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The aim of this research is a genetic mapping of the STS markers derived from a model legume Medicago truncatula Gaertn. in lupin crop (Lupinus angustifolius L.). This study was undertaken within the framework of VI EU „Grain Legumes Integrated Project” (GLIP). The mapping population consists of 89 recombinant inbred lines of a cross between a domesticated line and a wild type (83A:476 x P27255). STS markers were generated by the PCR, using primer pairs designed on the basis of M. truncatula and P. sativum genomic sequences. Up till now 257 primer pairs designed and delivered by the Hungarian partner of the project have been tested. Most of the primers amplified a monomorphic product. In the case of 29 detected polimorphic products, segregation of alleles could be analyzed in the whole mapping population. 22 of these markers were located on the genetic linkage map based on the MFLP markers constructed by the Australian group. The position of the remaining 6 markers is still uncertain. Supplementing the published map with the STS markers increased its length to 1953 cM and the average marker distance into 4.1 cM.
 Annexins belong to a family of multi-functional membrane- and Ca2+-binding proteins. The characteristic feature of these proteins is that they can bind membrane phospholipids in a reversible, Ca2+-dependent manner. While animal annexins have been known for a long time and are fairly well characterized, their plant counterparts were discovered only in 1989, in tomato, and have not been thoroughly studied yet. In the present review, we discuss the available information about plant annexins with special emphasis on biochemical and functional properties of some of them. In addition, we propose a link between annexins and symbiosis and Nod factor signal transduction in the legume plant, Medicago truncatula. A specific calcium response, calcium spiking, is an essential component of the Nod factor signal transduction pathway in legume plants. The potential role of annexins in the generation and propagation of this calcium signal is considered in this review. M. truncatula annexin 1 (MtAnn1) is a typical member of the plant annexin family, structurally similar to other members of the family. Expression of the MtAnn1 gene is specifically induced during symbiotic associations with both Sinorhizobium meliloti and the mycorrhizal fungus Glomus intraradices. Furthermore, it has been reported that the MtAnn1 protein is preferentially localized at the nuclear periphery of rhizobial-activated cortical cells, suggesting a possible role of this annexin in the calcium response signal elicited by symbiotic signals from rhizobia and mycorrhizal fungi.
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