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The plasma alpha-l-proteinase inhibitor (API) of three mouse species Mus domes- ticus, M. caroli and M. pahori was isolated. Each of the species isoforms were then separated by chromatofocusing; however, no significant differences in association rate constants toward human neutrophil elastase and bovine chymotrypsin were observed. The amino-acid sequence of the P'i-P'15 C-terminal fragments of the API variants indicate that mouse plasma contains at least two different active API isoforms in the case of M. domesticus (five API genes) but only one active API isoform in M. pahori and M. caroli (one API gene).
Skulls of 892 house mice of five species (Mus Linnaeus, 1758), collected from 136 localities across Europe and Morocco, were studied. The analysis revealed that variations in size affected most of the characters considered, indicating a reed to size-adjust- the data. M. domesticus was morphologically the most variable of all the European mice yet this variability was not consistent with the distribution of sub­species domesticus and brevirostris, The population from Albania was distinct within the M. domesticus samples, resembling M. musculus in overall size. In M. musculus, a W-E gradient of size was found in some variables, especially in females, and a sex dimorphism appeared also in populations from western parts of its range. Among 619 mice from 66 samples across the Czech and Slovak Republics and western Ukraine, but not from populations from western Bohemia, only M. musculus was substantiated. In spite of the fact that M. spicilegus and M. macedonicus are genetically and morpho­logically very close, as many as 9 variables (both untransformed and size-adjusted) proved to be different between the two species while M. spretus was found to be morphologically intermediate.
Maximum cold-induced rate of food consumption in cold-acclimated laboratory house mice Mus domesticus averaged 10.4 g/day in females (body mass before cold exposure = 25.2 g) and 10.7 g/day in males (body mass = 31.7 g); corresponding maximum rates of energy assimilation were 139 kJ/day in females and 144 kJ/day in males. The traits were highly repeatable: the intraclass correlation coefficient for three trials at -10°C was greater than 0.7 for absolute values and greater than 0.6 for values independent of the initial body mass (residuals from ANCOVA). The estimate of repeatability of the mass-independent traits depends on which estimate of body mass (initial or after cold exposure) is used as a covariate in the regression or ANCOVA model. We conclude that the values of maximum cold-induced food consumption and energy assimilation are reliable estimates of the performance of individuals under a specific set of environmental conditions (low ambient temperature, but not limited access to food) and hence may be subject to phenatypic natural or artificial selection.
Three morphological characters were used to depict the position of the hybrid zone between two species of house mice, M. musculus Linnaeus, 1758 and M. domesticus Schwarz et Schwarz, 1943, across a vast area covering countries of the former Yugo­slavia, Albania, Bulgaria and Greece. Quantitative approach based on a morphological index (MI), resembling the hybrid index widely used in allozyme-based genetic studies, was used. The zone crosses Slovenia south of the Sava River, and then follows the Dinaric Mts to Montenegro and northern Albania. Contrary to many previously published results, the zone was found to run parallel with northern borders of Albania and the former Yugoslavian Macedonia, about 150 km north of the Greek border, thus giving its course rather "shallow" appearance at this part of the Balkan Peninsula.
Information on parasites of vertebrates living in terrestrial ecosystems as monitoring tools for heavy metal environmental pollution is scarce. The present study evaluates the potential suitability of the models Rattus rattus/Moniliformis moniliformis and Mus domesticus/Rodentolepis microstoma as promising bioindicator systems for cadmium and lead pollutions under natural conditions. The highest level of cadmium was found in one specimen of M. moniliformis (335.2 ng g−1 wet weight) and the average concentration of Cd in the acanthocephalan was significantly higher than values found in R. rattus liver and kidney tissues. The maximum concentration of lead occurred in one specimen of R. microstoma (567.4 ng g−1 wet weight) and the average concentration of Pb in the cestode was significantly higher than values found in M. domesticus liver, kidney and muscle tissues. The present results allow proposing both models as promising biomonitoring systems to evaluate environmental cadmium pollution (mainly R. rattus/M. moniliformis) and lead contamination (especially M. domesticus/R. microstoma) in terrestrial nonurban habitats.
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