The study was conducted in the Pomological Orchard and a greenhouse complex of the Research Institute of Horticulture in Skierniewice (RIH) in 2006-2008. Its aim was to identify arbuscular mycorrhizal fungi (AMF) present in the trap cultures containing rhizosphere soil and to determine mycorrhizal frequency (colonization) of AMF in the roots of apple trees 'Gold Milenium' and blackcurrant bushes 'Tiben'. The apple trees and blackcurrant bushes were mulched (with a peat substrate, bark, sawdust, manure, compost, or straw) and inoculated (the AMF inoculum was produced by MYKOFLOR). Samples of the soil and roots were collected from under the apple trees and blackcurrant bushes in experimental combinations and in the control. In order to identify the spores of arbuscular mycorrhizal fungi, trap cultures (with rhizosphere soil and sand) were set up with plantain (Plantago lanceolata L.). The spores were isolated from the trap cultures and microscopic specimens were prepared to identify the species of AMF, which were distinguished on the basis of their morphological features. Mycorrhizal frequency was determined in the specimens of apple and blackcurrant roots dyed with aniline blue. Vol. 19(1)2011: 35-49 In total, eight species of AMF were identified in the trap cultures established with the soil samples taken from the root zone (containing rhizosphere soil) of apple trees 'Gold Milenium' and blackcurrant bushes 'Tiben': Glomus aggregatum, G. caledonium, G. claroideum G. constrictum, G. intraradices, G. macrocarpum, G. mosseae and Gigaspora margarita. In the trap cultures with the rhizosphere soil ofapple, the greatest number of species was found in the combinations with manure and the mycorrhizal inoculum (5 species), and compost (4 species). In the case of blackcurrant, the use of the mycorrhizal substrate and straw resulted in the largest number of AMF species (5 and 4, respectively). In the 2008 season, the highest mycorrhizal frequency in apple was obtained in the mycorrhized roots (44.4%), lower in the combinations with compost, sawdust, manure, bark, straw, and peat, and the lowest in the roots of NPK control plants (5.56%). The highest mycorrhizal frequency in the roots of blackcurrant bushes cv. 'Tiben' was recorded following the application of the mycorrhizal inoculum (12.22%), lower in the combinations with sawdust, compost, straw, peat, manure, and bark, and the lowest in the NPK control (1.67%).
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