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This is the first study on spatial behaviour of brown hares Lepus europaeus Pallas, 1778 based on radio-telemetry in a natural system, which we contrast with data from agricultural systems. Radio tracking took place in a Dutch salt marsh over a 10-month period, with intensive tracking sessions during April/May and December/January. Six hares could be followed in both periods and in total 1224 fixes were collected. Average home range size was calculated as 28.7 ± 8.5 ha when using Adaptive Kernell method (Mimimum Convex Polygon: 27.3 ± 9.0 ha) on 90% of all fixes. Such values are in the lower end of the range of those obtained for agricultural systems. Home range size did not differ between sexes, day and night, or across seasons. However, the size of the core range (50% of fixes) was twice as large in May compared to the winter period, and thus inversely related to food availability. Unlike in agricultural systems, use of space by hares did not change over the course of the season. This probably reflects the patchy nature of the natural habitat which provides food and shelter throughout the year in a confined area.
We have studied fluctuating asymmetry (FA), as indicator of developmental stability, and between-individual variation, as surrogate of developmental canalization (DC), in long bones (humerus, ulna, radius, femur, tibia) of 72 wild-living adult-sized brown haresLepus europaeus Pallas, 1778 with variable individual heterozygosity (H).H was calculated from 13 polymorphic allozyme loci. According to the “over-dominance hypothesis”, we expected increased developmental stability and canalization at higherH-levels. But at the individual level we did not find any significant correlation between overall FA (FAI) andH. Also, standard deviations (SD) of mean length (over both body sides) of bones did not differ between individuals from two intentionally created groups of hares, namely one with high and one with lowH. FA-indices and variances of FA-indices of bone lengths did not differ significantly when compared between two intentionally created groups of hares with high and low SD of bone lengths, respectively. These latter findings suggest that developmental stability and DC are two separate or partly separate mechanisms of developmental homeostasis in the studied appendicular skeleton, and thatH has no traceable effect on develop-mental homeostasis. If there is still such an effect, it should be clearly smaller than a possibly combined effect of (presently uncontrolled) environmental stressors.
In order to assess the reasons for a 60% decline of the bag record of hares in Denmark during the last three dccades, reproduction in Lepus europaeus Pallas, 1778 was studied in a location in Funen (Denmark) during April 1984 - March 1987. The breeding season was initiated around 1 January and lasted until September - October. In 1985 and 1986, 18.17c and 25.1% of the females delivered four litters, while three litters was maximum in 1984; but 13.6% - 21.4% of the females did not breed at all. The average number oflitters produced per adult female was 1.93, 2.54, and 2.51 in 1984, 1985 and 1986, respectively; average litter sizes were 2.11, 2.33 and 2.06 in the survey years. For the whole study period, the average sizes of litters 1-4 were 1.51, 2.54, 2.53 and 1.71, respectively. During the shooting seasons the hare bags in the study area indicated 1.27, 1.64 and 1.01 young per female shot jn 1984, 1985 and 1986, respectively. Postnatal mortality was calculatcd to be 68.0%, 72.3% and 80.6% in 1984, 1985 and 1986, respectively. The results indicate a relatively poor reproductive success due to a poor production of young and a high postnatal mortality. Shortage of sufficient nutrients in modern farming systems during the mid summer period may be a plausible explanation of these findings.
In 1987 - 1990 an investigation was made on the spatial structure of brown hare Lepus europaeus Pallas, 1778 populations using the transect routes method. The in­vestigation was carried out in two different parts of Poland that differed in the charac­ter of agriculture: 1 - northeastern with large fields of state farms, and II - east- central with small fields of individual farmers. The results indicate that independently of the agrarian structure, the distribution of hares in crops corresponds to their per­centage share in the region, with exception of rape fields, which show a tendency to be avoided. There was also a significant relationship between the spatial distribution of the hare and the habitat diversity - highly variegated fields are more consistently inhabited by hares. Besides, hares show a distinct tendency to prefer areas close to the field margins. Positively oblique distance distributions between the individuals and the margins of field crops are particularly strongly expressed in the region of a highly monotonous crop species. It may be expected that a change in type of farming by passing to the large-fields and continuous cropping is one of the factors that make it difficult for the hare to utilize the space within the habitat.
We describe the results of our research on population dynamics among brown hares reared in enclosures and then released into suitable natural habitat. Radio-tracking was used to follow the fate of 60 released brown hares over a 4-year period, extending between November 2005 and November 2009. The survival rate among these animals after 12 months was estimated to be 37 %, with 22 tagged individuals surviving beyond 1 year post-release. The highest (40 %) level of mortality characterised the first month after release, while a second period of enhanced mortality coincided with the breeding season (altogether accounting for a 20 % mortality rate). There was no significant relationship between body mass and mortality rate in the first month following release. A natural cause of death was predation by mammals, which accounted for some 31 % of all losses. Remaining causes were poaching (13 %), hits by vehicles (7 %) and unidentified causes (9 %). However, in at least 40 % of cases, it was not possible to determine the date when a released animal died, to say nothing of the cause of death.
The paper presents the selected indicators of hare populations in Poland. The material consisted of 528 hares acquired during traditional hunts. For each hunted hare we specified its weight, sex and age. All individuals were classified into two age groups: the first comprised juveniles (up to 12 months of age), while the other – adults (over 12 months). The ratio of young individuals in relation adults varies a lot (26−56%). The males to females ratio was approximately 1:1 with a slight advantage in favor of females. Reproductive rate was between 0.36 and 1.26, while reproductive success – 0.7−2.7. Average weight of hunted hares ranged between 3.42 and 4.32 kg. No significant differences in body weight were noticed within gender groups (Z=1.298, p>0.05, Mann−Whitney U test). However, they were found within the age groups (Z=–9.657, p <0.05, Mann−Whitney U test).
The aim of the study was to evaluate the parameters of genetic variability at the MHC class II DRB1 and DQA1 loci in 112 brown hares from 4 regions of Poland. Disturbances in the Hardy-Weinberg (HW) equilibrium associated with an increase in homozygocity at these loci may lead to an increase in the proportion of females whose fetuses have compatible MHC haplotypes. This compatibility may result in abortion, which diminishes the reproductive success of the species. The extraction of nuclear DNA from peripheral blood Iymphocytes was performed with GenElute Blood Genomic DNA Kit manufactured by Sigma-Aldrich. DNA isolation from muscles was performed with Sherlock AX set manufactured by A&A Biotechnology. Primers and PCR conditions for the second DQA gene exon were conducted according to (34). Alleles obtained were analyzed for polymorphism by the SSCP method with modifications according to (34). The following primers were used to obtain exon 2 of the DRB gene: DRB2F 5’ - GAG TGT CAT TTC TAC AAC GGG A - 3 `, DRB2R 5’ - CTC CCG AAC CCC GTA GTT GTG TTT GC - 3’. Other reaction conditions were the same as for the DQ gene. Alleles obtained were analyzed with the Arlequin 3.5 program (20). The following parameters were analyzed: expected and observed heterozygocity, the inbreeding level (FIS), and the fixation index (FST). The number of alleles in the population, the number of private alleles, the number of homozygotes and heterozygotes, as well as the allelic richness (R) were determined with the FSTAT v.2.9.3.2 program (23). FIS for the DRB1 locus revealed an HW disequilibrium with a significant excess of DRB1 homozygotes, especially in the Kalisz region. FIS for the DQA 1 locus revealed an HW equilibrium in the Kalisz and Oświęcim regions, whereas in the other regions (Ciechanów and Płock) there was a slight excess of heterozygotes. These results are the first findings for the MHC class II DRB1 and DQA1 loci in a population of Polish hares living in the wild. The above picture of changes in the frequency of genes and DRB1 and DQA1 genotypes shows tendencies towards decreasing heterozygocity and increasing homozygocity. This reveals the local incidence of highly unfavorable phenomena associated with an inadequate gene exchange, resulting in a disturbed HW equilibrium. These findings suggest that poor reproductive performance of Polish hares may be indirectly related to a disturbed HW equilibrium and a significant increase of homozygocity at the MHC class II DRB1 locus.
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