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1

Wplyw stresu transportowego na stopien wrazliwosci leukocytow na dzialanie leukotoksyny Mannheimia haemolytica

100%
Urban-Chmiel R., Puchalski A., Wernicki A.
Medycyna Weterynaryjna
|
2006
|
tom 62
|
nr 09
1045-1047
The purpose of the study was estimating the viability and susceptibility effect of leukocytes isolated from cattle before and after the transportation in vitro on M. haemolytica leukotoxin (Lkt) cytotoxicity. 40 Simentaler heifers that were transported by truck a distance of 1700 km for 72 hours were used in the experiment. The material for the study was the blood (40 samples) collected on heparin directly before and after transportation. In relation to leukocytes the examination of susceptibility on cytotoxic effect of Lkt has been carried out with the use of MTT (microtitration assay) and the viability of leukocytes after 1, 2, 3 and 6 hour of incubation. The results obtained in the cell viability test did not show statistically significant differences (P≥0.05) in 1st and 2nd hour of incubation in leukocytes obtained from heifers before and after transportation. After the 1st hour of incubation the percentage of leukocyte viability was on a very high level and showed 87% in both groups of animals. The significantly lower cell viability values in comparison to leukocytes isolated from animals before the transportation was observed in the transported heifers from 3rd to 6th hour of incubation. The analysis of the results obtained by MTT test indicated statistically significant differences in the susceptibility of leukocytes for cytotoxic activity of Lkt. The average values of toxic activity of Lkt in relation to leukocytes isolated before and after transportation was 79% and 92% respectively. The lytic activity of Lkt for 50% of the cell population referred as 1 unit (1U) was observed in Lkt concentration 15 mg/ml (leukocytes before transportation) and 7.5 mg/ml (after the transportation). The increase of susceptibility of leukocytes isolated after transportation on the cytotoxic effect of leukotoxin suggest the significant influence of transporting stress on the increase of respiratory diseases caused by M. haemolytica strains.
2

Wplyw podwyzszonej temperatury na ekspresje bialek szoku termicznego [Hsp70] u terenowych szczepow Mannheimia haemolytica serotyp 1

100%
Urban-Chmiel R.
Medycyna Weterynaryjna
|
2006
|
tom 62
|
nr 07
801-803
The purpose of the study was to estimate the influence of temperature increase on Hsp70 induction in M. haemolytica serovar 1 strains. Three wildtype M. haemolytica strains, obtained from calves respiratory tracts and incubated at a temp of 41.5°C for 2 hours were used as the research material. Analyses of particular fractions were carried out by SDS-PAGE electrophoresis and identification of obtained proteins by immunoblotting (Western blotting) using polyclonal rabbit anti Hsp70 antibodies. The first step was to separate the capillaries in gradient pH 5/7 and 3/10 which was carried out in two-dimensional electrophoresis. The second step was carried out in SDS-PAGE electrophoresis using 4% stocking and 12% resolving gels. An analysis of SDS-PAGE electrophoresis revealed additional protein fractions, displaying positive reactions with anti-Hsp70 antibodies. The presence of these proteins was observed both in membrane and cytoplasmatic bacterial cell fractions. The molecular weight of the obtained proteins ranged between 77.5-79 kDa. The additional protein fractions were present in membrane fractions between molecules, weighed 22-26 kDa, as well as displaying a positive reaction with anti-Hsp70 antibodies. The electrophoregrams obtained in 2D electrophoresis revealed the presence of additional spots in membrane, cyto- and periplasmatic fractions. The obtained results suggest the potential for M. haemolytica strains to produce Hsp70 during stress induced by temperature increase.
3

Evaluation of peripheral blood leukocyte subpopulations by flow cytometry in calves treated with Mannheimia haemolytica leukotoxin

100%
Bednarek D., Urban-Chmiel R., Dudek K., Szymanska-Czerwinska M.
Bulletin of the Veterinary Institute in Puławy
|
2009
|
tom 53
|
nr 2
199-203
The aim of the study was to evaluate the effect of Mannheimia haemolytica leukotoxin (Lkt) on cellular immune response in clinically-healthy calves given intravenously 25 µg per animal of M. haemolytica Al Lkt. The alternations of peripheral blood leukocytes were examined with a flow cytometry. The Lkt treated calves were compared with the non-treated controls before (0) and 1, 2, 3, 4, 5, 6, and 24 h after the treatment. The following parameters were assayed: white blood cell (WBC) count, percentage of polymorphonuclear leukocytes (PMNL), mid-size leukocytes, total percentage of lymphocytes and their subsets: CD2⁺ (T lymphocytes), CD4⁺ (T helper lymphocytes), and CD8⁺ (T suppressor/cytotoxic lymphocytes) with CD4⁺: CD8⁺ratio, and also WC4⁺ cells (B lymphocytes). The obtained results showed that in the treated calves, the WBC count, the percentages of PMNL, mid-size leukocytes, and some subpopulations of peripheral lymphocytes (CD2⁺, CD4⁺, CD8⁺) were significantly lower compared with the controls during the first of three hours of the experiment, and then the parameters increased and returned to the physiological level.
4

Evaluation of Mannheimia haemolytica leukotoxin prepared in nonsupplemented and BSA or FBS supplemented RPMI 1640 medium

100%
Urban-Chmiel R., Wernicki A., Puchalski A., Mikucki P.
Polish Journal of Veterinary Sciences
|
2004
|
tom 07
|
nr 1
Mannheimia haemolytica leukotoxin (Lkt) is the major factor that contributes to lung injury in bovine pneumonic pasteurellosis. Supernatant preparations containing Lkt produced by M. haemolytica serotype 1, grown in RPMI 1640 medium supplemented with BSA or FBS and without supplements were evaluated during this study. Analysis of obtained Lkt showed presence of 105kDa antigen (SDS-PAGE electrophoresis). The obtained bacterial protein fraction estimated as Lkt was detected by Western blotting with mouse monoclonal (Mab 605 and Mab 601) anti-Lkt antibodies. No significant differences were found in obtained leukotoxin between wildtype and reference M. haemolytica strains. Our studies showed that growth in media supplemented with BSA or FBS had no significant influence on leukotoxin production. When BSA or FBS supplements were used, additional protein fractions in electrophoregrams SDS-PAGE were observed. These protein bands did not react with Mab 605 and/or Mab 601 in Western blotting analysis. Lkt immunogenicity was detected by immunoblotting with sera from Lkt immunized rabbits and calves.
5

Field trial of primary and booster dose of inactivated vaccine against bovine respiratory bacteria in young Holstein calves

84%
Mori K., Kato T., Yokota O., Ohtsuka H.
Journal of Veterinary Research
|
2020
|
tom 64
|
nr 2
6

Skutecznosc natywnej i inaktywowanej leukotoksyny Mannheimia haemolytica w immunoprofilaktyce swoistej syndromu oddechowego owiec

84%
Mikucki P., Wernicki A., Puchalski A., Urban-Chmiel R.
Medycyna Weterynaryjna
|
2006
|
tom 62
|
nr 11
1302-1305
The purpose of this study was to compare the immunostimulating properties and prophylactic effects of native M. haemolytica leukotoxin (Lkt) which was inactivated by formaldehyde and glutaraldehyde. The study also estimated the neutralizing properties of antibodies induced by different forms of Lkt and their protective effects on experimental challenge in sheep. The results of the study suggest that the inactivation procedure has an important effect on immunogenic obtained toxins. Vaccines used in the immunized animals demonstrated an immunogenic presence which was observed in an increase of the specific antibody titers in sera following immunization. An increase in the neutralizing properties of antibodies contained in the sera of animals immunized by Lkt was also observed. The obtained results indicate the possibility of a more effective use of M. haemolytica Lkt isolated from bovine respiratory syndrome as a subunit of the vaccines already used in immunoprophylaxis of this disease in sheep.
7

Mannheimia haemolytica a syndrom oddechowy bydła (BRD)

84%
Dudek K., Bednarek D.
Weterynaria w Terenie
|
2015
|
tom 09
|
nr 3
8

Wykorzystanie bakteriofagów w zwalczaniu zakażeń wywoływanych u bydła przez M. haemolytica

84%
Urban-Chmiel R., Wernicki A., Stegierska D., Dudzic A., Puchalski A., Dec M.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2014
|
tom 09
|
nr 2 Monografia
9

Serum vitamin E and malondialdehyde concentrations in experimentally induced Mannheimia [Pasteurella] haemolytica infection in ewes fed dam milk or cow milk after birth

84%
Orbay A. E., Altiner A.
Bulletin of the Veterinary Institute in Puławy
|
2007
|
tom 51
|
nr 2
Twenty Chios newborn ewe lambs were used. Ten lambs were fed ad libitum colostrum and dam milk (group 1). The other ten lambs were separated from their dams immediately after birth and were not allowed to suckle normally, but were fed commercial cow's milk with a feeding bottle (group 2). When they were one year old, M. haemolytica serotype Al (10⁹ germs) was inoculated intratracheally to each ewe. Serum vitamin E and malondialdehyde (MDA) concentrations were determined in all the sheep before inoculation, and on days 1, 10, and 22 after inoculation, in addition to day 7 after antibiotic treatment. Serum vitamin E concentrations significantly decreased on day 1 after bacterial inoculation in group 1, rose on day 10 and continued to rise on day 22 after inoculation. The concentrations of the compounds did not significantly differ from the values observed on day 22 after antibiotic administration (on day 7 after treatment). Vitamin E concentration significantly decreased on day 1 after inoculation in group 2, and significantly rose on day 10 and then remained constant. Significant differences were observed between groups 1 and 2 before inoculation (P≤0.05). While serum vitamin E concentration of group 1 was higher than group 2 before inoculation, the concentrations in group 2 were significantly higher than in group 1 on days 10 and 22 after inoculation. Serum MDA concentrations gradually significantly increased in groups 1 and 2 after bacterial inoculation (P≤0.05), and then decreased to the pre-inoculation values after antibiotic treatment. No significant difference was observed between group 1 and group 2 on any sampling day. In conclusion, M. haemolytica infection induced lipid peroxidation associated with a rapid consumption of the antioxidant vitamin E (day 1), following by mobilisation of liver vitamin stores, probably more intense in ewes fed artificially after birth. The similar serum MDA concentrations in the two groups suggests that, a resistance against an infection can develop in sheep fed with artificially cow's milk within one year, but the effects of repeated infections on such animals still remains to be explored.
10

The influence of transport stress on the humoral immunological response of calves induced by Mannheimia haemolytica leukotoxin

84%
Wernicki A., Urban-Chmiel R., Mikucki P., Puchalski A., Kankofer M.
Polish Journal of Veterinary Sciences
|
2003
|
tom 06
|
nr 1
In the contemporary systems of cattle production, transport stress is the most essential polyetiological factor responsible for inducing unfavourable reactions in the animals. The main reason for this phenomenon is the immunosuppressive effect of steroid hormones on cellular and humoral protective mechanisms. The purpose of the present study was to establish the relationship between the Cortisol concentration as an indicator of the stress reaction occuring directly after the transportation of calves and the specific humoral immune response to the leukotoxin (Lkt) antigen produced by the M. haemolytica strain. The experiment was carried out on 19 clinically healthy calves, weighing about 100 kg and transported by track for about 2 hours. After the delivery of the animals for feeding to the traditional cattle-house, the calves were immunized s.c.: group I with 1 ml Lkt (in conc. - 10 μg/ml) with 1 ml of adjuvant on the 1st and 14th day after the transportation, group II with the same Lkt doses on the 3rd and 16th day after the transportation. The animals of the control group were vaccinated on the 1st and 14th day after the transportation with the twice diluted adjuvant. In examined sera the Cortisol concentrations and the level of Lkt antibodies were measured by ELISA test. The cytotoxin neutralizing (CN) antibody level (cytotoxity assay) was determined with a simple visual assay. The study revealed, significant differences (P ≤ 0.01) in serum Cortisol leves between the control and experimental animals. The analysis of the absorbance of the sera in both groups immunized with Lkt showed substantial differences (P ≤ 0.05) from the 6th through to 22nd day of the experiment compared with the control group. The analysis of the results of CN antibody titers showed no differences between the sera from group I and II. Based on the results obtained in this experiment it can be assumed, that a short transportation stress has no important influence on the level of specific humoral anti-Lkt response.
11

Effect of experimental Mannheimia haemolytica infection in sheep fed colostrum and dam or cow milk after birth on serum IgG

84%
Altiner Y., Ozpinar A., Ulutas P. A., Erhard M.
Bulletin of the Veterinary Institute in Puławy
|
2005
|
tom 49
|
nr 4
12

Mannheimia haemolytica od bakterii komensalicznej do zabójcy bydła

71%
Bayron C.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2018
|
tom 13
|
nr 1
13

Zespół sercowo-płucny bydła

67%
Dudek K., Bednarek D.
Weterynaria w Terenie
|
2015
|
tom 09
|
nr 4
14

Effect of leukotoxin of Mannheimia haemolytica and LPS of E. coli on secretory response of bovine neutrophils in vitro

67%
Wessely-Szponder J., Urban-Chmiel R., Wernicki A., Bobowiec R.
Polish Journal of Veterinary Sciences
|
2005
|
tom 08
|
nr 2
15

Odpowiedź immunologiczna cieląt immunizowanych szczepionką na wpływ leukotoksyny (LKT) Mannheimia haemolytica A1

67%
Bednarek D., Dudek K., Urban-Chmiel R.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2008
|
tom 03
|
nr 2
16

Szczepienia ochronne przeciwko zakażeniom wywoływanym przez M. haemolytica i ich znaczenie w profilaktyce syndromu oddechowego bydla

67%
Dudek K., Bednarek D., Szymanska-Czerwinska M.
Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii
|
2009
|
tom 04
|
nr 2 Monografia
17

Wplyw stresu transportowego na stopien podatnosci bydla i owiec na oddzialywanie leukotoksyny Mannheimia haemolytica

67%
Urban-Chmiel R.
Rozprawy Naukowe. Akademia Rolnicza w Lublinie
|
2007
|
nr 323
1-88
18

'Grypa cielat'. Czesc 2

67%
Rypula K., Karczmarczyk R.
Hoduj z Głową
|
2005
|
nr 4
38-40
19

Paleczki z rodziny Pasteurellaceae. Rola w etiopatogenezie syndromu oddechowego bydla

67%
Bednarek D.
Weterynaria w Terenie
|
2008
|
tom 02
|
nr 3
6-8
20

Comparison of immunogenic properties of native and inactivated Mannheimia haemolytica Lkt in calves

67%
Wernicki A., Mikucki P., Urban-Chmiel R., Puchalski A.
Polish Journal of Veterinary Sciences
|
2008
|
tom 11
|
nr 2
83-88
The aim of this study was to compare the immunostimulatory properties of Lkt of M. haemolytica inactivated by formaldehyde and glutaraldehyde and to evaluate the neutralizing properties of anti-Lkt antibodies. The experiment was conducted on 20 Black-and-White Lowland calves of 100 kg body weight, assigned to 4 experimental groups. The animals were given subcutaneous vaccine injections with native Lkt, Lkt inactivated by formaldehyde or Lkt inactivated by glutaraldehyde. The anti-Lkt antibody titres were measured using an enzyme-linked immunosorbent assay (ELISA), based on absorb- ance of the sera obtained from the animals immunized with the different forms of Lkt. The protective effects of the antibodies present in the sera isolated from the vaccinated animals were estimated using an MIT assay. Analysis of the ELISA absorbance values in the sera from calves in the vaccinated groups did not show any significant differences between the groups. The highest increase in absorbance of sera was observed in calves from the group that received formaldehyde-inactivated Lkt. In the case of calves immunized with native Lkt, the absorbance values were lower than in the group immunized with Lkt inactivated by formaldehyde. The lowest absorbance values were observed in sera obtained from calves vaccinated with Lkt inactivated by glutaraldehyde. Analysis of the MTT assay results revealed the greatest Lkt-neutralizing properties of antibodies in the sera of calves immunized with two doses of a vaccine containing native Lkt and Lkt inactivated with formaldehyde.
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