The procedure of in vitro propagation of Harpagophytum procumbens using shoot tips was developed. Shoot tips were cultured on Schenk and Hildebrandt (SH) agar medium supplemented with 0.57 µM indole-3-acetic acid (IAA) in combination with various cytokinins: 6-benzylaminopurine (BAP), thidiazurone (TDZ), kinetin or zeatin at four concentrations (2, 4, 6 or 8 µM). The best shoot multiplication rate (11.2 shoots/explant for 5 weeks) was achieved in the presence of 6 µM TDZ. The shoots were small and their elongation on SH medium supplemented with gibberellic acid (GA3 ) was necessary. Shoots of H. procumbens were rooted on full-strength or half-strength Murashige and Skoog (MS) agar medium either with or without auxin. Plantlets were transferred into pots and maintained in the greenhouse. After 1 month, the overall survival of plants was 83% but it decreased to 27% after 6 months.