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Characterization of the phenylalanine ammonia lyase gene from the rubber tree (Hevea brasiliensis Müll. Arg.) and differential response during Rigidoporus microporus infection

100%

Sangsil P., Nualsri C., Woraathasin N., Nakkanong K.

Journal of Plant Protection Research

2016

tom 56

nr 4

Phenylalanine ammonia lyase (PAL) is a specific branch point enzyme of primary and secondary metabolism. It plays a key role in plant development and defense mechanisms. Phenylalanine ammonia lyase from Hevea brasiliensis (HbPAL) presented a complete open reading frame (ORF) of 2,145 bp with 721 encoded amino acids. The sequence alignment indicated that the amino acid sequence of HbPAL shared a high identity with PAL genes found in other plants. Phylogenetic tree analysis indicated that HbPAL was more closely related to PALs in Manihot esculenta and Jatropha curcas than to those from other plants. Transcription pattern analysis indicated that HbPAL was constitutively expressed in all tissues examined, most highly in young leaves. The HbPAL gene was evaluated by quantitative real-time PCR (qRT-PCR) after infection with Rigidoporus microporus at 0, 12, 24, 48, 72 and 96 hours post inoculation. The expression patterns of the PAL gene differed among the three rubber clones used in the study. The transcription level of the white root rot disease tolerant clone, PB5/51 increased sharply during the latter stages of infection, while it was relatively subdued in the white root rot disease susceptible clones, RRIM600 and BPM24. These results suggest that the HbPAL gene may play a role in the molecular defense response of H. brasiliensis to pathogen attack and could be used as a selection criterion for disease tolerance.

The application of topographical survey and the arcgis spatial analys “Darcy Flow” tool in managing the nurseries of rubber tress

100%

Eboigbe M. A., Obasogie F. O., Alasah I., Idiata D. J.

International Letters of Natural Sciences

2014

tom 13

nr 2

Farmers often rely on the physical appearance of rubber tree seedlings to decipher on growth rate. In situations of doubt especially when there is obvious reduction in chlorophyll or coax decay of roots, the most likely situation is induction by flooding. A sound nursery management technique which is both responsive to continuous monitoring of the terrain and also able to generate data for restoration is most desired. This study therefore employs a geospatial technique whereby topographical survey was used to pick field data while Geographical Information System analyses was able to generate accurate information on areas likely to both surface and underground discharge of materials. This method was also able to sum up all affected areas, alongside other attributes like the direction of surface flow and the rate of discharge.

Ograniczanie wyników

1 International Letters of Natural Sciences

1 Journal of Plant Protection Research

1 Alasah I.

1 Eboigbe M. A.

1 Idiata D. J.

1 Nakkanong K.

1 Nualsri C.

1 Obasogie F. O.

1 Sangsil P.

1 Woraathasin N.

1 2016

1 2014

Wyniki wyszukiwania

Characterization of the phenylalanine ammonia lyase gene from the rubber tree (Hevea brasiliensis Müll. Arg.) and differential response during Rigidoporus microporus infection

The application of topographical survey and the arcgis spatial analys “Darcy Flow” tool in managing the nurseries of rubber tress