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The aim of this study was to investigate the effects of three aging processes (traditional dry aging, wet aging in vacuum shrink pack and dry aging in a highly moisture-permeable bag) in five aging times (0, 7, 14, 21, 28 days) for their effects on physical, chemical, microbial, histological, textural and sensory properties of beef Longissimus lumborum muscle from Holstein-Friesian steers. Longissimus lumborum muscles were aged dry (D), in a dry-aging bag (B) and a vacuum shrink pack (V) for 28 days at 0.5°C±0.5 with 80% humidity. An increase was observed in pH and TBARS values of all samples. All methods caused a decrease in the WBSF value. The decrease of WBSF was rapid until the 14th day. V samples had lower WBSF values in comparison with others. While the L*and a* values were similar on the 28th day in all groups, b* values were different (p<0.05). There were no significant effects of aging treatment on the sarcomere length on the 28th day (p>0.05). An increase was also observed in the numbers of the total viable count, total psychrophilic bacteria, Enterobacteriaceae, lactic acid bacteria and yeast-mold in all groups. B aging may have a positive effect on safety, quality, yield and shelf stability of aged-beef. V aging significantly improves both textural and sensory characteristics of meat. The most positive effect on flavor, juiciness, tenderness and overall like were observed in the V aged treatment on the 14th day of aging. The use of the vacuum shrink pack is considered to be an alternative packing material in the wet aging method to improve the physicochemical and sensory quality of beef.
The aim of this study was to verify the hypothesis that insertion/deletion (indel) polymorphisms within the promoter and intron 1 sequences of the prion protein (PRNP) gene can affect the breeding value of Holstein-Friesian bulls. The experimental material included 261 Holstein-Friesian bulls born between 1997 – 2002. It was shown that the polymorphism in the intron 1 (12 bp indel) sequence had a more significant effect on the analyzed traits than a polymorphism in the promotor (23 bp indel) sequence. A deletion allele within intron 1 (12del) significantly increased the bulls’ breeding value for milk yield (p=0.001), protein yield (p=0.042), type and conformation (p=0.018),udder width (p=0.003), dairy character (p=0.004), and decreased days open (p=0.022). A deletion allele (23del) at the polymorphic locus of the promoter significantly decreased the bulls’ breeding value for milk yield (p=0.001), udder width (p=0.029) and dairy character (p=0.033). Analysis of both alleles showed that 23del-12del haplotype increased the bulls’ breeding value for udder traits:udder (p=0.008), fore udder (p=0.043) and udder depth (p=0.041), and decreased for fat kontent (p=0.047) and conformation traits - body depth (p<0.001), chest width (p=0.029) and rear leg set - side view (p=0.029). Also positive effect of 23del-12ins haplotype for days open (p=0.003) and days between calving and first insemination (p=0.045) was observed.
Relationship between the parity and oxidative stress in high-performance Polish Holstein-Friesian cows after the peak of lactation. There are many factors, which may expose cows attacks of free radicals. The highest level of oxidative stress appears in the parturition period and at the peak of lactation. Therefore, the purpose of this study was to demonstrate the relationship between the parity and oxidative stress in high-performance Polish Holstein-Friesian (PHF) cows after the peak of lactation. Seventy PHF cows were selected for the experimentaccording to: age (35 primiparous and 35 multiparous in the second lactation) and stage of lactation (after the peak of lactation; at 61-90days in milk). Samples of milk and blood were collected in monthly intervals, from 61-90till >271 day of lactation. Study results demonstrated a significant impact of the parity and days in milk of cows on the formation of oxidative stress markers. The primiparous cows were characterized by lower levels of Glu Red and Gpx. The lowest level of oxidative stress was observed in the months after the peak to about 250 days of lactation. Based on study results, it can be concluded that that younger animals were more exposed to free radicals and oxidative stress.
The aim of this study was to determine the effect of the origin of Holstein-Friesian cows on the yield, concentration of protein fraction components and selected technological parameters of cow’s milk. The fifty Holstein-Friesian cows from Poland, Sweden and Germany were involved in the experiment. The analysis of milk, protein and fat yield showed advantage of cows from Poland over those imported from Sweden and Germany. On the other hand, the most advantageous protein/fat ratio was found in the milk of Swedish cows. The analysis of the selected technological parameters of milk including the country of origin of cows revealed that the milk from Polish cows was characterized by a higher content of casein, dry matter and solids-not-fat than imported cows. The highest concentration of β-lactoglobulin and bovine albumin serum was observed in the milk of Swedish cows, whereas the milk from Polish cows was characterized by the highest level of α-lactoalbumin, lactoferrin and lactoperoxidase. The results of this study show that only the introduction of animals with high genetic potential can bring financial benefits expected from the sale of raw milk. Origin of animals plays an important role in determining of whey protein fraction and technological usefulness of milk.
Similarly to the production of red blood cells, the production and development of leukocytes takes place in the bone marrow in the process of hematopoiesis. The key stages in the development of leukocytic cell lines are: neutrophilic, eosinophilic and basophilic granulopoiesis, monocytopoiesis, lymphopoiesis and megakaryopoiesis. Leukocytes produced at various developmental stages are characterized by differently shaped nuclei, the presence or absence of nucleoli, and variations in cytoplasm staining. The experiment was carried out on 10 clinically healthy HF cows. Bone marrow aspirate smears stained by the MGG method were analyzed. The study found significant differences in the number, size, and staining intensity of individual myeloid cell lines.
The aim of this study was to detect heifers with dystocia using artificial neural networks (ANN). A total of 531 calving records of Holstein-Friesian heifers of Black-and-White strain and 8 diagnostic variables were used. The output variable was the class of calving difficulty: difficult or easy. Perceptrons with one (MLP1) and two (MLP2) hidden layers and radial basis function (RBF) networks were investigated. The root mean square error and the structure of selected ANN (number of neurons in the input, hidden and output layers) were 0.22, 10-4-1; 0.25, 10-17-17-1 and 0.19, 10-25-1 for MLP1, MLP2 and RBF, respectively. The percentage of correctly recognized heifers with difficult and easy calvings and that of correctly diagnosed heifers from both categories for the training and validation sets were approx. 90%. The same values for the test set were 75-83%, 82–88% and 82–86%, respectively. In both cases, no significant differences in these proportions were found. The following variables contributed most to the detection of heifers with dystocia: gestation length, BCS index, CYP19-PvuII and ERα-BglI genotypes and percentage of HF genes in heifer’s genotype.
The aim of this study was to create new strategies to increase the pregnancy rate, in "ovsynch protocol" treatment. Two programs for synchronisation of ovulation and for synchronisation of ovulation and oestrus, similar to the ovsynch, were developed for the use in lactating primiparous dairy cows. Lactating Holstein-Friesian cows were randomly divided into five treatment groups: the GPG group (ovsynch) was treated with GnRH on day 0, PGF (PGF2α) on day 7, and received the second dose of GnRH 48 h later; the groups -7PGPG and - 2PGPG received the same treatment as the GPG group, but were given an additional injection of PGF 7 and 2 d before the start of the GPG treatment; respectively, the PG9PG group received the same treatment as the -2PGPG group, with the modification that the first GnRH injection was given simultaneously with the first PGF on the 2nd d; the GPEG group received the same treatment as the GPG group, but was injected an additional oestradiol propionate (EP) 24 h after the PGF. Plasma progesterone concentrations were determined at the days of the first hormone injection and the last PGF injection. Ovulation rates after the first GnRH and last PGF injections were calculated and presumptive sizes of the follicles on the last PGF injection day were determined in all the cows by rectal palpation. Cows detected to be at oestrus in 72 h after the last PGF injection was inseminated between the 8th and 12th h of their oestrus. Cows not detected to be in oestrus by 72 h after the last PGF received timed artificial insemination (TAI). While the ovulations mostly occurred in the GPG, GPEG, and -7PGPG groups at a period between the 48th and 96th h after the last PGF injection, the ovulations had shifted and occurred between 72 and 120 h, with 66.7% of all ovulations recorded between 72 and 96 h in the -2PGPG group. In the PG9PG group, ovulations took place dispersedly between the 0th and 120th h after the last PGF injection. The pre-synchronisation treatment (-7PGPG) by a PGF injection 7 d prior to the ovsynch protocol did not enhance the ovulation or pregnancy rates. The pre-synchronisation treatment by PGF injected 2 d before the ovsynch protocol, increased the number of cows that ovulated after the first GnRH injection (88.9% vs. 38.9%; P<0.05), produced smaller follicles at the time of the second PGF injection (1.1±0.13 vs. 1.6±0.16 cm; P<0.05) and increased pregnancy rates numerically (72.2% vs. 50.0%; P>0.05). Adding EP to GPG (GPEG), enhanced the expression of oestrus (P<0.05) and increased pregnancy rates slightly (60.0% vs. 50.0%; P>0.05). In conclusion, the -2PGPG and GPEG treatments are potentially new methods for routine synchronisation of ovulation and oestrus and/or ovulation, respectively, in primiparous Holstein-Friesian cows.
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