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Japanese plum trees of the cultivars 'Kometa', 'Najdiena', 'Skoroplodnaja' and 'Shiro' grafted on Wangenheim Prune seedlings, were planted in the spring of 2006 at the Fruit Experimental Station in Samotwor near Wroc law, Poland. As the control, trees of the 'Amers' cultivar, which belong to European plums, were used. All trees were planted at a spacing of 4.0 x 2.0 m (1250 trees per hectare). The experiment was established in a randomized block design, in four replications, with 5 trees per plot. In 2006-2009, records of vegetative growth, abundance of blooming, yield, and fruit weight were taken. The first significant yield was achieved in the third year after planting. In 2008­2009 trees of 'Shiro' had an abundant yield (48.5 kg •tree-1). 'Shiro' produced a sig­nificantly higher yield compared to other cultivars. There were no significant differ­ences between 'Amers' (27.3 kg •tree-1) and the Japanese plum cultivars 'Kometa' (29.2 kg-tree-1) and 'Skoroplodnaja' (21.5 kg •tree-1). Japanese plum fruits were sig­nificantly smaller compared to the European ones. The cultivar 'Amers' had the heaviest fruit (53 g). The Japanese plum trees had smaller fruits, and among the them, the cultivar 'Shiro' had the largest fruit (44 g). The observations showed that 'Shiro' produced higher yields, gave large fruits, and had intensive blooming, but the growth of the trees was very strong.
Despite senescence-induced chlorophyll depletion in plants has been widely studied, the enzymatic background of this physiologically regulated process still remains highly unclear. The purpose of this study was to determine selected biochemical properties of partially purified fractions of chlorophyllase (Chlase, chlorophyll chlorophyllido-hydrolase, EC 3.1.1.14) from leaves of three Prunus species: bird cherry (Prunus padus L.), European plum (Prunus domestica L.), and sour cherry (Prunus cerasus L.). Secondarily, this report was aimed at comparing seasonal dynamics of Chlase activity and chlorophyll a (Chl a) content within investigated plant systems. Molecular weight of native Chlase F1 has been estimated at 90 kDa (bird cherry) and approximately 100 kDa (European plum and sour cherry), whereas molecular mass of Chlase F2 varied from 35 kDa (European plum) to 60 kDa (sour cherry). Furthermore, enzyme fractions possessed similar optimal pH values ranging from 7.6 to 8.0. It was found that among a broad panel of tested metal ions, Hg+2, Fe+2, and Cu+2 cations showed the most pronounced inhibitory effect on the activity of Chlase. In contrast, the presence of Mg+2 ions influenced a subtle stimulation of the enzymatic activity. Importantly, although Chlase activity was negatively correlated with the amount of Chl a in leaves of examined Prunus species, detailed comparative analyses revealed an incidental decrement of enzymatic activity in early or moderately senescing leaves. It provides evidence that foliar Chlase is not the only enzyme involved in autumnal chlorophyll breakdown and further in-depth studies elucidating this catabolic process are required.
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