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1

Selection of tomato plants resistant to a local Polish isolate of tomato spotted wilt virus [TSWV]

100%
Czech A. S., Szklarczyk M., Gajewski Z., Zukowska E., Michalik B., Kobylko T., Strzalka K.
Journal of Applied Genetics
|
2003
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tom 44
|
nr 4
2

Full length genome sequence of Polish isolate of Beet soil-borne virus confirms low level of genetic diversity

84%
Borodynko N., Hasiow-Jaroszewska B., Rymelska N., Pospieszny H.
Acta Biochimica Polonica
|
2009
|
tom 56
|
nr 4
729-731
 The complete nucleotide sequence of a Polish isolate of Beet soil-borne virus was determined for the first time. The genome organization was identical with those previously established for isolates from Germany and China. A comparison of the Polish isolate with others deposited in GenBank reveled high level of nucleotide identity, about 98-100%, throughout the genome analyzed. The ratio between non-synonymous and synonymous substitutions was rather low suggesting a negative selective pressure. The non-synonymous mutations were particulary frequent in triple gene block.
3

Serological variation among Polish isolates of Yersinia ruckeri

67%
Pekala A., Kozinska A., Antychowicz J.
Bulletin of the Veterinary Institute in Puławy
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2010
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tom 54
|
nr 3
One hundred and twenty-six isolates of Yersinia ruckeri originating from different species of fish were collected: 122 from rainbow trout (Oncorhynchus mykiss; Walbaum), three from pike (Esox lucius L.), and one from carp (Cyprinus carpio L.). The O- serotyping of the isolates were carried out for the first time in Poland by microplate agglutination assays according to the Davies procedure. Three O-serotypes were determined: O1, O5, and O7. Serotypes O2 and O6 have not been recognised. Almost all isolates were represented by serotype O1, which originated only from rainbow trout showing classical clinical signs of enteric redmouth disease. The strains representing serotype O5 were only collected from pike and serotype O7 from carp and rainbow trout showing no clinical signs of the diseases.
4

Evaluation of Polish and tropical isolates of the nematophagous fungus Verticillium chlamydosporium Goddard for the control of the root-knot nematode [Meloidogyne incognita Kofoid and White] on tomato plants

67%
Sosnowska D., Kerry B. R.
Bulletin of the Polish Academy of Sciences. Biological Sciences
|
2002
|
tom 50
|
nr 1
5
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Genetic analysis of Polish isolate of swine vesicular disease virus [SVDV POL-73] by RT-PCR and nucleotide sequencing of the VP1-2A coding region

67%
Niedbalski W.
Bulletin of the Veterinary Institute in Puławy
|
1998
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tom 42
|
nr 1
A nucleotide sequence analysis by RT-PCR and cycle sequencing of the Polish isolate of swine vesicular disease virus (SVDV) was performed. The RT-PCR products pattern and VP1/2A coding region sequence were compared to the other available European isolates of SVDV and the human Coxackie virus isolate CAV-16. The results of the RT-PCR show that it is possible to detect different isolates of the SVDV however Coxackie virus-specific PCR products were obtained only by the use of one from three selected primer pairs. A sensitivity of the used RT-PCR was equivalent to that of virus isolation in cell culture and even higher than the ELISA method. The considerable nucleotide similarity between the Polish isolate UKG27/72 and the Japan 76 SVDV isolates (99% and 98%, respectively) as well as the CAV-16 (96%) was found. SVDV Poland 73 was genetically less related (about 88%) to the Dutch and Italian isolates from the recent outbreaks of disease in 1992. Presented results confirmed the quasispecies character of these picornaviruses.
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