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Plant growth and physiological response of sesame (Sesamum indicum L.) were studied in controlled environment using normal soil and indigenous Vesicular-arbuscular mycorrhiza (VAM) fungi treated soil. The seedlings of Zea mays were inoculated with Giguspora species of VAM (Glomus fasiculatum) and the inoculum was multiplied with help of Zeamays seed bed. Sesame seeds were then inoculated into the bed and it was found that the plant height, shoots lengths, roots, biomass of shoot and roots were considerably increased in the mycorrhizal plants. The effect of VAM infection was assessed in pot experiment. In this comparative study, specific mycorrhizal fungi had consistent effects on various growth parameters such as the number of leaves, number of roots, shoot length, biomass of shoot and roots and biochemical parameters were observed at various time intervals by statistical analysis using two way ANOVA, it was confined with mycorrhizal and non-mycorrhizal infected plants. It was found that the ability of isolates to maintain the plant growth effectively in the case of mycorrhizal seedlings shows a maximum absorbtion of 0.77 ±0.2, shoot length is about 8.34 ±0.2, count of root and leaves are about 8.10 ±0.3, 5.6 ±0.3 respectively under mycorrhizal infection in 30days of analysis and had a positive effect on the growth at all intervals. Biochemical analysis were carried out to estimate the total chlorophyll, chrophyll A, chlorophyll B and Carotenoids contents and it was analyzed to be 9 ±0.5 mg/g, 8.3 ±0.5 mg/g, 3.6 ±0.5 mg/g, 4 ±0.3 mg/g respectively. At the 30th day of analysis for the mycorrhizal plants, it was found to be high in mycorrhizal seedlings which shows the symbiosis had improved the nutrient uptake of cultivated plants. Nevertheless G. fasiculatum was found to be the most efficient fungus and exhibited the highest levels of mycorrhizal colonization, as well as the greatest stimulation of physiological parameters.
Fusarium oxysporum and Rhizoctonia solani are the major soil-borne pathogens causing growth and yield depression. The present study focused on the ability of fluorescent Pseudomonas and Glomus fasciculatum on growth performance of Vigna radiata in pathogen-infested soil. The percent colonization by G. fasciculatum indicated an increase of the presence of fluorescent Pseudomonas and a decrease of the presence of Fusarium oxysporum or Rhizoctonia solani. However, the reduction of colonization induced pathogen in percent was alleviated by fluorescent Pseudomonas. Inoculation with either fluorescent Pseudomonas or G. fasciculatum or both induced a significant increase in root and shoot length, plant vigour index, dry weight and total N and P content in V. radiata as compared to uninoculated control. The impact of inoculation was much pronounced in dual inoculated plants in comparison with those inoculated with either G. fasciculatum or fluorescent Pseudomonas. In contrast, treatment of plants with either F. oxysporum or R. solani decreased the root and shoot length, plant vigour index, dry weight and total N and P content in the test legume. However, in the presence of fluorescent Pseudomonas and G. fasciculatum, the adverse effect on the pathogens on growth of V. radiata was alleviated.
Biofertilizers have been used as sources to improve plant nutrients in sustainable agriculture. Biofertilizers are low cost, renewable sources of plant nutrients which supplement chemical fertilizers. Mycorrhizal fungi are a major component of agricultural natural resources and members of kingdom fungi. The term ‘mycorrhiza’ is derived from Greek word which means ‘fungus root’. Earthen pot experiments were carried out to study the inoculation effect of Arbuscular Mycorrhizal (AM) fungi (Glomus mossease), Azospirillium brasilense and PSB on plant height, dry weight of root and shoot, per cent root colonization, spore number, P and N uptake. Experimental pots were filled with 4 kegs of sterilized soil and maintained in green house at 25-30 °C temperature. Single inoculation of AM fungi and combined inoculation of AM fungi with Azospirillium brasilense or PSB was found to be moderately increased in all the growth parameters. However triple inoculation of AM fungi, Azospirillium brasilense and PSB was found to have highest growth parameters.
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