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An improvement of cucumber cotyledon greening bioassay for cytokinins

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Yu X., Li G., Xu D., Dong X., Qi X., Deng Y.

Acta Physiologiae Plantarum

2006

tom 28

nr 1

The cucumber cotyledon greening bioassay for cytokinins was modified by using 95 % acetone -ethanol in stead of 80 % acetone as ex traction solvent. The cotyledons were ex tracted directly with a 2:1 (v/v) ac e tone-ethanol solution in dark for 24 hours, omitting the homogenization and centrifugation operations of the previous bioassay. The modified bioassay is more convenient and especially use ful in screening cytokinin-active substance from a large number of samples.

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1 Acta Physiologiae Plantarum

1 Deng Y.

1 Dong X.

1 Li G.

1 Qi X.

1 Xu D.

1 Yu X.

1 2006

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An improvement of cucumber cotyledon greening bioassay for cytokinins