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The antifungal properties of selected essential oils, i.e. lavender, mint, orange, spruce and thyme oils, were checked in vitro to fungi isolated from blue spruce (Picea pungens Engelm.) and its cultivar ‘Glauca’. The degree and extent of antifungal activity for essential oils listed above were tested on the following fungi: Acremonium tubakii, Anthostomella conorum, Arthrinium state of Apiospora montagnei, Aureobasidium pullulans, Botryodiplodia rubi, Fusarium camptoceras, F. moniliforme var. lactis, Penicillium canescens, Phoma pomorum, Rhizosphaera kalkhoffii, Ulocladium consortiale and Zythiostroma pinastri. Among essential oils tested the thyme oil showed the highest antifungal activity to all fungi under examination even at the lowest concentration used. The mint, lavender and spruce oils demonstrated a medium activity. The lowest activity was found for orange oil.
Somatic embryogenesis was studied in four spruce species (Picea abies, P. omorika, P. pungens 'Glauca' and P. brewenana) to determine if this method can be used for in vitro propagation of coniferous trees. The highest frequency of initiation of embryogenic tissue was obtained when mature zygotic embryos were used as explants. It ranged then from 10.8% (P. brewenana) to 23.75% (P. omorika and P. pungens 'Glauca'). The frequency of embryogenic tissue initiation was strongly affected by medium composition, i.e. addition of appropriate auxins (2,4-D, NAA, Picloram) and sucrose concentration (10-20 g-1"1). A lower frequency was obtained in Picea omorika (10%) when megagametophytes (endosperms with immature zygotic embryos) were used as explants. No emryogenic tissue was produced from hypocotyls, cotyledons and needles. A satisfactory frequency was achieved with the use of somatic embryos of Picea abies (30%). The proliferation of embryogenic cell lines of spruces was affected by medium type. The experiments resulted in production of somatic plantlets of P. abies and P. omorika. This enables the application of this method of spruce micropropagation for genetic and breeding research or for nursery production.
The antifungal activity of two fungicides, i.e. Rovral Flo 255 SC and Sportak Alpha 380 EC was determined by in vitro tests to 12 fungal species isolated from blue spruce (Picea pungens) and its cultivar Glauca. It was found that Sportak Alpha 380 EC has the highest antifungal activity and the widest range of application to all fungi under examination. Rovral Flo 255 SC has shown significantly poorer activity.
The aim of the study was to determine the causes of dying down of sprout and needles of Colorado blue spruce (Picea pungens Engelm.) and its cultivar ‘Glauca’ in the park collection of the Botanic Garden of Jagiellonian University in Cracow. For several years, the disease symptoms described in the article have led to the loss of decorative values of these trees. The study was conducted in the years 2002 and 2003 by means of the control of plant healthfulness, phytopathological diagnostics, isolation of microorganisms from tissues exhibiting diseases symptoms, as well as the culture and identification of the isolated fungi. Both, the control of tree healthfulness, and specialized laboratory techniques proved that the symptoms diagnosed in plants had an infectious character, and that the fungi might be the main causing agents. The analysis of the fungal populations isolated from the affected tissues indicates the combined occurrence of fungi in tissues of the studied plants and the domination of the following species: Alternaria consortiale, Aureobasidium pullulans, Penicillium canescens, Phoma pomorum, Ulocladium consortiale, Zythiostroma pinastri.
These studies were undertaken to identify the reasons for decay of spruce and juniper trees growing near the Metalplast plant in Oborniki. The material from the trees growing in this region was analyzed for the presence of free and complex-bound fluorine (FA) and total content of fluorine (FB), as well as some metals known to hae harmful effects on plants. The results were compared with analogous data obtained for plants growing in the area free from this kind of pollution, in Kórnik. The comparison has confirmed the hypothesis claiming that the main reason for decay of trees of the above species growing in the neighbourhood of the Metalplast plant in Oborniki is pollution by fluorine compounds.
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