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We examined the reproductive activity and sexual behaviour of a herd of Przewalski mares Equus ferus przewalskii Poljakov, 1881 that were born in zoos and lived in a semireserve since 1992 during five periods in 1995-1997 of 4-6 weeks each Ovarian activity was detected by the analysis of faecal progestagens. In addition, behavioural detection of oestrus and continuous recording of the daily activity with a storage telemetry system were carried out and compared with the analytical data. Faecal 20a-hydroxypregnane analysis revealed ovarian activity to be 100% (April/May 1995), 25% (May/June 1996), 88% (October/November 1996), 63% (January/February 1997) and 100% (April/May 1997) of the mares sampled. Behavioural observations showed a seasonal pattern with maximal sexual interactions in April/May 1995/1997 and only few interactions in winter. Detailed activity records in individual animals revealed an oestrus related increase from 14 h/d to 15.6 h/d. Our results show a tendency of seasonality which support the view that Przewalski mares are seasonal breeders with sexual activity in spring and early summer. In May/June 1996 a dysregulation of reproductive activity associated with a persistent increase in locomotor activity occurred. We hypothesise external disturbances from a shooting yard close to the semireserve. Compared to behavioural observations, faecal progestagen analysis seem to be the most convenient method to investigate reproductive activity in free ranging Przewalski mares.
In 1998, 21 Przewalski’s horses Equus przewalskii have been introduced in the Chernobyl exclusion zone. During the years 1999–2000, studies on food preference were carried out in a special enclosure (70 ha) constructed for theses horses. A total of 52 plant species, belonging to 25 families were grazed by the horses. This comprised 43 % of all plant species recorded in this area. The most common families were Poaceae (17 species), Fabaceae (7 species) and Asteraceae (7 species). In general, Elytrigia repens, Trifolium pratense, Vicia cracca, Poa trivialis, Dactylis glomerata and Bromus inermis were the most preferred plant species. This preference has, however, varied from month to month. The most preferred plant species in May were Elytrigia repens, Corynephorus canescens, Festuca valesiaca and Chenopodium albuy; in June – Fabaceae , in July – Dactylis glomerata and Trifolium and in August – Elytrygia repens and Vicia cracca. A total of 27 anti-helminthic plant species were recorded in the study area, 18 of them were grazed by Przewalski’s horse.
A diagnostic deworming of 21 Przewalski horses, free−living in the Chernobyl exclusion zone Ukraine, and of six stabled domestic horses, has been conducted eighteen years after the Chernobyl nuclear disaster. This survey yielded 31 species (of 5 families and 3 classes, 28 species of nematodes, 1 species of cestodes, and 2 larvae of botflies). A total 29 and 19 helmith species has been recorded in the Przewalski horse and domestic horse respectively. Only six helmith species were common for the two horse species. Species from the family Strongylidae constituted the dominant helmith group. Four cyathostomine species (Cyathostomum catinatum, Cylicostephanus minutes, C. longibursatus, Cylicocyclus nassatus) formed the majority of helmith parasites both in the Przewalski and domestic horses. The presently reported study revealed that Przewalski's horses keep their typical biological features and high resistance to parasitic infections. A substantial growth of heard was observed as well as good clinical health state of horses. This can be an argument favouring the use of Przewalski horses in re-naturalization of ecological disaster areas.
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