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The pathogenicity of Fusarium avenaceum, F. culmorum and F. equiseti to thyme was examined in present work. Isolates of tested fungi originated from various herbs plants. The effect of Fusarium spp. post-culture liquids and water suspensions of Fusarium spp. spores on germination of thyme schizocarps was studied in the laboratory. The effect of Fusarium spp. on the shooting up and healthiness of seedlings was carried out in the climatic chamber conditions using the method with infested soil. It was shown, that harmfulness of F. avenaceum, F. culmorum and F. equiseti to thyme, regardless of the origins of fungi isolates, was expressed by inhibition of schizocarps germination and by limiting of seedlings numbers and their growth. The symptoms of infection were a necrosis of shoots and roots. Each of the three used methods of artificial infection of thyme secured direct contact of with the surface of schizocarps, shoots and roots of seedlings. However the method with Fusarium spp. post-culture liquids should be recommended to the fast estimation of Fusarium spp. pathogenicity to thyme on account of occurrence of toxins, which are produced by fungi.
The aim of the present work was to determine the pathogenicity of Fusarium avenaceum, F. culmorum, F. equiseti isolates, obtained from various species of herbs, to lemon balm. Three different tests of pathogenicity were carried out. The effects of Fusarium spp. post-culture liquids and water suspension of conidia on germination of lemon balm schizocarps were studied in laboratory conditions. The effect of Fusarium spp. on shooting up and healthiness of seedlings was carried out in climatic chamber conditions using the method with infested soil. All the isolates of fungi species studied caused necrosis of shoots and roots, regardless of their origins. From among three methods of artificial infection used, the method with Fusarium spp. post-culture liquids secured a fast estimation of pathogenicity the fungi isolates to lemon balm. The studied isolates of Fusarium spp. were recognized as pathogenic to lemon balm on the base of positive results in pathogenicity tests and on the base of their earlier isolation from different organs: schizocarps, roots and the bases of stems, taken from the plantations of lemon balm.
One of the reasons lowering the esthetic appearance of a lawn are diseases caused by fungi. The study on seed material of 11 ornamental grass species was carried out in 2009 and 2010. 681 fungi isolates belonging to 22 species and non-sporulating forms were obtained over the two year study period. Among fungi pathogenic to grasses, species of the genus Fusarium were obtained especially in 2010. In that year, isolates of these fungi constituted 29.94% of all isolated colonies. Colonies of Alternaria alternata were also frequently isolated from the analyzed seed material of the ornamental grasses; in 2009 and 2010 its isolates accounted for respectively 25.36 and 12.57% of all isolates. The study on the health of ornamental grasses conducted in field conditions revealed the occurrence of plants with necrosis of the roots and lower stem internodes. The percentage of such plants ranged from 12% for Coix lacryma-jobi to 69% in the case of Festuca ovina. The mean values of the diseases indices were from 2.6 in the case of Coix lacrymajobi to 39.6 for Lagurus ovatus and they differed significantly. From the infected plants mainly species of genus Fusarium were obtained. Majority of isolates consisted F. oxysporum and also F. culmorum, F. crookwellense, F. graminearum, F. solani, F. avenaceum, F. sporotrichioides and species Bipolaris sorokiniana and Exserohilum pedicellatum were obtained. Studies on susceptibility of the seedlings of seven ornamental grass species to infection by Fusarium culmorum No. 34 and F. equiseti No. 62 were conducted in a growth chamber. The statistical analysis of disease indexes for plants that grew in the experimental combination with artificial infection of the subsoil with abovementioned strains as compared to the control indicated significant differences in all studied species in the case of F. culmorum and in 5 species in the experimental combination with F. equiseti. Strain Fusarium culmorum No. 34 proved to be the most pathogenic towards Festuca glauca (98.25), and F. equiseti No. 62 towards Phalaris canariensis (89.5).
Fusarium sp. has been shown to be a promising organism for enhanced production of xylanases. In the present study, xylanase production by 21 Fusarium sp. isolates (8 Fusarium culmorum, 4 Fusarium solani, 6 Fusarium verticillioides and 3 Fusarium equiseti) was evaluated under solid state fermentation (SSF). The fungal isolate Fusarium solani SYRN7 was the best xylanase producer among the tested isolates. The effects of some agriculture wastes (like wheat straw, wheat bran, beet pulp and cotton seed cake) and incubation period on xylanase production by F.solani were optimized. High xylanase production (1465.8 U/g) was observed in wheat bran after 96 h of incubation. Optimum pH and temperature for xylanase activity were found to be 5 and 50°C, respectively.
The occurrence and harmfulness of fungi towards Pastinaca sativa L. parsnip of White Gem cultivar were determined in 2005–2007. The mycological analysis of schizocarps with spots and without spots, seedlings and plants at the time of harvest was conducted every year. The fungi were identified on the basis of etiological symptoms visible on the infected parts of plants and on the basis of the results of mycological analysis. Parsnip schizocarps were colonized by various fungi species. Larger frequency of occurrence and biodiversity of fungi species on plants at the time of the harvest was found as compared to the seedlings. The fungus Itersonilia pastinacae, recognized as a cause of black cancer of parsnip in the regions where this plant is cultivated, was isolated for the first time in Poland. Alternaria alternata, A. raphani, Fusarium oxysporum, F. equiseti, F. solani and Stemphylium botryosum belonged to the species of fungi frequently isolated from various parts of this plant.
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