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Invertase and alpha-glucosidase production by the endemic Antarctic marine yeast Leucosporidium antarcticum

100%

Turkiewicz M., Pazgier M., Donachie S. P., Kalinowska H.

Polish Polar Research

2005

tom 26

nr 2

125-136

The marine psychrophilic and endemic Antarctic yeast Leucosporidium antarcticum strain 171 synthesizes intracellular ß-fructofuranosidase, and intra- and extracellular α-glucosidases. Each enzyme is maximally produced at 5°C, while the strain's optimum growth temperature is 15°C. Invertase biosynthesis appeared regulated by catabolic repression, and induced by sucrose; the enzyme was extremely unstable ex vivo, and only EDTA, Mn2+, and BSA stabilized it for up to 12 h after yeast cell lysis. Thermal stability of the invertase was also low (30 min at temperatures up to 12°C). The optimum temperature for invertase activity was 30°C, and optimum pH was 4.55 to 4.75. The extracellular α-glucosidase was maximally active at 35°C and pH 6.70-7.50, and stable for 30 min up to 20°C.

Ograniczanie wyników

1 Polish Polar Research

1 Donachie S. P.

1 Kalinowska H.

1 Pazgier M.

1 Turkiewicz M.

1 2005

Wyniki wyszukiwania

Invertase and alpha-glucosidase production by the endemic Antarctic marine yeast Leucosporidium antarcticum