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Neustonic (film layer ~ 90 µm, surface layer ~ 240 µm) and planktonic bacteria (subsurface layer ~ 10–15 cm) participating in the processes of decomposition of organic macromolecular compounds, and their potential capability to synthesise extracellular enzymes were studied in a shallow estuarine lake (Lake Gardno – Baltic coast). The studied bacteria were capable of decomposing a wide spectrum of organic macromolecular compounds. Most bacteria inhabiting surface and subsurface water layers hydrolysed lipids, proteins and DNA. The microflora hydrolysing cellulose was represented by the least abundant group of organisms. Of the studied enzymes, alkaline and acid phosphatases, leucine arylaminase, esterase, and esterase lipase were synthesised most actively while β-glucouronidase (βGl) and α-fucosidase (αFu) were synthesized least actively. It can be clearly seen that enzymatic activity was stratified, and there were differences between studied water layers. Bacterial strains isolated from surface and subsurface layers were more active in synthesizing extracellular enzymes than bacteria inhabiting the film layer. Bacteria isolated from various parts of Lake Gardno synthesized the tested hydrolytic enzymes with a similar intensity.
An analysis of the species composition of zooplankton of Lake Gardno was based on the materials collected in the years 1998–2002, from July to August. It was found that the numbers of zooplankton species, their abundance and biomass were greatly variable, and depended on abiotic factors and lake trophy. An analysis of the structural characteristics of zooplankton as bioindicators of lake eutrophication revealed the presence of some species of rotifers and crustaceans in the zooplankton community, considered good indicators of lake trophy. The zooplankton-related trophic state indices enabled to classify Lake Gardno as eutrophic and meso-eutrophic.
This paper presents results of floristic investigations on the southern part of the Gardno Lake. In 2005-2006 vegetation seasons 81 rare, endangered and interesting vascular plants were found. Among them there are 27 species protected by law (19 strictly and 8 partially) (Ustawa… 2004), e.g.: Dactylorhiza majalis, Epipactic helleborine, E. palustris, Galium odoratum, Hepatica nobilis, Lycopodium annotinum, Matteucia struthiopteris, Lilium martagon, Listera ovata, Lonicera periclymenum, Ornithogalum nutans. The Euphorbia palustris found on the investigation area is in direct danger of extinction in the Western Pomerania region.
The aim of this paper was the presentation and comparison of seasonal biogens changes and trophy state classification of two biggest shallow coastal lakes Gardno and Łebsko, located in the northern part of Poland, along the southern coast of the Baltic Sea. Measurements were conducted once a month from April to December 2007. For the purpose of this article at each lake one measurement station was selected, to represent well mixed area of the lakes, far from outlet to the sea and rivers. Performed measurements showed that the lakes are similar. The differences, observed between biogens concentration in lakes were statistically insignificant. Seasonal dynamics of nutrients concentration in lakes indicated that the lakes are typical shallow lakes. Moreover, the summer concentration of total nitrogen and phosphorus qualify these lakes as eutrophic.
The study of heavy metals concentration in the estuarine lake Gardno water and bottom sediments showed that the river Łupawa and periodic inputs of sea water exert decisive influence on forming of the water stores and bottom sediments quality in this lake. The largest concentrations of Cd, Cu, Mn, Pb and Zn were observed at river mouth into the lake region (Cd – 0.26, Cu – 3.71, Mn – 94.3, Pb – 10.01, Zn – 16.26 μg dm-3) and the lowest at water outflow from the lake region (Cd – 0.13, Cu – 1.81, Mn – 53.8, Pb – 8.86, Zn – 8.05 μg dm-3). Average concentrations of those metals in the lake water, suspension and bottom sediment form the following row: Mn>Zn>Pb>Cu>Cd. The contents of analysed metals were the highest in interstitial water, smaller in near-bottom water and the smallest in surface water. Average content of metals in near-bottom suspension was about 30% higher than that in surface suspension, but enrichment factors of those metals in suspensions do not show essential statistic differences. This probably results from continual mixing of water masses by wind. The migration coefficients indicated that bottom sediments of lake Gardno have large accumulation properties of heavy metals.
The abundance of the spawning population of roach females from the coastal lakes Gardno (2 468 ha) and Łebsko (7 140 ha) located in the Słowiński National Park was estimated for the 1987-1992 period. Following bream, roach is the second most common cyprinid species in regulated fisheries catches. The size of the spawning population was estimated using Virtual Population Analysis and long-term fisheries statistics. Female roach dominated the exploited population at 62.4% in Lake Gardno and 64.3% in Lake Łebsko. During the analyzed period, more roach females were caught in Lake Gardno, which is more than twice as small as Lake Łebsko. Of the generations analyzed (1985, 1986, 1987, 1988, 1989 and 1990), more abundant years occurred in Lake Gardno. The most abundant generation in the two lakes was that of 1985, and following it, each subsequent generation was “weaker” than the preceding had been.
Antibiotic resistance of heterotrophic bacteria isolated from the surface and subsurface water of estuarine lake Gardno was determined. The levels of resistance of bacteria to various antibiotics differed considerably. Antibiotic resistance between neustonic and planktonic bacteria and microflora inhabiting different parts of lake Gardno was nearly identical. Besides gentamycin, no differences in the antibiotic resistance between pigmented and non-pigmented bacteria were noted. Majorities of bacterial strains were characterised by resistance to 4-6 antibiotics. Bacterial resistance to antibiotics was dependent on chemical structure.
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Sea water intrusions to the Lake Gardno

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Lake Gardno, as the central object in the hydrographic structure, is under the influence of land and sea waters. This results in the situation where the lake together with its direct catchment forms a unique geoecosystem which differs from other inland reservoirs performing the role of local or regional recipients in hydrographic systems, in terms of the quality of water as well as the rate and dynamics of transformations occurring in it. This is an effect of overlapping influences of waters inflowing from the catchment and intrusions of sea waters. During intrusions, waters of higher salinity occur and remain during the greater part of the year in the whole water body of the lake, which is conditioned by the shape of the basin facilitating the penetration of salty waters. It was established that the water coming from intrusions remains for at least several days. There is also evidence from reconnaissance measurements that the retention is longer. The easiness of penetration and long retention period result in the fact that only periodically the desaltation effect of potamic waters leads to an almost complete desaltation of water in the whole basin of the lake Gardno.
Ciliates constitute an important trophic link in aquatic environments, because they are essential food sources for zooplankton and fish. In this study, the spatial differences in abundance and composition of ciliate communities in relation to varying water salinity were investigated. The study was conducted in the large, brackish, eutrophic lakes Gardno and Łebsko. In the studied lakes, no significant differences among ciliate communities were identified among sampling sites. Ciliate communities in Lake Łebsko were characterized by a high contribution of tintinnids, with mean values at particular sites between 32% and 36%. In Lake Gardno, where salinity was lower, the importance of tintinnid ciliates increased after a marine water intrusion into the lake. In both lakes, the importance of tintinnids in ciliate communities was higher than in adjacent fresh and marine waters. Shortly after wind-driven intrusions of marine waters (backwaters), the marine and brackish mixotrophic ciliate Mesodinium rubrum was observed in both lakes. Once, in the eastern part of Lake Łebsko, Mesodinium major (= Mesodinium rubrum forma major) was observed after a strong backwater when salinity reached 5.4 PSU.
Results of the studies on the occurrence of bacteria displaying particular physiological properties in coastal Lake Gardno are presented. Most numerous groups among neustonic and planktonic bacteria studied were ammonifying and producing hydrogen sulphide from organic compounds strains. Nitrifying bacteria were not numerous among isolated strains. It was demonstrated that significant differences in the abundance of individual physiological groups bacteria existed in water layers, stations and seasons.
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