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The objectives of this study were to determine the dominant frequency of plant species, and provide useful information toward the conservation management of animal feed in grazing areas, Hiroshima, Japan. The numbers of plant species were 32 and 21 in spring and summer, respectively. The appearance frequency of plant species more than 50% in the spring, consist of Veronica arvensis (75.57%), Trifolium repens (73.86%), Paspalum dilatatum (69.32%), Lamium purpureum (68.75%), Trifolium dubium (65.34%), Cerastium glomeratum (63.64%), whereas in summer, it consists of Paspalum notatum (98.30%), Trifolium repens (81.25%), Paspalum dilatatum (78.98%), and Kyllinga brevifolia (74.43%). It was observed that the plant high of the plant species were 16.64 and 21.55 cm; vegetation cover rates were 77.18 and 81.36%; chlorophyll content were 41.72 and 36.28 mg/g Fw, and species numbers were 17.91 and 10.18, in spring and summer, respectively. Findings of this research propose that Trifolium repens (clover), Rumex japonicus (weed), and Paspalum dilatatum (grass) are dominant species in the studied areas that can be utilized as animal feeds.
Chloroform, butanolic and water fractions from the methanolic extracts of Lamium album L. and L. purpureum L. flowers and different organs of mistletoe (Viscum album L.), namely leaves, stalks and fruits were investigated for the free radical scavenging properties by using colour free radical DPPH • as a stain reagent for dot-blot assay on a T LC plate and two-dimensional thin-layer chromatography (2D-TLC) analysis (2D-TLC-DPPH test) as well as a dye reagent for the spectrophotometric assay. For each plant material, butanolic fractions showed the strongest activity, of which those of the Lamium species were nearly equal to that of the known antioxidant - BHA. According to 2D-TLC chromatography, the phenolic compounds present were responsible for the antiradical activity of the fractions.
The aim of performed research was to evaluate weed seedbank in soil under the influence of four different winter wheat tillage systems. Winter wheat was grown in the following cultivation systems: A – monoculture with direct drilling into white clover mulch; B – monoculture with direct drilling into wheat stubble; C – monoculture with conventional tillage; D – crop rotation with conventional tillage. It was shown that pre-sowing wheat tillage had a more considerable effect on weed species and weed seedbank in soil than type of crop rotation. The least seedbank was observed when plough system was replaced by direct drilling. In the soil layer of 0–20 cm, under wheat no-plough tillage, 20.3% less weed diaspores wasfound compared to monoculture with plough tillage and by 40.1% lessthan in crop rotation. The plough tillage increased amount of weed diaspores in the whole plough layer, while direct drilling increased it only in 0–1 cm of soil layer. After direct drilling of wheat into stubble (B) the number of weed diaspores in 1 dcm3 of soil in 0–1 cm layer was over twofold higher than in direct sowing in mulch (A), and threefold higher than in crop rotation (D) and almost six times higher than in wheat monoculture with conventional tillage (C). Dominating weed species in the soil over the types of wheat cultivation systems were: Chenopodium album L., Amaranthus retroflexus L., Apera spica-venti L., Lamium purpureum L., and Viola arvensis Murr.
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