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W pracy przedstawiono wyniki oceny sensorycznej oraz hedonistycznej w zakresie smaku i zapachu dla naparów ze świeżych liści mięt. Analizie poddano 7 gatunków i odmian mięt należących do trzech chemotypów (karwonowy, linalolowy i mentolowy), uprawianych w gruncie. W badaniu intensywności smaku i zapachu najwyższą punktację uzyskały: mięta pieprzowa M.× piperita L. (chemotyp mentolowy) oraz mięta marokańska M. spicata L. ‘Moroccan’, (chemotyp karwonowy). Najmniej intensywny był zapach i smak dwóch mięt karwonowych: kędzierzawej (M. crispata L.) i okrągłolistnej (M. rotundifolia Huds.). Uzyskane profile sensoryczne obrazują duże zróżnicowanie badanych mięt pod względem smaku i zapachu. Wykazano podobieństwo pod względem deskryptorów smaku w obrębie poszczególnych chemotypów mięt. W ocenie hedonistycznej największą akceptację uzyskała mięta marokańska, najmniejszą mięta imbirowa (chemotyp linalolowy).
 The essential oils (EOs) and static headspaces (HSs) of in vitro plantlets and callus of Mentha x piperita were characterized by GC-MS analysis. Leaves were used as explants to induce in vitro plant material. The EO yields of the in vitro biomass were much lower (0.1% v/w) than those of the parent plants (2% v/w). Many typical mint volatiles were emitted by the in vitro production, but the callus and in vitro plantelet EOs were characterized by the lack of both pulegone and menthofuran. This was an important difference between in vitro and in vivo plant material as huge amounts of pulegone and menthofuran may jeopardise the safety of mint essential oil. Regarding the other characteristic volatiles, menthone was present in reduced amounts (2%) in the in vitro plantlets and was not detected in the callus, even if it represented the main constituent of the stem and leaf EOs obtained from the cultivated mint (26% leaves; 33% stems). The M. piperita callus was characterized by menthol (9%) and menthone (2%), while the in vitro plantlet EO showed lower amounts of both these compounds in favour of piperitenone oxide (45%). Therefore, the established callus and in vitro plantlets showed peculiar aromatic profiles characterized by the lack of pulegone and menthofuran which have to be monitored in the mint oil for their toxicity.
The use of natural and biodegradable material (such as fish flour) counteracts stress as cheaper and safer alternative for toxic chemicals (such as pesticides). The effect of calcium and fish flour (Ca and FF) (single or in combination) to improve plant tolerance against salt stress was studied. Sterilized mentha seeds were imbibed in each treatment: FF (10 g mL–1), Ca (1, 3%) applied alone and in Ca+FF-combination shaking for 24 h at 150 rpm. Changes in the antioxidants (carotenoids, phenolic, and flavonoid), enzymatic system (superoxidase – SOD, guaiacol-dependent peroxidase – GPX and phenylalanine ammonia-lyase – PAL) and lipid peroxidation levels of mentha seedlings were investigated under salt stress. It was suggested that Ca and/or FF had positive regulation effects on the key enzyme activities related to phenolic compounds biosynthesis and individual phenolic contents under salt stress. Additionally, the mentha plants developed from Ca+FF-combined pre-treatments showed better response to salinity than either Ca or FF single pretreatment. Suppression of salt injury by Ca+FF pre-treatment reduced the LPO levels, increased enzyme activities and promoted total flavonoid and phenolic contents. Ca+FF-combined pre-treatment of mentha seeds seem to be a reliable, not-expensive and easy procedure to enhance plant salt tolerance and to gain more biomass.
Tetranychus urticae (Acari: Tetranychidae) infesting many plants but Mentha viridis L., and Mentha piperita L., were low in number of infestation. Therefore the objective of this study was to identify the resistance of M. viridis and M. piperita plants against T. urticae by studying the external shape and internal contents of those plants. For morphological studies, dried leaves were covered with gold utilizing an Edwards Scan coat six sputter-coater. For histological studies, arrangements of Soft Tissue technique were used. For phytochemical studies, the plants were cut, dried and then high performance liquid chromatography (HPLC) was used. While feeding the mites were collected from the area between oily glands, trichomes and respiratory stomata in both mint species. The most important leaf structures in aromatic plants are the oily glands found on the external part of the leaves (both upper and lower epidermis). The number of oil glands in M. viridis leaves was greater than in M. piperita; the trichomes on the epidermis of M. viridis were greater in number than in M. piperita; the spongy mesophyll in M. viridis was much thicker than in M. piperita. The essential oils in the leaves of both mint species contained 71 compounds representing 99.61% of the total oil constituents identified from M. viridis before infestation, and 90.95% after infestation, and about 99.65% from M. piperita before infestation, and 99.98% after infestation.
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