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A total of 26 isolates of Fusarium proliferatum and F. sacchari were isolated from rice in the Peninsular Malaysia and Kalimantan, Indonesia. Spontaneous chlorate-resistant sectors (CRSs) were recovered from all wild type of both Fusarium species when cultured on two chlorate media. The non-utilizing (nit) mutants were generated as crn (chlorate resistant, nitrate utilizing), nit1, nit3 and nitM based on phenotyping growth-types on diagnostic media with different sources of nitrogen. The nit mutants were paired on minimal medium (MM) for examining the vegetative compatibility. The majority of nit mutants (32.3-46.5%) recovered were nit1. Eight and seven vegetative compatibility groups (VCGs) of F. proliferatum and F. sacchari were identified, respectively. The isolates of F. proliferatum and F. sacchari were genetically diverse as shown by the number and distribution of the VCGs. No strong correlation was observed between VCGs of both species and location.
Borneo has a significant role to play in the conservation of bat diversity in Southeast Asia, yet there is little taxonomic and distribution information available for the Indonesian states that represent over two thirds of the island. We report the results of a four week harp-trap and mistnet survey of four limestone karst formations on the Sangkulirang peninsula in East Kalimantan during August 2004. We recorded 36 taxa, including two (a Pipistrellus and Rhinolophus) that may represent new species, and three (Hipposideros bicolor, Myotis horsfieldii and M. montivagus) that are new records for Kalimantan. Several species, including the karst dependent and patchily distributed Hipposideros larvatus, Rhinolophus creaghi and R. pusillus, were highly abundant at the formations. In Borneo, many of the species were previously known from only a few, or scattered, localities including four Red-Listed species (R. creaghi, M. montivagus, Murina rozendaali and Kerivoula minuta). We review the Bornean distributions of individual species given recent surveys in Kalimantan and present taxonomic data from 135 specimens collected during this study. This represents the largest bat collection in Kalimantan so far undertaken and highlights the Sangkulirang peninsula as a key site for bat diversity and conservation in Borneo. Threats to this area include disturbance from bird nest collection in caves, but also mass disturbance from logging and forest fires. We advocate inclusion of the formations and associated forests into a protected area to safeguard this biological resource.
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