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Some organs of the reproductive system of the protogynous monogenean skin parasite Macrogyrodactylus congolensis (Prudhoe, 1957) Yamaguti, 1963 have been studied using transmission electron microscopy. The vesicula seminalis is enclosed by a prominent layer of circular muscle fibres and has inner syncytial protrusions. The penis bulb is a highly muscular organ with prominent radial and circular muscle fibres, a gutter-shaped large spine and 16 small spines. Two syncytial male accessory glands, and a single reservoir for male accessory secretion were identified. The secretory bodies in the male accessory glands and male accessory reservoir have a unique structure. A large oocyte is situated in a chamber, previously referred to as the “ootype” or “egg-cell-forming region” (ECFR), which also contains one or two small undifferentiated cells and vacuolated tissue. Mature spermatozoa were abundant in the receptaculum seminis and dispersed in the vacuolated tissue in the ECFR and appeared to be attached to the membrane of the large oocyte. Mature spermatozoa were also seen in the parenchymal tissue near the chamber containing embryos and even in the tissues of the embryo.
Thirty-four identified in addition to five unidentified species appertaining to ten genera of zoosporic fungi were identified and isolated from eighty four polluted water samples, which were randomly collected from different polluted sites of the water drainages along the Niles Delta in Lower Egypt. Baiting sesame seeds culture technique was employed at 20±2°C for the recovery of zoosporic fungi. The genera; Pythium and Saprolegnia (8 and 7 zoosporic fungal species, respectively) showed the broadest spectra of species diversity whereas Aqualinderella was only represented by one species (A. fermentans). Saprolegnia delica and Dictyuchus carpophorus(the greatest fungal populations) were the most dominant isolated zoosporic fungal species where they were highly occurred especially at the hyperpolluted waters with the heavy metals. These two species could be considered as indicators for the response of the structure and function of microbial communities for water pollution. Several zoosporic fungal species were rarely encountered. Both Aqualinderella fermentansand Pythium rostratumwere recovered in moderate frequency of occurrence. Water samples which had high concentrations in heavy metals were the poorest in the species diversity of zoosporic fungi. Despite that, fungal species belonging to the family Saprolegniaceaeflourished in hyper polluted water samples whilst those belonging to the family Pythiaceae predominated in more diluted water samples. Also, the prevalent species; S. delica andD. carpophoruswere not affected by heavy metals concentrations being as indicators for water pollution with the heavy metals. pH values of the polluted water samples had no influence on the occurrence of zoosporic fungi. Water samples characterized by high organic matter content and low total soluble salts were the richest in zoosporic fungal species.
The river Nile is a dominating physical feature of the north- eastern part of the African continent, covering areas exhibiting varieties and contrasts in topography, climate vegetation and people. So, in this study, we estimated the contamination of the Nile sediment with some major and trace heavy metals in core samples which can cause some undesirable health effects. Concentrations of all elements shows that the maximum enrichment is found in the top subsamples, while the most elements shows low contents in the lower layer reflecting the impact of human activity on the sediment contents. Different normalizing methods were used, i.e. geoaccumulation (Igeo) and enrichment factor (EF), for the influence of the natural variability in sediment mineralogy and to assess whether the concentrations observed in surface sediment represent background or contaminated levels. The study has also shown the importance of different sediment characteristics in spatial distribution of heavy metals in the sediments of river Nile. To protect the ecological system of river Nile watershed, industrial mining and agricultural activities along river stream should to be strictly regulated.
Generally, the number of genera and species of microflora were found to have decreased in the River Nile water treated with different doses (0.1-10 g dm-3) of four detergents (Biocleana, Lang, Omo and Tide). Algae are more sensitive to detergents than bacteria and fungi but some species were increased either slightly or markedly. No stimulatory effect was observed on bacteria by any of the detergents. The effect of detergents on fungi was different. The most tolerant fungal species was Penicillium chrysogenum Thom and may be regarded as a detergent- (pollution-) tolerant fungus.
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