The inhibitory effect of crab-shell chitosan. medium (200-800 cps) and high molecular weight (800-2000 cps) (purchased from Sigma-Aldrich Chemicals) toward Alternaria alternata , Botrytis tulipae, Fusarium oxysporum f. sp. callistephi, Fusarium oxysporllm f. sp. tulipae, Phoma narcissi and Phoma poolensis was evaluated in vitro and in vivo. The chitosan evidently inhibited in vitro growth of all tested pathogens, with a marked effect at higher concentrations above 200 Ilg/cm3. Chitosan at a concentration 01' 1.25; 2.5 and 5.0 mg/cnY didn't have inhibitory action in appearance of fungi growth on naturally contaminated Callistephlls chinensis seeds. At the same concentrations, chitosan applied as bulb scales dressing of Hymenocallis narciss (flora bulbs, before inoculation or after inoculation with Phoma narcissi, inhibited the development of necrotic spots on scales. Chitosan used preventively or curatively at a concentrations of 1.25; 2.5 and 5.0 mg/cm3 indicated inhibitory effect on development of Fusarium oxysporum f. sp. Tulipae on tulip bulbs. Chi to san at a concentration of 10 mg/cm3 applied preventively (first spray 12th June) was very effective in the control of Puccinia antirrhini on snapdragon in the field. The strongest inhibitory effect was observed on snapdragon treated 8 times at week intervals.
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