Wyniki wyszukiwania

1

Nowe inhibitory cyklu rozwojowego wirusow rodziny Flaviviridae.

100%

Bretner M., Felczak K., Siwecka M. A., Kulikowski T.

Działalność Naukowa PAN. Wybrane Zagadnienia

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2002

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tom 14

50-52

2

Effect of N-glycosylation inhibition on the synthesis and processing of classical swine fever virus glycoproteins

63%

Tyborowska J., Zdunek E., Szewczyk B.

Acta Biochimica Polonica

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2007

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tom 54

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nr 4

813-819

Classical swine fever virus (CSFV) is often used as a surrogate model in molecular studies of the closely related hepatitis C virus. In this report we have examined the effect of the inhibition of glycosylation on the survival and maturation of CSFV. Viral glycoproteins (Erns, E1, E2) form biologically active complexes — homo- and heterodimers, which are indispensable for viral life cycle. Those complexes are highly N-glycosylated. We studied the influence of N-glycosylation on dimer formation using Erns and E2 glycoproteins produced in insect cells after infection with recombinant baculoviruses. The glycoproteins were efficiently synthesized in insect cells, had similar molecular masses and formed dimers like their natural counterparts. Surprisingly, the addition of tunicamycin (an antibiotic which blocks early steps of glycosylation) to insect cell culture blocked not only dimer formation but it also led to an almost complete disappearance of E2 even in monomeric form. Tunicamycin did not exert a similar effect on the synthesis and formation of Erns dimers; the dimers were still formed, which suggests that Erns glycan chains are not necessary for dimer formation. We have also found that very low doses of tunicamycin (much lower than those used for blocking N-glycosylation) drastically reduced CSFV spread in SK6 (swine kidney) cell culture and the virus yield. These facts indicate that N-glycosylation inhibitors structurally similar to tunicamycin may be potential therapeutics for the inhibition of the spread of CSFV and related viruses

3

Study of the NRT-PCR method for the detection of the West Nile virus

63%

Niczyporuk J. S., Samorek-Salamonowicz E.

Bulletin of the Veterinary Institute in Puławy

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2009

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tom 53

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nr 2

187-192

The aim of the study was to develop RT-PCR and Nested-PCR methods for the detection and identification of the West Nile virus in wild birds. The method was based on West Nile Virus genome non-coding region 3'NCR. All the samples were examined with NRT-PCR and also with a commercial West Nile Virus Kit Prodesse™. There was no positive case of West Nile virus infection in the examined samples. All control samples were positive in both methods. The NRT-PCR method proved to be quick and reliable for the testing of wild birds' tissue samples for WNV.

4

The first detection of the tick-borne encephalitis virus (TBEV) RNA in Dermacentor reticulatus ticks collected from the lowland European bison (Bison bonasus bonasus L.)

63%

Biernat B., Karbowiak G., Stanczak J., Masny A., Werszko J.

Acta Parasitologica

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2016

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tom 61

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nr 1

5

Use of flow cytometry for diagnosis of bovine viral diarrhea virus infection

63%

Rypula K., Matusiewicz K., Chmielewski R., Klimentowski S.

Polish Journal of Veterinary Sciences

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2001

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tom 04

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nr 2

6

Wirus Zachodniego Nilu oraz inne nowo pojawiajace sie zagrozenia zdrowia publicznego

51%

Samorek-Salamonowicz E., Niczyporuk J. S.

Postępy Mikrobiologii

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2010

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tom 49

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nr 3

187-190

7

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Study on the occurrence of tick-borne encephalitis virus RNA in European bison (Bison bonasus) eliminated at Bialowieza Primeval Forest (north-eastern Poland) in 2005-2009

51%

Biernat B., Karbowiak G.

Annals of Parasitology

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2014

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tom 60

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nr 2

Tick-borne encephalitis virus (TBEV) (Flaviviridae, Flavivirus) is an arthropod-borne virus, an etiologic agent of tick-borne encephalitis (TBE), an infection involving the central nervous system. The disease is endemic in a large region in Eurasia where it is transmitted mainly by Ixodes ricinus in Europe and I. persulcatus ticks in Asia. This is the most important tick-transmitted arbovirus of human pathogenicity in Europe. The Białowieża Primeval Forest is a well-known endemic focus of tick-borne encephalitis. The aim of this study was to identify the prevalence of tickborne encephalitis virus (TBEV) in European bison, the important hosts of ticks in the Białowieża Primeval Forest. In the years 2005–2009, 95 blood samples were collected from European bison and examined for the presence of TBEV using nRT-PCR method. No positive results were obtained. For better understanding of TBEV vertebrate reservoir hosts in Poland, further investigations are needed.

8

NTPase-helicase of Flaviviridae: inhibitors and inhibition of the enzyme

51%

Borowski P., Niebuhr A., Schmitz H., Hosmane R. S., Bretner M., Siwecka M. A., Kulikowski T.

Acta Biochimica Polonica

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2002

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tom 49

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nr 3

RNA nucleoside triphosphatases (NTPase)/helicases represent a large family of proteins that are ubiquitously distributed over a wide range of organisms. The enzymes play essential role in cell development and differentiation, and some of them are involved in transcription and replication of viral single-stranded RNA genomes. The enzymatic activities of a NTPase/helicase were also detected in the carboxyl-terminal non-structural protein 3 (NS3) of members of the Flaviviridae family. The crucial role of the enzyme for the virus life cycle was demonstrated in knock out experiments and by using NTPase/helicase specific inhibitors. This makes the enzyme an attractive target for development of Flaviviridae-specific antiviral therapies. This review will sum-marize our knowledge about the function and structure of the enzyme, update the spectrum of inhibitors of the enzymatic activities of the NTPase/helicase and describe the different mechanisms by which the compounds act. Some of the compounds reviewed herein could show potential utility as antiviral agents against Flaviviridae viruses.

Ograniczanie wyników

Nowe inhibitory cyklu rozwojowego wirusow rodziny Flaviviridae.

Effect of N-glycosylation inhibition on the synthesis and processing of classical swine fever virus glycoproteins

Study of the NRT-PCR method for the detection of the West Nile virus

The first detection of the tick-borne encephalitis virus (TBEV) RNA in Dermacentor reticulatus ticks collected from the lowland European bison (Bison bonasus bonasus L.)

Use of flow cytometry for diagnosis of bovine viral diarrhea virus infection

Wirus Zachodniego Nilu oraz inne nowo pojawiajace sie zagrozenia zdrowia publicznego

Study on the occurrence of tick-borne encephalitis virus RNA in European bison (Bison bonasus) eliminated at Bialowieza Primeval Forest (north-eastern Poland) in 2005-2009

NTPase-helicase of Flaviviridae: inhibitors and inhibition of the enzyme