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Gene pool variation of twenty varieties and breeding clones of Fragaria × ananassa, nine varieties and breeding lines of Fragaria vesca, and one new interspecific hybrid designated Fragaria × anavesca was analysed with three DNA marker systems. ISSR reactions with four primers produced 45 polymorphic markers. Similarly, RAPD analyses with three primers produced 26 markers and SSR method with three primer pairs revealed 28 different alleles. The total number of 99 polymorphic markers allowed distinguishing clearly a group of F. × ananassa genotypes from that of F. vesca genotypes with F. × anavesca in between of these two. RAPD markers proved to be more informative than ISSRs as 3 of 26 were specific to F. × ananassa only and one exclusively to F. vesca and F. × anavesca. Thus, the presumed hybrid nature of F. × anavesca was effectively confirmed by RAPD markers. Especially important was the 1100bp long PCR product of the B104 primer present in all F. vesca genotypes as well as in F. × anavesca but absent in F. × ananassa. Presence of F. vesca DNA in the hybrid F. × anavesca was additionally corroborated by the 223bp product of the UDF017 primer pair and the 185bp-long band generated with the UDF006 primer pair.
In the paper a classification of 117 genotypes (cultivars and clones) in a strawberry collection from the Research Institute of Pomology and Floriculture in Skierniewice was assessed. The classification of genotypes was based on nine characters including some vegetative attributes as well as those describing fruit yielding and susceptibility to the most important strawberry diseases. Data came from a trial observed for three years (2001-2003). They were arranged in an incomplete two-way classification genotypes by years. Estimates of genotypic values were obtained using the best linear unbiased predictor (BLUP) in a random ANOVA model. Two multivariate methods were used: cluster analysis by the Ward’s procedure (identifying homogenous groups of genotypes) and canonical variate analysis (both detecting characters having the largest discriminant power (relative contribution) in distinguishing the groups and visualisation of multivariate similarities of the identified groups). The first two canonical variables accounted for 87% of the total variation between groups. The first canonical variable was strongly correlated with fruit firmness and fruit size. Therefore these characters have the strongest discriminant power in distinguishing the identified groups.
Strawberry is propagated vegetatively. Nowadays, traditional bare-root transplants are often replaced with plantlets (plug plants) produced from runner tips. The nutrient level and type of growing medium may influence the quality of planting material. Phosphorus and potassium are important elements for plant metabolism. Insufficient supply of plants with these elements negatively influences the rooting and plant vegetative growth. The objective of the study was to evaluate the influence of substrate and monopotassium phosphate (MKP) on rooting and quality of strawberry plantlets grown in the greenhouse soilless system. ‘Elsanta’ mother plants were planted in containers and set on a special rack in the greenhouse. Emerging plantlets were set (clipped with metal clips) in micro-pots filled with peat or coconut substrate. Four concentrations of MKP in the growing medium were applied: 0, 0.22, 0.44, 0.66 g dm-3. Rooting of plantlets was carried out before cutting them off from the mother plants for a period 7, 10 or 14 days. The investigations have showed a very similar dynamics of root formation both in coconut substrate and in peat. A significant effect of the application of monopotassium phosphate on rooting of strawberry plantlets was observed. The optimal dose of MKP depended on the length of rooting period of plantlets. After 7 days of rooting the highest root weight was recorded in case of plantlets rooted in the growing media supplemented with 0.44 or 0.66 g dm-3 MKP. After 10 and 14 days of rooting even the lowest dose of MKP (0.22 g dm-3) resulted in significantly stronger root growth in comparison with not fertilized control plantlets. MKP also significantly influenced the growth of plantlets after transplanting them into bigger containers. The highest quality plantlets were obtained at the dose 0.44 or 0.66 g MKP dm-3 of substrate.
Poland belongs to leading producers of strawberries in Europe and strawberry production has played a significant role in Polish agribusiness for many years. The present study, conducted in a commercial plantation of a specialized horticultural farm during the period 2009–2011, evaluated the amount and quality of yield of two strawberry cultivars (‘Elsanta’ and ‘Albion’) grown under different cropping systems. The study determined the amount and structure of marketable yield. The different production systems included the cultivation of strawberries from fresh waiting-bed plants and frigo plants (class A and A+). Plants were grown in the ground and on black plastic under the row cropping system and the double-row strip cropping system. The average fresh marketable yield for cv. ‘Elsanta’ was 13.0 t·ha-1 and it was higher by 53.0% than the average strawberry yield for cv. ‘Albion’ (7.1 t·ha-1). Treatment D, in which cv. ‘Elsanta’ class A+ frigo plants were grown on black plastic under the double-row strip cropping system, proved to be the best yielding system – the average fresh marketable yield was 14.4 t·ha-1, while the total marketable yield 17.4 t·ha-1. The lowest average fresh marketable yield was obtained from the cv. ‘Albion’ plantation established from class A frigo plants grown in the ground under the double-row strip cropping system – only 4.9 t·ha-1. The percentage of fresh marketable fruits in the total marketable yield ranged from 66.6% for cv. ‘Elsanta’ to 89.6% for cv. ‘Albion’ plants.
This is the first report of somatic embryogenesis from leaves of two strawberry cultivars (Selva and Comarosa) cultured on MS medium containing picloram. Maximum embryogenesis was induced using 2 mg/l picloram. Globular stage embryos developed into cotyledonary ones after transfer to hormone-free media containing 2%, 4%, 6%, 8% and 10% (w/v) sucrose. Increasing sucrose concentrations in culture media enhanced somatic embryo development. Cotyledonary somatic embryos were converted to plantlets after transfer on MS medium containing GA3, and maximum conversion was achieved with 1 mg/l and 2 mg/l GA3. Plantlets were capable of continuous growth under greenhouse conditions.
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