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The total of 35 splendid pear trees was inventoried in the Citadel Park and they constitute approximately 13% of the most valuable trees in this park. The circumferences of the measured trees ranged from 150 to 264 cm. Considerable circumferences were determined in the case of 32 pear trees, of which 11 trees had monumental circumferences and 21 specimens – close to monumental circumferences. From among 14 trees found to be in a very good health condition and having monumental or close to monumental circumferences, “candidates” for legal protection in the form of nature monuments should be selected.
Z kwiatów gruszy dziko rosnącej za pomocą chromatografii kolumnowej wyizolowano kwas ursolowy. Jego strukturę potwierdzono metodami spektralnymi (IR, NMR). Inne triterpeny i sterole badano metodą TLC, GC, GC/MS we frakcjach otrzymanych z wyciągu benzynowego i chloroformowego z kwiatów gruszy. Z triterpenów zidentyfikowano kwas oleanolowy, ursolowy, α-amyrynę, neooleanadien; ze steroli β-sitosterol i sacharostenon. Porównano chromatograficznie (TLC) zespół steroli i triterpenów występujących w kwiatach gruszy dzikiej z zespołem steroli i triterpenów w kwiatach wybranych odmian hodowlanych. W badanych surowcach zespół wyżej wymienionych związków był jakościowo identyczny.
RAPD and ISSR techniques were used in identification of 26 pear cultivars. As a result of reactions carried out with RAPD 25 primers, 103 polymorphic DNA frag­ments were obtained. The largest number of polymorphic dNa fragments (7-8) was produced in reactions with the following primers: OPT 15, OPG 16 and OPG 19. Identification of cultivars and rootstocks was achievable with the use of OPT 15, OPG 19 and OPU 07 primers. In the reactions performed with 22 ISSR primers, 135 markers of pear cultivars were obtained. The size of fragments varied from 280 to 1790 bp. The greatest number of polymorphic products (9) was obtained in the reac­tions with primers: 830, 840 and 844. Primers 840 and 844 enabled identification of all tested genotypes. Degree of DNA polymorphism was estimated at 56.3% (RAPD) and 71.5% (ISSR). Results of the research confirm the usefulness of both techniques in identifying pear cultivars.
Celem pracy było określenie wpływu procesu odwadniania osmotycznego na zawartość związków polifenolowych w suszach gruszkowych. Materiałem do badań były owoce grusz azjatyckich (Pyrus pyrifolia Nakai) odmian Hosui i Shinseiki oraz owoce gruszy europejskiej (Pyrus communis L.) odmiany Konferencja. Kostki gruszek o boku 10 mm odwadniano osmotycznie w roztworach sacharozy, koncentratu gruszkowego i mieszaninie koncentratu gruszkowego i roztworu sacharozy (1:1). Proces odwadniania prowadzono w temp. 20°C przez 1, 3 i 6 godz. Następnie owoce po odwadnianiu suszono konwekcyjnie w temp. 70ºC przez 4,5 godz. W owocach świeżych, po zabiegach wstępnych oraz w otrzymanych suszach oznaczono zawartość polifenoli ogółem oraz zawartość poszczególnych związków fenolowych metodą HPLC. Odwadnianie osmotyczne wpłynęło na zawartość polifenoli ogółem w próbkach gruszek. Stwierdzono, że odwadnianie w roztworze koncentratu gruszkowego, jak i w mieszaninie koncentratu i sacharozy spowodowało wzrost zawartości polifenoli. Inaczej oddziaływał roztwór sacharozy, powodując zmniejszenie zawartości polifenoli, tym większe im dłużej trwał proces odwadniania osmotycznego. Po 6 godz. procesu w roztworze sacharozy zawartość polifenoli w kostkach była o 50% mniejsza niż w surowcach. Proces suszenia konwekcyjnego spowodował zmniejszenie zawartości polifenoli ogółem w suszach w stosunku do materiału odwadnianego.
The paper describes a new taxon: Pyrus × myloslavensis, i.e. a spontaneous hybrid between P. communis (Common Pear) and P. salicifolia (Willowleaf Pear), found near Miłosław town (Wielkopolska province, West Poland). No such hybrid has been reported in the literature so far. P. × myloslavensis has elliptic leaves, 1.1–7.6 cm long and 1.0–2.9 cm wide, margin entire, near the apex shallowly and remotely serrate, shortly acute, silvery tomentose on both sides. Fruit of the hybrid is relatively large (mean weight 56.2 g), green-yellow, without rust-coloured patches and blush.
Most fruit-tree species, including pear (Pyrus) have genetically controlled ability to outcrossing (self-incompatibility). From the orchard practice point of view outcrossing is desired for obtaining acceptable yield level. So, the aim of the present study was to learn problem of self-incompatibility or cross-compatibility of six pear (P. communis) cultivars (Amfora, Radana, Red Williams, Carola, Conference, Dicolor) and wild ecotype of P. pyraster and the evaluation of investigated cultivars as good polinators for each other. The mode of pollination consists of intracultivar self- and cross-pollination and intercultivar diallel cross-pollination. The pollination was done in three different environmental conditions i.e. orchard, glasshouse and laboratory. Self-incompatibility or cross-compatibility of investigated cultivars was evaluated on the base of the pollen germination index (PGI). Six cultivars of pear and one ecotype of wild pear which were under investigation showed full self-incompatibility. In some inter-cultivar and interspecific pollinations full cross-incompatibility or unilateral incompatibility was observed.
The aim of this work is to produce the generative rootstocks for the wild pears, which will be of moderate denseness. The initial material is the population of wild pear in the area of Polimlje. The study focused on few segments. Very first one included recording of the phenological traits – first flowering, full flowering, end of flowering and harvest period. The other segment comprised pomological features, i.e. physical [fruit weight (g), fruit size (mm), mass of dry seed (g), and number of seed in 1 kg of the fruit]. Seeds from 9 selected genotypes of wild pears were planted in the nursery and raised seedlings were evaluated for nursery characteristics: germination, seedling vigor, uniformidty and branching. Raised seedlings were used as rootstocks for scion cultivar ‘Grand Champion’. The most important benefit of this study is the fact that the parent trees, the seeds of which provide the best morphological and physiological characteristics for the production of generative rootstocks, were found and favored (in situ). Results of this research show that the rapid growth and uniformity of scions depend on the genetic characteristics of generative rootstocks of selected genotypes of wild pears, such as: plant height, stem diameter (corpulence), branching and uniformity. The selected genotypes, especially ‘Genotype 11’, proved to be the best for mountainous areas of the north of Montenegro, higher altitudes and poorer types of soil. The ‘Genotype 11’ is suggested as the best option for the production of generative rootstocks due to its small vigour level.
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Peroxidase (EC 1.11.1.7; donor: hydrogen-peroxide oxidoreductase, POD) is one of the key enzymes controlling plant growth, differentiation and development. The enzyme participates in construction, rigidification and eventual lignification of cell walls, biosynthesis of ethylene from 1-aminocyclopropane-1-carboxylic acid and H2O2, regulation of auxin level through auxin catabolism, protection of tissue from damage and infection by pathogenic microorganisms, the oxidation of indoleacetic acid. For peroxidase activity in wild pears extract one pH optimum was observed at 6.5 that probably belong to atleast one isoenzyme. Activity of peroxidase in presence of guaiacol and H2O2 was optimum after incubation at 40 °C. Maximum activity of peroxidase is 300 % .Activity increased to 240 %, 300 %, 70 % and 10 % after 60 minute incubation at 30, 40, 45 and 60 °C for peroxidase. Incubation at high temperature (70 °C) was accompanied with decrease of activity to 10 % peroxidase activity.
Interspecific compatibility and self-compatibility in Pyrus communis, P. pyraster and P. salicifolia were evaluated. Degree of compatibility was determined by means of fluorescence microscope. Self-incompatibility evaluated on the basis of the pollen tubes growth showed that two cultivars of P. communis (Bera Hardy and Lukasówka) were self-compatible and the other three cultivars were self-incompatible. All ecotypes of P. pyraster are self-incompatible. In interspecific crosses full inter-incompatibility or unilateral self-incompatibility was observed.
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In the present study, the effects of intermittent microwave-vacuum drying on the “Deveci” pear in terms of the drying kinetics, mineral content, protein content, rehydration ratio, color, energy, specific energy and microstructure were investigated. According to the drying treatments, increased microwave power (100 and 200 W) and vacuum (200 and 400 mmHg) applications provided higher drying rates and protein content. Dried pear samples had a higher mineral content than fresh samples because of the increasing dry matter content. At the higher vacuum level experiments, the energy consumption reduced and the rehydration ratio increased. Moreover, the higher microwave power caused a decrease of the L* (lightness) value. However, a regular pore size and pore distribution in all drying experiments were observed in the microstructures of microwave-vacuum-dried samples.
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