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Phylogenetic analysis based on 16S rRNA gene of a thermophilic protease-secreting Bacillus gelatini-TPNK-3 isolate from Kiteezi Landfill, Uganda

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Omara J., Ekeya T. P., Hawumba J. F.

Polish Journal of Microbiology

2012

tom 61

nr 3

A thermophilic protease-secreting bacterial isolate, TPNK-3, from Kiteezi landfill, is an aerobic Gram-positive spore-forming bacterium with rod-shaped cells (3.28 μm long and 0.45 μm wide). Optimal growth was observed at 55°C and pH of 7.0, and the isolate tolerates up to 5% (w/v) NaCl, exhibits extracellular amylolytic, cellulolytic and caseinolytic activities, utilizes a range of carbon and nitrogen sources and has a GC content of 45 mol%. The 16S rRNA gene sequence analysis showed that the studied bacterium belongs to the genus Bacillus, and closest to Bacillus gelatini (> 99.9%). Consequently, isolate TPNK3 is tentatively described as Bacillus gelatini strain TPNK3.

Ograniczanie wyników

1 Polish Journal of Microbiology

1 Ekeya T. P.

1 Hawumba J. F.

1 Omara J.

1 2012

Wyniki wyszukiwania

Phylogenetic analysis based on 16S rRNA gene of a thermophilic protease-secreting Bacillus gelatini-TPNK-3 isolate from Kiteezi Landfill, Uganda