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Pyrenophora tritici-repentis, causal agent of tan spot, is one of the most important wheat leaf spot pathogens worldwide. The race stmcture of P. tritici-repentis isolates obtained from wheat (except one from noncereal grass species) in the Czech Republic was studied. The 86 monosporic isolates of P. tritici-repentis originated from different parts of the Czech Republic were grouped into five known races based on necrosis/chlorosis induction on standard differentials (Katepwa, Glenlea, 6B365, Salamouni). The isolates recovered from wheat were races 1, 2 and 4. Race 1 was the most frequent (50%), races 2 and 4 were found rarely (3% and 5% respectively). The isolate of P tritici-repentis recovered from noncereal grass species was determined as race 4 which seems to be typical for noncereal hosts. The reaction of 30 isolates (42%) was difficult to be compared to reactions of so far identified P. tritici-repentis races. They have to be tested again on additional wheat genotypes.
The aim of this studies was characteristic of the sources of primary infection for tan spot. During the period 1998 - 1991 the development of ascomata and conidimata of Pyrenophora tritici-repentis on naturally infected wheat and triticale straws was monitored. The process of pseudothecia formation was extending over the time. It started immediately after harvest and protothecia of the fungus was commonly occurred each year already in the fall. The first fruit bodies with maturated typical!y pigmented ascospores were observed in January 2001 and in February 1998 and 1999. The percentage of pseudothecia with maturate ascospores successively increased richening maximum in April when the massive liberation of spores from asci occurred. After the total ascospore release on surface of empty ascomata aroused conidiophores with conidia which mayaIso act as inoculum source. Moreover this type of sporulation was observed directly on over wintered cereals stems especially after prolonged period of rains.The present studies indicated also on infected by P. tritici-repentis wheat and triticale seeds as a source of inoculum for tan spot.
Pyrenophora tritici-repentis, anamorph Drechslera tritici-repentis, strains used in this study represented three phenotypic c1asses of this fungus: the wild type, strains unable to produce melanins - mel(-), and intermediate strains. Using an HPLC system with a reversed phase Cl18 column synthesis of anthraquinone pigments by strains belonging to all three classes could be detected. According to the 'H NMR spectra analysis the major compounds produced by P. tritici-repentis are catenarin, helminthosporin, islandicin, emodin, ω-hydroxy-islandicin, ω-hydroxy-catenarin and ω-hydroxy-helminthosporin. However, amounts of these compounds produced by the wild type were up to 350 times lower than those observed for the mel (-) forms. Some differences in profiles of these compounds, produced by different strains were also observed.
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Tan spot (Pyrenophora tritici-repentis) is one of the most important wheat diseases in Latvia. Significant spreading of this disease was observed from 1998. Field experiments were carried out at the Research and Training Farm „Peterlauki” of the Latvia University of Agriculture, during 1998-2003. Development of diseases was observed on 14 winter wheat varieties, dynamics of development was investigated on 'Donskaja polukarlikovaja' and 'Stava'. Assessments of the disease severity were carried out on the upper three leaves each week from the start of stem elongation to full ripening. Tan spot was observed at the stage of stem elongation in 2003, and only after flowering in 1999. Increase of the disease severity was slow until stage of milk ripeness, and only in late stages of wheat development sharp increase of the disease was observed. Total rate of infection (through the session of vegetation) was very slow (0,01-0,19), but during ripening rate achieved 0,5-0,6. The main reason of so unequal development of the disease seems changes in amount of infection sources. Other possible reason of rapid increase of the disease development is the relationship between leaf age and susceptibility to the disease. Further research is necessary for better understanding of tan spot life cycle.
Badano podatność odmian i rodów hodowlanych pszenicy i pszenżyta na porażenie przez Pyrenophora tritici-repentis. Doświadczenie prowadzono w warunkach prowokowanej epifitozy w polu. Ogółem przebadano 251 genotypów pszenicy oraz 328 pszenżyta. W testowanej populacji nie stwierdzono występowania form odpornych. Infekcji uległy wszystkie materiały, przy czym istotnie słabszemu porażeniu ulegało pszenżyto niż pszenica. Zarówno w przypadku pszenicy, jak i pszenżyta dominowały genotypy silnie i bardzo silnie porażane. Cechy słabego porażenia wykazywały jedynie trzy odmiany pszenicy jarej (Alkora, Ismena, Opatka), dwie pszenżyta jarego (Kargo i Leguan) i sześć rodów pszenżyta ozimego (SZD 535, SZD 539, SZD 555, SZD 599, DED 1153/96, LAD 940/96).
Badania prowadzono na polach Rolniczego Zakładu Doświadczalnego SGGW w Chylicach (woj. mazowieckie) w latach 2005–2007 na pszenicy ozimej odm. ‘Mikon’ i odm. ‘Finezja’. Warunki pogodowe w badanym okresie były bardzo zróżnicowane. Odnosi się to zarówno do rozkładu temperatury powietrza, jak i do opadów. W sezonie wegetacyjnym 2004/2005 wystąpiły znaczne spadki temperatury w lutym, po czym nastąpiła chłodna i wilgotna wiosna oraz suche i ciepłe lato. Sezon wegetacyjny 2005/2006 wyróżniała mroźna zima, trwająca ponad 4 miesiące, późna wiosna oraz dotkliwa susza w lecie. Najbardziej zbliżone do optymalnych warunki pogodowe obserwowano w sezonie wegetacyjnym 2006/2007. Zima była łagodna, maj upalny, a miesiące pozostałe do zbioru pszenicy ozimej zbliżone do normy. Tak zróżnicowane warunki pogodowe wpływały istotnie na rozwój patogenów oraz plonowanie pszenicy ozimej. W badanym okresie liście pszenicy ozimej najsilniej były porażone przez Septoria spp. i Pyrenophora tritici-repentis, kłosy przez Leptosphaeria nodorum, a podstawa źdźbła przez Tapesia yallundae i Fusarium spp. Najbardziej odbiegający od normy sezon wegetacyjny 2005/2006 zaznaczył się mniejszym porażeniem roślin przez grzyby patogeniczne, a jednocześnie długotrwała susza wpłynęła niekorzystnie na wysokość plonu ziarna. Plony pszenicy uzyskane z obiektów chronionych wyniosły odpowiednio: 2005 r. – 58,93 dt‧ha⁻¹, 2006 r. – 40,80 dt‧ha⁻¹ i 2007 r. – 65,32 dt‧ha⁻¹.
Development of tan spot epiphytosis in wheat and triticale has been investigated during three years of cropping seasons 1998-2001. The infection level of plants was scored in four stages of cereals development at the end of tillering (GS 29), shooting (GS 35-37), heading (GS 55-59) and milk maturity (GS 73-77). The monitoring performed on population of 327 wheat and 352 triticale genotypes indicated that the both cereals can be heavy infected by P. tritici-repentis, although the reaction of these hosts differed significantly. On triticale developed small, minute black spots usually without typical for wheat yellow halo. Moreover, the progress of tan spot of triticale over time was less rapid and disease intensity lower in the all plant growth stages during three years of studies.
Field experiments were carried out in the seasons 2000/2001 and 2001/2002 in Plant Protection Institute, Sośnicowice Branch to assess the influence of foliar fertilizers such as Ekolist PK 1, Ekolist Mg, Mikrosol Z and Urea on healthiness of winter wheat. Foliar fertilizers were mixed with fungicides. The fungicides were applied at full or half recommended doses. The effect of the disease on wheat leaves was evaluated three times in each vegetation season. Remaining green leaf area (GLA) of leaves was also determined. GLA of the leaves F-1 was not significantly different for each combination with different fertilization and different levels of chemical treatment. The application of foliar fertilizer only had no effect on green leaf area (GLA). The results indicate that foliar fertilization of all experimental plots improved leaf condition and therefore halted the development of wheat leaf diseases. The increases of 1000 grain mass and yield was high for each plot where a fertilizer and a full or half dose of a fungicide was applied. Foliar fertilizing with no chemical control had no proven effect on studied parameters.
A field experiment was conducted in 2007–2008 at the Brody Experimental Station of Poznań University of Life Sciences. The aim of this study was to determine leaf and stem base disease severity on winter triticale as an effect by cropping systems and fertilization. Pseudocercosporella herpotrichoides, Fusarium spp. and Gaeumannomyces graminis occurring on stem bases and roots were found in winter triticale. The incidence of stem base and root diseases was shown to increase under monoculture in comparison with crop rotation. Stem base and root diseases severity was lower after farmyard manure and NPK + Ca application than after mineral fertilization NPK. The farmyard manure + NPK fertilization increased the incidence of leaf and ear diseases of winter triticale in relation to the other fertilization treatments.
A field experiment was conducted in 2007–2009 at the Brody Experimental Station of Poznań University of Life Sciences. The aim of this study was to determine the leaf and stem base disease severity on spring wheat as the effect of different soil tillage systems (conventional and direct drilling). Fusarium spp. and Gaeumannomyces graminis occurring on stem bases and roots were the main pathogens found in spring wheat. The incidence of stem base and root was shown to increase under no-tillage in comparison with ploughing tillage system. Pyrenophora tritici-repentis was predominant on leaf. The conventional tillage increased the incidence of leaf diseases on spring wheat in relation to the ploughless tillage systems.
The winter wheat cultivar Red Chief has been identified as the wheat cultivar most resistant to Pyrenophora tritici-repentis (Ptr). This study was undertaken to determine the inheritance, chromosomal location and molecular mapping of a tan spot resistance gene in Red Chief, χ² analysis of the F₂ segregation data of the hybrids between 21 monosomic lines of the susceptible wheat cultivar Chinese Spring and the resistant cultivar Red Chief revealed that tan spot resistance in cv. Red Chief is controlled by a single recessive gene located on chromosome 3 A. Linkage analysis using SSR markers in the Red Chief/Chinese Spring F₂ population showed that the tsr4 gene is clustered in the region around Xgwm 2a, on the short arm of chromosome 3 A. This marker has also been identified as the closest marker to the tsr3 locus on chromosome 3D in synthetic wheat lines. Validation analysis of this marker for the tsr3 and tsr4 genes using 28 resistant and 6 susceptible genotypes indicated that the 120 bp allele (the tsr3 gene) specific fragment was observed in 11 resistant genotypes, including the three synthetic lines XX41, XX45 and XX110, while the 130 bp allele was amplified only in cv. Red Chief and Dashen. Xgwm2a can be used to trace the presence of the target gene in successive backcross generations and pyramiding of the tsr3 & tsr4 genes into a commonly grown and adaptable cultivar.
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