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The foraging behavior of greater short-nosed fruit bats (Cynopterus sphinx) on wild banana (Musa acuminata) and subsequent dispersal of seeds were studied in the Tropical Rainforest Conservation Area, Xishuangbanna Tropical Botanical Garden, Yunnan province, by direct observation of marked fruits, mist netting, and seed collection. The mean number (± SE) of individual C. sphinx captured by mist net were 2.2 ± 0.33/day and 1.4 ± 0.32/day in the rainy season (September to October) and dry season (November to December), respectively; the difference was not significant. The number of seed pellets expelled was 9.0 ± 1.12/day and 7.2 ± 1.37/day in the rainy and dry seasons respectively; again the difference was not significant. The removal curves for marked fruit were similar for 10 focal trees. Fruits were consumed heavily within two weeks after ripening and all the marked fruit were removed within one month. The difference in seed dispersal was significant between different feeding roosts indicating that patterns of seed dispersal may not be uniform. We found the seeds of M. acuminata can be dispersed by C. sphinx to a distance of about 200 m, and C. sphinx can be considered as an effective seed disperser of M. acuminata.
An efficient protocol was developed for in vitro mass propagation of the commercial cultivar Musa acuminata cv. Dwarf Cavendish through shoot apices culture. Shoot apices with 2-3 pairs of leaf primordia were induced from sliced rhizome explants on Murashige and Skoog (1962) medium supplemented with 6.0 mg/l BA, 150 mg/l Ads and 3% (w/v) sucrose. Multiple shoots were induced from meristematic domes on the same medium. Addition of 1.0-1.5 mg/l IAA to the culture medium and incubation in continuous light (24 h) increased the yield of multiple shoots. The high multiplication coefficient was maintained stable up to the 5th subculture; thereafter it declined. Rooting was readily achieved upon transferring the shoots onto half-strength MS medium supplemented with 500 mg/l activated charcoal and 2% (w/v) sucrose. Micropropagated plantlets were hardened in a polyethylene house and successfully established in soil. There was no morphological variation among the micropropagated plants.
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