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A HPLC method with DAD detection for determination of melatonin in Lamium album flos was developed. The analysis was carried out on RP18 column using mixture of methanol and water (28:72 v/v) as a mobile phase. Established calibration curve (r>0.9994), precision (RDS values ranged from 0.5% to 1.5%), detection limit (0.025 μg/mL) and quantification limit (0.076 μg/mL) were found to be satisfactory for the proposed method. The determined content of melatonin in Lamium album flos was 0.15 μg/g of dry plant material.
The presence of ruderal and mid-field vegetation promotes conservation of biodiversity and provides an additional source of food for insect pollinators. The white deadnettle is a common synanthropic plant visited frequently by various groups of insects, as it is a source of pollen and nectar. In 2012–2013, in the city of Lublin (Poland), signalling and food attractants in L. album flowers were analysed using light and scanning electron microscopy. The pipetting method was used for determination of nectar abundance in the flowers, and the content of sugars in the nectar was assessed with the use of an Abbe refractometer. It was found that the white dead-nettle flowers emitted a fragrance and were equipped with nectar guides, and the corolla and stamens had glandular trichomes and papillae secreting essential oils. On the stamens, there are also non-glandular trichomes, which play a role of pollen presenters. The nectary in the L. album flower has a shape of an irregular disc partly surrounding the base of the ovary. The content of sugars in the nectar was 43%. Sugar and honey yields per ha were calculated, and the values obtained were 153 kg and 191 kg, respectively.
Lamium album (Lamiaceae) is a herb used in therapy of various diseases. Oleanolic and urso­lic acid, bioactive triterpenes which often occur together in Lamiaceae family can influence the biological activity of this plant. A HPLC method with DAD detection was developed for the quantification of these compounds in L. albiflos. The best separation was achieved on RP18 column using mixture of acetonitrile, water and \% phosphoric acid (90:10:0.5 mNN) as a mobile phase, at 0.6 mlVmin flow rate and at a temperature of 10°C. Established calibration curve (r>0.999), precision (RSD values ranged from 0.2 % to 1.2 %), recovery (98.4-101.1 %), detection limit (0.12 /Jg/mL for oleanolic acid, 0.13/Jg/mL for ursolic acid) and quantification limit (0.42 /Jg/mL and 0.43 /Jg/mL, respectively) were found to be satis­factory for the proposed method. The determined contents of oleanolic and ursolic acid in L. albiflos were 33.9 /L/g/g and 112.3 ¿ig/g of dry herb, respectively.
Chloroform, butanolic and water fractions from the methanolic extracts of Lamium album L. and L. purpureum L. flowers and different organs of mistletoe (Viscum album L.), namely leaves, stalks and fruits were investigated for the free radical scavenging properties by using colour free radical DPPH • as a stain reagent for dot-blot assay on a T LC plate and two-dimensional thin-layer chromatography (2D-TLC) analysis (2D-TLC-DPPH test) as well as a dye reagent for the spectrophotometric assay. For each plant material, butanolic fractions showed the strongest activity, of which those of the Lamium species were nearly equal to that of the known antioxidant - BHA. According to 2D-TLC chromatography, the phenolic compounds present were responsible for the antiradical activity of the fractions.
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