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Salt stress tolerance of methylotrophic bacteria Methylophilus sp. and Methylobacterium sp. isolated from coal mine spoils

100%

Giri D. D., Kumar A., Shukla P. N., Singh R., Singh P. K., Pandey K. D.

Polish Journal of Microbiology

2013

tom 62

nr 3

Two methylotrophic strains of Bina coalmine spoil BNV7b and BRV25 were identified based on physiological traits and 16S rDNA sequence as Methylophilus and Methylobacterium species. The strains exhibited similar carbon utilization but differed in N utilization and their response to the metabolic inhibitors. Methylophilus sp. was less tolerant to salt stress and it viability declined to one tenth within 4 h of incubation in 2M NaCl due to membrane damage and leakage of the intracellular electrolytes as evident from malondiaaldehyde (MDA) assay. In 200 mM NaCl, they exhibited increased superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) activity while in 500 mM NaCl, enzyme activities declined in Methylophilus sp. and increased in Methylobacterium sp. Among exogenously applied osmoprotectants proline was most efficient; however, polyols (mannitol, sorbitol and glycerol) also supported growth under lethal NaCl concentration.

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Methanotrophs responsible for methane oxidation in natural peats from Polesie Lubelskie Region

100%

Szafranek-Nakonieczna A., Stepniewska Z., Woloszyn A., Ciepielski J.

Acta Agrophysica

2012

tom 19

nr 1

The potential of methanotrophic activity (MTA) has been investigated under labo-ratory conditions in three types of peatland profiles: high (H), transition (T) and low (L) originating from Polesie Lubelskie Region. Selected peat samples differed in respect of pH, TOC, von Post index and moisture. The experiment was conducted at natural moisture (198-719 %w/w) with dif-ferent ranges of both, temperature (5, 10 and 20°C) and CH4 enrichment (1 and 5%v/v). The highest MTA (19.69-155.79 mg CH4kg D.W.-1 d-1) was observed at 20°C. Regardless of temperature, MTA was lower (1.38-51.16 mg CH4 kg D.W.-1 d-1) when peat samples were incubated in atmosphere enriched in 1% than in 5% CH4 v/v (4.75-191.26 mg CH4kg D.W.-1 d-1). Strong influence of tem-perature and sampling sites on MTA was also noted. Total DNA was isolated from the most active (20°C, 5% CH4 v/v) peat samples from each site and the PCR (polimerase chain reaction) amplify-ing of genes pmoA (primers A189f/mb661r) and sequence 16S rRNA (primers Type If /Type Ir and Type IIf/Type IIr) specific for methanotrophic bacteria were carried out. Positive results of PCR with primers of pmoA gene after sequencing confirmed that methanotrophs from L point belong to family Methylococcaceae, while 16S rRNA gene sequences from microorganisms inhabiting H peat demonstrated the highest similarity to genus Methylocystis and Methylosinus.

Poli-beta-hydroksymaslan - bakteryjny polimer biorozkladalny

84%

Haber J., Ostafin M., Sokolov A. P.

Postępy Mikrobiologii

1994

tom 33

nr 4

543-562

Ograniczanie wyników

1 Acta Agrophysica

1 Polish Journal of Microbiology

1 Postępy Mikrobiologii

1 Ciepielski J.

1 Giri D. D.

1 Haber J.

1 Kumar A.

1 Ostafin M.

1 Pandey K. D.

1 Shukla P. N.

1 Singh P. K.

1 Singh R.

1 Sokolov A. P.

1 Stepniewska Z.

1 Szafranek-Nakonieczna A.

1 Woloszyn A.

1 2013

1 2012

1 1994

Wyniki wyszukiwania

Salt stress tolerance of methylotrophic bacteria Methylophilus sp. and Methylobacterium sp. isolated from coal mine spoils

Methanotrophs responsible for methane oxidation in natural peats from Polesie Lubelskie Region

Poli-beta-hydroksymaslan - bakteryjny polimer biorozkladalny