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The molecular characteristics of partly purified sucrose synthase (NDP-glucose: D-fructose 2-α-D-glucosyltransferase, EC 2.4.1.13), extracted from the bird cherry (Prunus padus L.) leaves was elucidated. The sucrose synthase was successfully purified by using a four-step protocol including ammonium sulfate precipitation, dialysis, gel filtration on Sephadex G-150 and ion-exchange chromatography with the use of DEAE-cellulose. The analysed enzyme occurred in two isoforms (SuSyI and SuSyII). The relative molecular weight of native isoenzymes was estimated to be 200 and 180 kDa, respectively. Isoform SuSyI contained two different subunits of 57.5 and 52.8 kDa, whereas the structure of SuSyII was consisted of identical 63 kDa subunits. Experimental data indicated that the structure of both SuSy isoforms was composed of three subunits
Occurrence of the bird cherry-oat aphid (Rhopalosiphum padi L.) on bird cherry (Prunus padus L.) was studied in Eastern Poland. First fundatrix of R. padi on P. padus were observed in the first decade of April. The bird cherry-oat aphid population developed on the primary host during May. Its maximum reached the peak density until the middle of May, and in the third decade of May numerous alatae were formed and spring migration of the aphid onto the summer hosts started.
Host specialization plays a central role in physiological diversification of aphids and plant-insect co-evolution. Different morphs of the bird cherry-oat aphid (Rhopalosiphum padi L.) are specialized and locally adapted to both primary and secondary hosts. The abundance of bird cherry-oat aphid population was estimated using the technique of direct counting of aphid individuals infesting 50 randomly selected, fully expanded 1-year side shoots of bird cherry (approx. 40 cm long) grown in the Municipal Park „Aleksanria” in Siedlce (central-eastern Poland). Entomological observations were conducted during three consecutive vegetation seasons: 2001–2003. The impact of R. padi feeding on composition of free amino acids within leaves of its primary host (the bird cherry, Prunus padus L.), infested and uninfested by aphids were studied. Eight essential amino acids (Val, Thr, Phe, Ileu, Leu, Met, His and Lys) and seven non-essential amino acids (Asp, Glu, Pro, Ser, Ala, Gly and Tyr) were identified in the bird cherry-oat leaves with the use of highperformance liquid chromatography (HPLC) separation. There were quantitative differences in free amino acids content between the tested bird cherry-oat leaves (infested and uninfested by aphids). Levels of the free amino acids were decreased in leaves attacked by R. padi, and detailed analyses revealed about 20% decline in the content of studied components during maximal aphid abundance in comparison with non-infested leaves. Additionally, the feeding behavior of the bird cherry-oat aphid seasonal morphs (fundatrix, fundatrigeniae, alatae) on the primary host was investigated. Using the electrical penetration graph (EPG) we showed differences in feeding behavior of bird cherry-oat aphid morphs on primary host. The total probing time was greatest for fundatrigeniae among all studied morphs. These individuals spent more time during phloem sap ingestion then others. Adults of alatae were not active in the phloem sap ingestion on the bird cherry. Among the tested morphs xylem sap ingestion activity was greatest for alatae. It is hypothesized that rejection of primary host and subsequent the bird cherry-oat aphid migration onto secondary hosts may be stimulated by the decline in levels of free amino acids within primary host leaves caused by long-term R. padi feeding. Comparative analyses of the aphid probing behavior additionally proved that P. padus leaves possessed high nutritive value of food for non-winged morphs (early stages of aphid infestation), while not sufficient for winged alatae (terminal phase of infestation).
The activity of beta-cyanoalanine synthase and rhodanese within the tissues of the three generations of the bird cherry-oat aphid that are specialized to feed on the cyanogenic spring foliage of bird cherry was assessed. The order of beta-cyanoalanine synthase activity in the three aphid generations was: generation 1> generation 2> generation 3; whereas that of rhodanese was: generation 3> generation 1>generation 2. The possible role of the bird cherry – oat aphid enzymes in metabolizing dietary cyanide is discussed.
Changes in content of cyanogenic glycosides and in cyanogenesis potential within the bird cherry leaves during occurrence of the bird cherry-oat aphid, Rhopalosiphum padi L. and while its spring host-plants alternation have been studied. The highest content of the cyanogenic glycosides and the highest cyanogenesis potential was found in the youngest leaves of the primary host during occurrence of the first fundatrices. When the aphid population started to build up, a decrease in content of the cyanogenic glycoside and in the cyanogenesis potential was observed. Finally, when the winged migrants began to fly off from the primary host onto cereals, pretty low amount of the plant xenobiotics was recorded within the bird cherry leaves. Possible role of the cyanogenesis in host alternation of the bird cherry-oat aphid is discussed.
Determination the quantitative and qualitative amino acid composition of albumins and globulins isolated from two cultivars of winter triticale was done using of ion-exchange chromatography. The fifteen protein amino acids in the composition of albumins and globulins were identified and determined. The present study proved that the smaller amounts protein amino acids in the fractions albumins and globulins isolated from the Malno cultivar plays an important role in the constitutive resistance to grain aphid.
The host-plants of bird cherry-oat aphid were compared as to the content and composition of phenolic compounds. The gallic, chlorogenic, caffeic, siringic, o- and p‑coumaric, tannic, ferulic, sinapinic, salicylic, p‑hydroxybenzoic acids and /+/‑catechin were detected in the all studied host-plants of the bird cherry-oat aphid. Coumarin and vanilic acid only occurred within orchard grass and winter triticale tissues. Predominant compounds within P. padus leaves were /+/‑catechin, chlorogenic acid, p-hydroxybenzoic acid and tannic acid. Vanilic and salicilic acids were the major compounds of the orchard grass, whereas leaves of winter triticale contained mostly salicilic, chlorogenic and vanilic acids as well as coumarin.
The bird cherry-oat aphid (Rhopalosiphum padi L.) development on primary host (Prunus padus L.) was studied in Poznan in 2007–2008. First fundatrices of R. padi were observed already on the bird cherry in March in both years. The largest condensation of population was observed in May. At the second decade of May numerous winged morphs were formed and spring migration into the secondary hosts started. Re-emigrants in September were observed and the oviparae and males in second decade of October gave birth. In spring observations fertilized eggs (from which aphids hatched) made up 89% and 81% respectively. The meeting of factors inducing the formation of individual morphs of R. padi and the deadlines of the migration on host-plants has a special meaning in the aspect of transfer of viruses by the anholocyclic forms of this species.
This article studies the effect of light and high temperature on various parameters describing the growth and development of bird cherry ermine moth (Yponomeuta evonymellus L.) – the main pest of bird cherry (Prunus padus L.). We found that light significantly influences the moth masses and dynamics of their development. Moths developed from larvae feeding on leaves growing in full light conditions have a higher mass and shorter time of adult eclosion from pupa in comparison with larvae feeding on leaves growing in shade. However, extremely high summer temperature significantly disturbs pupation process in Y. evonymellus population. Our laboratory experiments indicate that a critical temperature is 36°C. Above this threshold the percentage of pupation drastically decreases (from ca. 70% to 35%). The conditions of intensive sun are better for some parameters of growth and development of bird cherry ermine moth. On the other hand, extreme summer temperatures, related with global warming, may restrict the occurrence of Y. evonymellus gradation.
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Despite senescence-induced chlorophyll depletion in plants has been widely studied, the enzymatic background of this physiologically regulated process still remains highly unclear. The purpose of this study was to determine selected biochemical properties of partially purified fractions of chlorophyllase (Chlase, chlorophyll chlorophyllido-hydrolase, EC 3.1.1.14) from leaves of three Prunus species: bird cherry (Prunus padus L.), European plum (Prunus domestica L.), and sour cherry (Prunus cerasus L.). Secondarily, this report was aimed at comparing seasonal dynamics of Chlase activity and chlorophyll a (Chl a) content within investigated plant systems. Molecular weight of native Chlase F1 has been estimated at 90 kDa (bird cherry) and approximately 100 kDa (European plum and sour cherry), whereas molecular mass of Chlase F2 varied from 35 kDa (European plum) to 60 kDa (sour cherry). Furthermore, enzyme fractions possessed similar optimal pH values ranging from 7.6 to 8.0. It was found that among a broad panel of tested metal ions, Hg+2, Fe+2, and Cu+2 cations showed the most pronounced inhibitory effect on the activity of Chlase. In contrast, the presence of Mg+2 ions influenced a subtle stimulation of the enzymatic activity. Importantly, although Chlase activity was negatively correlated with the amount of Chl a in leaves of examined Prunus species, detailed comparative analyses revealed an incidental decrement of enzymatic activity in early or moderately senescing leaves. It provides evidence that foliar Chlase is not the only enzyme involved in autumnal chlorophyll breakdown and further in-depth studies elucidating this catabolic process are required.
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