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1

Wystepowanie Mycobacterium paratuberculosis w mleku surowym

100%
Szteyn J., Wiszniewska-Laszczych A., Ruszczynska A.
Medycyna Weterynaryjna
|
2006
|
tom 62
|
nr 10
1186-1187
The aim of the study was to determine the frequency of Mycobacterium paratuberculosis occurrence in raw milk samples from north-eastern Poland. Samples of udder milk were collected from herds in which seropositive reactions on paratuberculosis had been confirmed in samples of bulk tank milk from production farms. Standardization and decontamination of milk samples from fast growing microflora was carried out prior to research which was performed by using the culture method. PCR technique was used to distinguish MAP from other mycobacterium, thus enabling the IS-900 fragment to be detected. The presence of MAP was confirmed in 2.4% of udder milk samples from north-eastern Poland, but none was confirmed in bulk tank milk samples from this region.
2

PCR w wykrywaniu Mycobacterium paratuberculosis w mleku surowym

100%
Szteyn J., Wiszniewska-Laszczych A., Ruszczynska A.
Medycyna Weterynaryjna
|
2007
|
tom 63
|
nr 09
1106-1107
The aim of the study was to detect Mycobacterium paratuberculosis DNA in raw milk samples. DNA from 103 udder milk samples was isolated using the eQIAamp DNA Mini Kit (Qiagen). IS-900 - a part of genome characteristic for MAP - was detected in 21 samples.
3

Development of methods for the isolation of acid-fast mycobacteria from milk

86%
Szteyn J., Wiszniewska A., Fus M. M.
Polish Journal of Natural Sciences. Supplement
|
2004
|
tom 02
157-161
4

Detection methods of Mycobacterium paratuberculosis

86%
Wiszniewska A., Szteyn J.
Polish Journal of Veterinary Sciences
|
2002
|
tom 05
|
nr 3
5

Przydatnosc roznych podlozy i preparatow czynnika wzrostowego mycobactin do hodowli Mycobacterium paratuberculosis

72%
Zorawski C., Lipiec M.
Medycyna Weterynaryjna
|
1993
|
tom 49
|
nr 08
377-379
The growth rate and the number of positive cultures of M. paratuberculosis on Nemoto’s, serum agar and Herrold’s media with the addition of mycobactins have been compared. The mycobactins were prepared from M. phlei, M. intracellulare, M. avium and M. tuberculosis. The suspensions of M. paratuberculosis culture or tissues, intestines and lymph nodes, collected from cattle affected with John’s disease were used for inoculation. The best results were obtained on Nemoto’s medium with the addition of a dried mass of M. phlei or M. paratuberculosis, strain TEPS.
6

Laseczki paratuberkulozy w mleku

72%
Wiszniewska A., Szteyn J.
Nowa Weterynaria
|
2000
|
tom 05
|
nr 1
30-31
7

Badanie ryzyka wystapienia paleczek z rodzaju Mycobacterium w stadach bydla mlecznego

72%
Szteyn J., Wiszniewska A.
Przegląd Mleczarski
|
2002
|
nr 05
201-204
8

Effectiveness of Mycobacterium paratuberculosis isolation from raw milk by means of direct isolation of DNA and classic culture

58%
Szteyn J., Wiszniewska-Laszczych A., Ruszczynska A.
Polish Journal of Veterinary Sciences
|
2008
|
tom 11
|
nr 1
25-28
Detection of Mycobacterium paratuberculosis (MAP) in tissues of patients suffering from Crohn's disease has given rise to speculation that this mycobacterium may play some role in the development of this disease in humans. Food products, especially milk obtained from animals infected with paratuberculosis, may be a potential vector of MAP to humans, yet the detection of this pathogen poses a number of difficulties. This study was aimed at comparing the effectiveness of MAP isolation from milk samples. Mycobacteria were detected by means of two methods: direct isolation of DNA using a QIAamp DNA Mini Kit by Qiagen, and a culture method with the use of HEYM culture medium. Analyses were carried out on 87 samples of udder cow milk originating from a herd that exhibited seropositive and serodoubtful reactions against paratuberculosis. The presence of an insertion sequence IS-900 was detected in 18 samples of udder milk analyzed with the method of direct DNA isolation and in two samples analyzed by means of the culture method.
9

Drobnoustroje chorobotworcze dla czlowieka w mleku surowym [Cz.III]

58%
Ziarno M., Molska I.
Przegląd Mleczarski
|
1999
|
nr 11
386-389
10

Analysis of correlation between the occurrence of anti-MAP antibodies in blood serum and the presence of DNA-MAP in milk

51%
Wiszniewska-Laszczych A., Szteyn J., Smolinska A.
Polish Journal of Veterinary Sciences
|
2009
|
tom 12
|
nr 3
Paratuberculosis (Johne’s disease) is a chronic, infectious enteritis of both domestic and wild ruminants. Unfortunately, the problem of MAP infections is not linked only with the health status of animals and potential direct and indirect economic losses in bovine herds (of dairy cattle in particular). MAP bacilli present in food of animal origin (milk in particular) are likely to lead to the development od the disease in humans. Fast and effective diagnosis of the disease in animals, especially of its subclinical form, may prevent the transmission of the germ to humans. The study was aimed at analyzing the correlations between the occurance of seropositive and serodoubtful reaction in the ELISA test and the presence of DNA-MAP in udder milk. The results suggest that half of the population of animals with positive and doubtful serological responces against John’s disease are likely to be a potential source of germ transmission into humans. The fact of detecting DNA-MAP in 1/3 of all milk samples points to the likelihood of occurrence ofMAP bacilli in milk of animals not displaying seropositive or serodoubtful responses.
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