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For breeding purposes, the number of neo-tetraploids of apple cultivars have been derived by in vitro technique. The first attempts at rooting and acclimatization of tetraploid shoots failed. The aim of the study was to develop an effective method for rooting microcuttings of apple neo-tetraploids. In the first stage of the study, in vitro rooting method was optimized for the shoots of diploid donor cultivars. Shoots were rooted on Murashige and Skoog [1962] (MS) medium with a reduced content of nitrogen, in the presence of auxins alone or in combination (indole-3-butyric acid – IBA, 1-naphthaleneacetic acid – NAA and indole- -3-acetic acid – IAA) with addition of putrescine, arginine or ornithine. The compounds were applied continuously for 25 days (one-step rooting system) or for seven days with subsequent transplanting shoots onto a medium without these compounds (two-step rooting method). Tetraploid microcuttings of the cultivars ‘Free Redstar’, ‘Gala Must’, ‘Pinova’ and ‘Redchief’ were evaluated for rooting on the selected medium considered optimal for their diploid counterparts. The shoots of all diploid apple scion cultivars had low rooting capacity. IBA alone poorly stimulated root formation. Significant improvement of rooting to 60–80% was achieved through the application of auxins, 2.5 µM IBA or 1.3 µM NAA combined with 5 µM IAA and 50 µM putrescine in the two-step rooting system with darkness and increased temperature of 26°C during seven-day induction phase. The replacement of benzyladenine (BA) by meta-Topolin (m-T) in the last multiplication subculture influenced positively shoot acclimatization. Tetraploids had comparable or slightly lower rooting and acclimatization ability compared to their diploid counterparts.
Suitability of nine scab-resistant or partially scab-resistant apple cultivars: Bohe­mia, Rubinola, Topaz, Goldstar, Pinova, Redkroft, Free Redstar, Melfree and Gold Milenium for organic orchards was evaluated during the period 2002-2010. The trial consisted of two experimental plots. In the first plot, no plant protection chemicals were applied either to the trees or to the soil. In the second plot, with limited spraying programme, only those compounds that are allowed to be used in organic fruit pro­duction in Poland were applied in order to control pests and diseases: copper prod­ucts, Carpovirusine Super SC and the garlic extract Liquid Bioczos. After planting, the ground in the tree rows was mulched with agricultural spun-web, linen residue felt or sawdust in a 1.2 meter wide strip. During the years of the experiment many trees were seriously infected with apple canker (Nectria sp.), which caused the loss of a certain number of them. Most susceptible were 'Rubinola', 'Topaz', 'Redkroft' and 'Pinova'. The smallest necrotic lesions were observed on the trees of 'Free Redstar', 'Melfree' and 'Gold Milenium'. The various materials for mulching did not signifi­cantly affect the size of the fruit yield. The highest yields were obtained from the 'Gold Milenium', 'Free Redstar', 'Topaz' and 'Pinova'. In 2010, following a very rainy season, we observed for the first time scab potted fruits of the cultivars: Rubino- la, Topaz, and Goldstar. The greatest problem was the control of aphids and apple mildew. Cutting back shoots with primary infestation was the most promising me­thod. The 'Topaz', 'Rubinola', 'Bohemia' and 'Gold Milenium' appeared to be the most suitable for organic fruit production. In the year 2010, heavy thinning of fruitlets helped to obtain large fruits (diameter over 7.0 cm) of the cultivars: Bohemia, Topaz and Redkroft.
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