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Studies on induced physical and chemical mutagenesis in groundnut (Arachis hypogia)

100%

Gunasekaran A., Pavadai P.

International Letters of Natural Sciences

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2015

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tom 08

Mutation breeding has been widely used for the improvement of plant characters in various crops. It is a powerful and effective tool in the hands of plant breeders. In any mutation breeding program, selection of an effective and efficient mutagen is very essential to produce high frequency of desirable mutation. Groundnut (Arachis hypogia) var. VRI-2. was treated with different concentration of physical and chemical mutagen namely gamma rays 10, 20, 30, 40, 50 and 60 KR and Ethyl methane sulphonate (EMS). For inducing mutation various concentration of EMS such as 0.1, 0.2, 0.3, 0.4, 0.5 and 0.6 % for six hours were applied to 200 seed sample of each concentration and one respective control. The LD50 value was observed in 50% of gamma rays and 0.5 % of EMS. The morphological and yield characters were significantly reduced seed germination, seedling survival, days to first flower, plant height, number of leaves per plant, number of grains per plant, grain length and breath, 100 grains weight, grain yield per plant, fresh and dry weight per plant. The increasing doses/concentration of gamma rays and EMS decreased in phenotypic and yield characters in M1generation. The mutagenized populations showed significantly higher variability in the M2 generation. Mutant lines showing higher yield per plant than the respective parents and checks were isolated in M2 and subsequent generation were significantly more pod yield and yield components than the untreated plants.

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Effect of gamma rays in induced morphological mutants on M2 generation of chilli (Capsicum annuum L.) var K1

84%

Mullainathan L., Aruldoss T.

International Letters of Natural Sciences

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2015

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tom 03

The present study was carried out to induce morphological mutation in chilli (Capsicum annuum L.) var k1. The seeds were subjected to different treatment levels of gamma rays are 20, 30, 40, 50, 60 and 70Kr along with control. The treated and untreated seeds were raised in a randomized block design to observe the different morphological characters in M2 generation. Several unique and interesting mutants were isolated in this investigation, these independent mutants have differ from control, the most distinct mutants included tall, dwarf, bushy , flower, sterile and pod mutant, was observed in M2 generation.

3

Studies on effect of physical and chemical mutagens in sorghum (Sorghum bicolor (L.) Moench) in M2 generation

84%

Suthakar V., Mullainathan L.

International Letters of Natural Sciences

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2015

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tom 37

Seeds of Sorghum (sorghum bicolor (L.) Moench) Variety CSV – 23. Were mutagenesis with gamma rays and EMS to determine their mutagenic sensitivity in morphological and yield characters. The spectrum of Mutation and induced variability for various quantitative and yield traits were observed in M2 generation. Plant height (cm),total number of leaves, leaf length (cm), leaf width(cm), stem girth (cm),Days taken to first flowering (days),ear head length(cm),ear head width(cm), Grain weight(gm),100 seed weight(gm), Biomass weight(gm).

4

Effect of mutagens on quantitative characters in M2 and M3 generation of sesame (Sesamum indicum L.)

67%

Ravichandran V., Jayakumar S.

International Letters of Natural Sciences

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2015

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tom 42

The mutagenic effects of different dose/concentrations of gamma rays (30, 40 and 50 KR) and ethyl methane sulphonate (1.0, 1.5 and 2.0 mM) on sesame (Sesamum indicum L.) varieties VRI-1 were investigated. The characters studied include; days to first flower, plant height, number of branches per plant, number of capsule per plant, number of seeds per capsule and seed yield per plant in M2 and M3 generations. Both negative and positive shifts in mean values were recorded as a result of the physical and chemical treatments. The results indicate the possibilities of evolving higher yield variants through proper selection. Thus, economic traits like number of capsule per plant, number of seeds per capsule and hundred seed weight in M3 generation offer scope for selection and improvement.

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Frequency and spectrum of induced viable macromutations in chickpea (Cicer arietinum L.) cultivar ‘Vishwas’

67%

Barshile J. D.

International Letters of Natural Sciences

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2015

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tom 03

Present investigation was undertaken to study the frequency and spectrum of induced viable macromutations employing SA, EMS and gamma radiation in chickpea (Cicer arietinum L.) cultivar Vishwas (Phule G 5). The seeds of chickpea, cultivar Vishwas were treated with three different concentrations / doses of SA (2, 3 and 4 mM), EMS (8, 12 and 16 mM) and gamma radiations (400, 500 and 600 Gy). The mutagen administered seeds were sown in experimental fields to raise M1 progeny. Seeds of M1 plants and control were harvested separately and sown to raise M2 population. The M2 progeny were screened for viable macromutaions. A wide spectrum of viable macromutations was isolated in the M2 generation. In all twenty four different types of viable morphological macromutations were observed. These included 7 types of plant type mutations and 6 types of leaf mutations, 1 types of flower mutation, 5 types of pod mutation and 5 types of seed mutations. Results indicated that all mutagenic treatments were effective in inducing viable mutations in chickpea, during M2 generation. Differences in response to different mutagens were observed in the spectrum and frequency of viable mutations. Some mutation types occurred more frequently than others. The frequency and spectrum of viable mutations were relatively high with EMS followed by gamma radiation and SA. In the present investigation, an attempt has been made for increasing frequency and spectrum of locally important chickpea cultivar ‘Vishwas’ employing chemical and physical mutagens.

6

Selection of winter rye genotypes containing enhanced levels of aspartate family amino acids

67%

Dziadczyk P., Smiech M., Malepszy S.

Journal of Applied Genetics

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1996

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tom 37A

7

Wplyw mutagenow chemicznych na cechy morfologiczne u petunii [Petunia x atkinsiana D.Don]

59%

Krupa-Malkiewicz M.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

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2009

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nr 251

305-314

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Wielocechowa analiza pokolen M1 i M2 jeczmienia jarego uzyskanych po dzialaniu MNU i gibereliny

51%

Rybinski W.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

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1996

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tom 200

185-189

Ograniczanie wyników

5 International Letters of Natural Sciences

2 Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

1 Journal of Applied Genetics

2 Mullainathan L.

1 Aruldoss T.

1 Barshile J. D.

1 Dziadczyk P.

1 Gunasekaran A.

1 Jayakumar S.

1 Krupa-Malkiewicz M.

1 Malepszy S.

1 Pavadai P.

1 Ravichandran V.

1 Rybinski W.

1 Smiech M.

1 Suthakar V.

5 2015

1 2009

2 1996

Studies on induced physical and chemical mutagenesis in groundnut (Arachis hypogia)

Effect of gamma rays in induced morphological mutants on M2 generation of chilli (Capsicum annuum L.) var K1

Studies on effect of physical and chemical mutagens in sorghum (Sorghum bicolor (L.) Moench) in M2 generation

Effect of mutagens on quantitative characters in M2 and M3 generation of sesame (Sesamum indicum L.)

Frequency and spectrum of induced viable macromutations in chickpea (Cicer arietinum L.) cultivar ‘Vishwas’

Selection of winter rye genotypes containing enhanced levels of aspartate family amino acids

Wplyw mutagenow chemicznych na cechy morfologiczne u petunii [Petunia x atkinsiana D.Don]

Wielocechowa analiza pokolen M1 i M2 jeczmienia jarego uzyskanych po dzialaniu MNU i gibereliny