost all recent studies have used molecular data to characterize and identify species. The comparison of these two techniques is based on using guidelines of macroscopic and microscopic evaluation of few classical factors as well as RAPD and RFLP – molecular techniques. Results indicate that none of the technique being compared is adequate when used alone, although molecular ones are more accurate. There is no complete convergence between RAPD and RFLP what makes it even harder to interpret but this fact can be the result of inconvenient markers used in experiment.
6
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Content available

Detectability of tick-borne agents DNA in the blood of dogs, undergoing treatment for borreliosis

72%
Wodecka B., Rymaszewska A., Sawczuk M., Skotarczak B.
Annals of Agricultural and Environmental Medicine
|
2009
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tom 16
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nr 1
9-14
7

Association between the growth hormone combined genotypes and dairy traits in Polish Black-and-White cows

72%
Dybus A., Grzesiak W., Szatkowska I., Blaszczyk P.
Animal Science Papers and Reports
|
2004
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tom 22
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nr 2
Associations were analysed between the bovine growth hormone combined genotypes (GH/AluI-MspI) and milk production traits in a total of 900 Polish Black-and-White (Polish Friesian) cows. PCR-RFLP method was used for genotyping. The following were frequencies of GH/AluI-MspI: 0.463 LL/++, 0.276 LV/++, 0.157 LL/+-, 0.059 LV/+-, 0.025 VV/++, 0.019 LL/-- and 0.001 (only 1 observation) of VV/+-.Significant differences were found in analysed dairy traits between cows of different GH/AluI-MspI combined genotypes. It is difficult, however, to trace a defined trend in all lactations, what to some extent complicates the inference. It is concluded that introducing the information on GH/AluI-MspI into dairy cattle marker-assisted selection (MAS) programmes would be risky.
8

Simultaneous detection of malignant hyperthermia and genetic predisposition for improved litter size in pigs by multiplex PCR-RFLP

72%
Omelka R., Vasicek D., Martiniakova M., Bulla J., Bauerova M.
Folia Biologica
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2004
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tom 52
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nr 1-2
9

Identification by PCR-RFLP of a fungus isolated from mycorrhizal roots of a distinguishable birch growing in areas disturbed by industry

72%
Krupa P.
Polish Journal of Environmental Studies
|
1999
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tom 08
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nr 3
10

Characterization of Fusarium isolates from rice, sugarcane and maize using RFLP-IGS

58%
Hsuan H. M., Zakaria L., Salleh B.
Journal of Plant Protection Research
|
2010
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tom 50
|
nr 4
Isolates of Fusarium from rice, sugarcane and maize were identified as F. verticillioides, F. sacchari, F. proliferatum F. subglutinans, F. fujikuroi and F. oxysporum. The species were then characterized by restriction analysis of intergenic spacer (RFLP-IGS) using AluI, Eco88I, RsaI and XhoI. Twenty-five haplotypes were identified among the isolates of Fusarium which indicated high levels of variations. UPGMA cluster analysis was conducted to cluster the isolates and to estimate the intraspecific and interspecific variability. Isolates of F. fujikuroi from rice were clustered together with isolates of F. proliferatum from rice and maize with a similarity value of 88–100%. Isolates of F. verticilliodes from maize and sugarcane were clustered together with a similarity value ranging from 92–100%, and two isolates from rice formed another cluster. Isolates of F. oxysporum from maize and rice were clustered together with a similarity value ranging from 87–95%. Isolates of F. subglutinans from rice and maize, and F. sacchari from rice and sugarcane were also clustered together with a similarity value of 77–100%. Based on RFLP-IGS analysis, variability was observed within and between species of Fusarium from rice, maize and sugarcane and the technique could be use to complement morphological characterization and to determine genetic relationships between the species.
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