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The manuscript describes an easy method of isolation of plant genomic DNA. This method allowed us to isolate substantial amounts of good quality DNA from lupin (Lupinus luteus) tissues. The described method also appeared to be useful for genomic DNA isolation from tissues of other plants.
The paper presents an analysis of the impact of meteorological factors (solar radiation, maximum, minimum and mean temperature, precipitation) on the development and yield of yellow lupin Parys cultivar in the northern Poland in the years 1987–2008. Using multiple regression methods (linear and quadratic function) created regression equations that were estimated using the coeffi cients of determination (R2, R2 adj and R2 pred – using the Cross Validation procedure). Selected regression equation used to estimate the yield of yellow lupin, using generated – by means of model WGENK – daily values of global radiation, maximum and minimum temperature, precipitation, and climate change scenario GISS Model E for Central Europe. Examined dependencies weather-yield of lupine seeds (cultivar Parys) allowed the application of the chosen model to forecast yields from the time when the values are independent variables in the model by the end of the growing season. The comparison of distributions of actual and simulated yields shows that real yields are slightly (by 0.06 t·ha–1) higher than those generated for 2 × CO2 conditions.
The lupine (Lupinus luteus cv. Ventus) cDNA clones encoding homologues of cyclin (CycBlfi, CycBl;3, CycBl;4) have been Isolated from cDNA library pre­pared from roots inoculated with Bradyrhizobium lupint. Comparison of the deduced amino-acid sequences of CycBlg, CycBl;3, CycBl;4 and previously described CycBl;l (Deckert et al 1996, Biochimie 78, 90-94) showed that they share 46-65% of identical amino acids. The presence of conserved residues (Renaudin et al., in The Plant Cell Cycle, in the press; Renaudin et al.. Plant Mol. Biol., in the press) along with phylogenetic analysis of known plant cyclins revealed that the four lupine sequences belong to subgroup I of B-like mitotic cyclins.
Flavonoid glycosides constitute important group of plant secondary metabolites. This class of natural products play significant role in different physiological processes. A new methodological approach where mass spectrometric techniques are applied to structural studies of this class of compounds is presented. Four flavonoid O-monoglycosides and one C-monoglycoside were isolated from green parts of lupin (Lupinus luteus L.). Several different mass spectrometric techniques were applied to structural elucidation of isolated compounds. Desorption ionization mass spectrometry was used for registration of mass spectra of intact and derivatized (permethylated) flavonoid glycosides. In some cases electron impact mass spectra of permethylated compounds were also recorded. Methylated samples after methanolysis and further derivatization of free hydroxyl groups (methylation or acetylation) were analyzed with gas chromatography-mass spectrometry. Combined information drawn from the registered mass spectra enabled us to define molecular mass, structure of aglycones and sugars, and positions of glycosidic bonds on the aglycon. Structures of four flavonoid monoglycosides were elucidated as follows: genistein 7-O-glucoside (1), genistein 4'-O-glucoside (2), 2'-hydroxygenistein 7-O-glucoside (3), and apigenin or genistein 8-C-glycoside (5). For the fourth O-glycoside (4) only molecular mass and masses of the aglycone and sugar were estimated.
A strict two-factor field experiment was set up as a randomized complete block design over 2000-2002 at the Experimental Station of the Faculty of Agriculture, University of Technology and Agriculture, at Mochełek, and involved a traditional yellow lupin cultivar, `Idol´, whose plants were sprayed with Tytanit preparation, containing 46% of Ti. The first factor covered the following objects: three spraying dates: single spraying after blossom fall, double spraying - after blossom fall and 7-10 days later and triple spraying - after blossom fall, 7-10 days later and then 7-10 days later. The second factor involved the following objects of Tytanit concentration: 0.02, 0.04 and 0.06%. The plots which were not sprayed constituted the control. Throughout the research years there was observed a significant effect of Tytanit on 7.4-18% increased yellow lupin seed yield. Tytanit did not show a significant effect on the structural components of the seed yield from the main stem; its application resulted in a significant, as compared with the control, increase in the number of pods and seeds as well as in the seed weight from branches. As for yellow lupin yielding, the optimal solution seems to be offered by a double application of 0.02-0.04% of Tytanit straight after plant blossom fall and 7-10 days later.
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