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A collection of 14 species of Patagonian plants was evaluated for tocopherol content and composition, plastochromanol, plastoquinone content and polyprenol composition. Total tocopherols varied from 35.77 µg/g in Mutisia decurrens to 798 µg/g in Philesia magellanica. In most cases tocopherol composition was dominated by α-tocopherol, which accounted for more than 90% of total tocopherols. Of all the investigated species only Misodendrum punctulatum showed higher content of γ- than α-tocopherol, which is unusual for mature leaves. Plastochromanol, a homologue of γ-tocotrienol, was found in leaves of 10 of the 14 examined species, and was highest in Araucaria araucana leaves (132 µg/g). Total content of PQ-9 (oxidized and reduced) was highest in Fuchsia magellanica (774.3 µg/g), Philesia magellanica (791 µg/g), Misodendrum linearifolium (569 µg/g) and Amomyrtus luma (518.5 µg/g). Analysis of polyprenol content in the leaves of investigated plant species revealed detectable amounts (> 10 µg/g d.w.) of polyprenyl esters in six of them. Accumulation of free polyprenols was detected only in Chusquea quila leaves. Selected organs of Philesia magellanica and Fuchsia magellanica were further quantitatively analyzed for tocochromanol and polyprenol content. With the methods applied, different patterns of the analyzed compounds were identified in all the samples studied. Our results reveal some trends that may be of taxonomic interest. Some of these species can serve as a rich source of such bioactive compounds as tocochromanols or polyprenols.
Estimates of locomotory speeds of small to large-sized Patagonian dinosaurs are presented for the first time. These estimates are inferred from trackways found on fine to coarse-grained brown sandstones located in the lower section of the Candeleros Member of the Río Limay Formation (Albian-Cenomanian), Neuquén Province, Argentina. The method used is based on the measurement of the stride length (distance between two successive prints of the same foot) and of the length of the hindfoot print, which in turn, allows us to estimate the height at the hip joint and, therefore, the approximate size of the animal. The hypothesis of dynamic similarity implies that the movements of geometrically similar animals, although of different sizes, are dynamically similar only when they move with the same Froude number. The dynamically similar movements (i.e., those with equal Froude number) require equal values of relative stride length (ratio between the stride length and the hip joint height). The relationship between the relative stride length and the Froude number allows us to estimate the speeds of dinosaurs. The dinosaurian ichnofauna studied reveals low speeds that range from 0.5 to 2.6 m s⁻¹. Our analyses show that the sauropods responsible for these trackways were either walking very slowly in a bipedal stance or alternatively they were progressing quadrupedally on a slippery surface.
We used long-term datasets (1984–1992) to contrast precipitation-use efficiency estimates between various disturbance kinds at a functional group and/or a species scale. Effects of varying amounts of precipitation and plant cover on PUE were also examined. Field studies were conducted at northeastern, arid Patagonia, Argentina (40°39′49″S, 62°53′6.4″W). Within each management kind, biomass was sampled in 0.5 × 0.5m permanent plots (n = 30) over 9 years after defoliation at 5 cm stubble at the end of each growing season, and it was separated into species. Biomass sampling allowed determination of annual net primary production. Thereafter, species were grouped into each of three functional groups. Precipitation-use efficiency (PUE) was calculated as the total dry matter produced per unit surface area on any given year divided by the total rainfall in that year. Plant cover on 20 out of those 30 plots was determined to study the relationship between plant cover and PUE. The contribution of cool-season perennial grasses to total PUE was higher (P <0.05) than that found for the other two functional groups in all management kinds and years. PUE was similar (P> 0.05) in wet than dry years, and it was greater (P <0.05) or similar (P> 0.05), but not lower, on the more than less competitive perennial grass species in all management kinds. The relationship between plant cover and PUE was positive, linear (P <0.0000) and management-kind dependent.
A new, relatively diverse gastropod fauna is reported from the Chubut province of west−central Patagonia. The gastropod association at the “El Córdoba” fossiliferous locality (Lower Toarcian of Osta Arena Formation) consists of three new species: the eucyclid Amberleya? espinosa sp. nov. and two procerithiids Cryptaulax damboreneae sp. nov. and Cryptaulax nulloi sp. nov. Other members of the association are the ataphrid Striatoconulus sp., discohelicid Colpomphalus? sp., and an undetermined zygopleurid. Knowledge on Early Jurassic gastropods from South America and other southern continents is reviewed to show that the taxonomic composition of the El Cordoba association strongly resembles other gastropod associations of this age (even those from Europe), suggesting a wide distribution of cosmopolitan genera.
Bezzia galesa Spinelli, a new Patagonian species, is described in all stages and illustrated by using binocular, phase-contrast and scanning electron microscopy. Immatures were collected associated to submerged filamentous algae in an unnamed pond in western Chubut province, Argentina, and reared to adults in the laboratory. Adults and immatures of this new species are compared with the most similar species Bezzia ventanerisis Spinelli, Bezzia roldani Spinelli et Wirth and Bezzia blantoni Spinelli et Wirth. Details on the rearing process and feeding behavior in laboratory are given.
Monthly samples totalling 202 European hares Lepus europaeus Pallas, 1778 were collected in southern Argentina from August 1993 to March 1994. The sex ratio of the whole sample was 1:1.2 in favour of females. Pregnant females were present from August to February and lactating females were present from September to March. The percentage of pregnant females was 59% for the whole breeding season. The mean ovulation rate for female hares was 2.78; the number of ova ovulated was not sig­nificantly correlated with body weight. The mean number of implantation sites per reproducing female was 2.46. The number of embryos in a litter varied from 1 to 4, the average being 2.12. The prenatal mortality was 56.5% of all ova ovulated and of these 34.7% were lost before implantation and 21.8% after implantation.
Diet of Patagonian hog-nosed skunk Conepatus humboldtii Gray, 1837 was studied in southeastern Argentinean Patagonia. Faeces from skunks were collected and analysed on a yearly basis. A wide variety of prey ranging from invertebrates (81.6% of occurrences), mammals, reptiles and plant material occurred in the faeces. Though Patagonian hog-nosed skunk was reported as primarily insectivorous regarding its feeding anatomy and behaviour, vertebrate prey predominated over invertebrates when results were expressed as consumed biomass. The consumption of invertebrate prey increased during summer, probably due to an increase in its abundance. The increase in the consumption of rodents and carrion by skunks during autumn-winter could be due to a shift from open grassy areas to other habitats to find alternative prey.
The southern vizcacha (Lagidium viscacia) and the exotic European hare (Lepus europaeus) are two medium-sized herbivores that inhabit rocky outcrops in Patagonian steppe. These species overlap in diet and spatial use at medium distances from rocky outcrops in summer. We evaluated the spatial use through feces distribution in winter and determined seasonal foraging intensity in relation to the distances from rocky outcrops in order to elucidate how these herbivores use food and spatial resources in food scarcity periods. The vizcacha utilized the habitat close to rocky outcrops (<40 m) independent of season, while the hare exploited the space more widely, especially distances >40 m. However, in winter, at medium distances from rocky outcrops, there was partial spatial overlap because hares' activities were closer to rocky outcrops. Foraging intensity increased significantly in areas used by the vizcacha closer to rocky outcrops when food availability decreased, and the grasses Stipa speciosa, Poa sp., and Festuca pallescens were strongly foraged. In contrast, foraging intensity showed no changes in further distances to rocky outcrops and more use by the hare. The spatial and feeding behavior of the vizcacha, restricted to vicinity of rocky outcrops, showed high vulnerability to food availability changes. In resource scarcity situations, the spatial opportunistic behavior of the hare and the overlap in diet with the vizcacha constitutes a threat to this native herbivore. It is necessary to monitor populations of hare, since high densities could lead to food competition, impacting the small colonies of the southern vizcacha.
The major point for Lacar Demosite (LDS) is the sustainable management of a representative basin of Andean Patagonian region where the main problem is the impact of land transformation on water quality and environmental services. The aim of the LDS is the reduction of soil erosion, the mitigation of floods in urban areas and the improvement of ecosystem services, based on Ecohydrological ideas and principles, and using phytotechnologies. Advances are presented for LDS showing that regulation of surface hydrology (water runoff; nutrients and sediments loads) at the watershed scale, through land use, vegetation cover and phytotechnologies, improve the aquatic ecological processes and the ecosystems services (water quality, landscape aesthetic values).
Two fossil tadpoles collected in the Deseadan levels (Oligocene) at the Scarritt Pocket locality of central Patagonia are studied herein. These specimens, which show different degrees of skeletal development, have been assigned to the neobatrachian Calyptocephalella canqueli based on the morphology of the frontoparietals and the presence of adult specimens of this fossil species at the same locality. The concurrent analysis of three developmental stages (Gosner Stages 35/36 and 38/39, and adult) has provided significant data about the ontogeny of this species, including the change of the pattern of exostosis of the frontoparietals, from a pitted to a tuberculated pattern, and the corroboration of the inclusion of two neural arches in the formation of the urostyle. This evidence will shed light on developmental mechanisms that might be involved in the evolution of the genus Callyptocephalella.
Neuquensaurus, from the Late Cretaceous of Argentina and one of the first dinosaurs described from Patagonia, is one of the most derived sauropod dinosaurs, and its proportions and size place it among the smallest sauropods ever known. In this context, Neuquensaurus is central to understanding late stages of sauropod evolution. This contribution offers a full description of the appendicular skeleton of Neuquensaurus. The anatomical analysis reveals that the appendicular skeleton of Neuquensaurus exhibits unique characteristics only shared with closely related saltasaurine titanosaurs; for example, the laterally directed preacetabular lobe of the ilium, the prominent fibular lateral tuberosity, and the presence of an intermuscular line on the femoral shaft, which is proposed here as a synapomorphy of Saltasaurinae. Neuquensaurus also displays many reversals to primitive character states, such as the presence of a prominent olecranon process of the ulna, a trochanteric shelf, a lesser trochanter and an ischial tuberosity. Additional characters that allow its evaluation in a phylogenetic context are here provided. Among them are the extremely deflected femoral shaft, the elliptical femoral cross−section, and the anterolaterally oriented cnemial crest.
A new species of Austrelmis, A. uaik sp. nov., from Patagonia, is described from adults of both sexes. Mature larvae of this species are also described and illustrated in detail. Adults of A. uaik are compared with those of A. patagonica, the only other species known from Patagonia and with other described argentine Austrelmis species. Larvae of A. uaik are compared to other previously described larvae from Argentina. A key to the known species of Austrelmis known from Argentina is included.
The acuarioid nematode Echinuria skrjabiniensis Efimov in Skryabin, Sobolev et Ivashkin, 1965 was found in Calidris bairdii and C. fuscicollis (Aves, Scolopacidae) examined from several locations from Patagonia, Argentina. These constitute new host records as well as the first record of this parasite species in South America. Using both light and scanning electron microscopies, new morphological details are provided, including the description of the left spicule and the number and the arrangement of male caudal papillae. The taxonomic history of the species is summarized, corroborating its correct spelling and valid name.
The Unenlagiinae is a clade of Gondwanan dromaeosaurid theropods mainly known from incomplete skeletal material. The group includes two recently discovered theropods, Buitreraptor and Austroraptor, from which cranial remains are available with in situ maxillary and dentary teeth, thus allowing the study of tooth morphology. Among the derived traits that diagnose the dentition of unenlagiines are: (i) high tooth count, (ii) small size of individual teeth when compared with skull height, (iii) absence of denticles and carinae, and (iv) presence of longitudinal grooves on the tooth crown. This suite of dental characteristics, shared between Buitreraptor and Austroraptor, can be considered as diagnostic of the Unenlagiinae or, at least, a more exclusive clade within the group. The teeth of Buitreraptor exhibit a remarkable labiolingual compression, whereas Austroraptor possesses more conical teeth, probably respective autapomorphic features. On one hand, these dental morphologies differ from those observed in most Laurasian dromaeosaurids and, for instance, could be considered as further proof of the purported vicariant evolution of the lineage on the southern continents. On the other hand, the morphological similarities (e.g., absence of denticles) between the teeth of unenlagiines and other theropod lineages, including Mesozoic birds and ornithomimosaurs, can be considered as the result of parallel trends related to dental reduction.
Knowledge of the latest Late Cretaceous mammalian fauna in the South America was, until now, mostly based on dentally known taxa recovered at Los Alamitos (Río Negro, Argentina). Here we describe new mammalian remains collected in outcrops of the La Colonia Formation (Campanian–Maastrichtian) exposed in Chubut Province, Argentina, warranting the recognition of a new mesungulatid: Coloniatherium cilinskii gen. et sp. nov. The mammalian high−level taxonomic compositions of the localities in the La Colonia Formation and at Los Alamitos are roughly similar (Reigitheriidae, Mesungulatidae, and Ferugliotheridae are represented in both localities), but gondwanatheriids and the more plesiomorphic dryolestoids from Los Alamitos are missing from La Colonia. The most abundant mammalian remains collected at La Colonia correspond to large−sized mesungulatids. Coloniatherium cilinskii is recognized by the dentition and lower jaw, and we assign five isolated petrosal bones, focusing our study primarily on the analysis of the ear regions. The morphology of the petrosals suggests a phylogenetic position similar to Vincelestes, but sharing some derived features, possibly convergent, with therians. Attribution of the petrosals to the mesungulatid Coloniatherium cilinskii is supported by overall morphology, size, and relative abundance among the mammalian remains from La Colonia.
The southern vizcacha (Lagidium viscacia) is a rock specialist that inhabits small colonies in isolated rocky outcrops of northwestern Patagonia. This study analyzes its diet selection in relation to food availability, establishes the degree of dietary specialization, and discusses the potential competition with exotic herbivores. Diet composition and food availability were determined in summer and winter in eight rocky outcrops by microhistological analysis of fecal pellets, and food availability was estimated by the Braun Blanquet cover abundance scale. Vegetation cover differences were detected by using a random analysis of variance (ANOVA) factorial block design, and dietary preferences were determined by the confidence interval of Bonferroni. The southern vizcacha showed a specialized feeding behavior despite the consumption of a wide variety of items. Their diet was concentrated on a few types of food, mainly grasses, and the trophic niche was narrow and without seasonal variations. In winter, when food was scarce and of lower quality than summer, diet was dominated by Stipa speciosa, suggesting a selection according to the selective quality hypothesis. Our results (narrow trophic niche, restricted activity near rocky outcrops, and a diet with high proportions of low-quality grasses) showed that the vizcacha is an obligatory dietary specialist, and these characteristics made it highly vulnerable to changes in food availability. In this scenario, overgrazing caused by alien species with similar diets, as the European hare and livestock, could negatively affect their colonies.
A new gastropod fauna is reported from Chubut province of west−central Patagonia. Members of Trochoidea, Pseudo− melanoidea, Campaniloidea, and Nerinoidea are recorded from the Early Jurassic (Late Pliensbachian–Early Toarcian) of Mulanguińeu Formation. The gastropod fauna consists of two new species: the pseudomelaniid Pseudomelania feruglioisp. nov. and the protorculid Anulifera chubutensissp. nov. Other members of the association are Pseudomelania sp.; the ampulloispirids Globularia cf. catanlilensis, Globularia sp., and Naricopsina? sp.; the nerineids Nerinea? sp. 1 and Nerinea? sp. 2; the trochids Lithotrochus humblodtii, Lithotrochus cf.rothi, and two indeterminable trochids species. An analysis of diversity was made considering all gastropod fauna recovered so far from five fossiliferous localities sampled in west−central Patagonia. The preliminary results of this study suggests that the Jurassic marine sequences of west central Chubut province are dominated by gastropods of Eucyclidae, Pseudomelaniidae, Procerithiidae, and Ampullinidae groups. However, the rarefaction curves of particular marine gastropod faunas in the Jurassic of Patagonia are still far from saturation requiring further collecting effort.
Two types of metacercariae with 37 and 43 collar spines were obtained from Diplodon chilensis (Unionacea, Pelecypoda). They were fed to chicken and mice and mature adults with eggs were only obtained from the latter. These are described as a new species, Echinoparyphium megacirrus sp. n.
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